Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2897 and Pf6N2E2_2898 overlap by 4 nucleotides Pf6N2E2_2898 and Pf6N2E2_2899 are separated by 218 nucleotides
Pf6N2E2_2897 - Glucokinase (EC 2.7.1.2), at 3,385,930 to 3,386,889
_2897
Pf6N2E2_2898 - Phosphogluconate dehydratase (EC 4.2.1.12), at 3,386,886 to 3,388,712
_2898
Pf6N2E2_2899 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 3,388,931 to 3,389,932
_2899
Position (kb)
3386
3387
3388
3389 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3385.913 kb on - strand at 3385.913 kb on - strand at 3385.913 kb on - strand at 3385.922 kb on - strand at 3386.022 kb on - strand at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.115 kb on - strand, within Pf6N2E2_2897 at 3386.115 kb on - strand, within Pf6N2E2_2897 at 3386.115 kb on - strand, within Pf6N2E2_2897 at 3386.115 kb on - strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.355 kb on - strand, within Pf6N2E2_2897 at 3386.355 kb on - strand, within Pf6N2E2_2897 at 3386.463 kb on + strand, within Pf6N2E2_2897 at 3386.463 kb on + strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.533 kb on + strand, within Pf6N2E2_2897 at 3386.533 kb on + strand, within Pf6N2E2_2897 at 3386.667 kb on + strand, within Pf6N2E2_2897 at 3386.756 kb on + strand, within Pf6N2E2_2897 at 3386.757 kb on - strand, within Pf6N2E2_2897 at 3386.786 kb on + strand, within Pf6N2E2_2897 at 3386.787 kb on - strand, within Pf6N2E2_2897 at 3386.892 kb on - strand at 3386.892 kb on - strand at 3386.892 kb on - strand at 3386.892 kb on - strand at 3388.755 kb on - strand at 3388.755 kb on - strand at 3388.755 kb on - strand at 3388.889 kb on + strand at 3388.994 kb on + strand at 3388.994 kb on + strand at 3388.995 kb on - strand at 3388.995 kb on - strand at 3388.995 kb on - strand at 3388.995 kb on - strand at 3388.995 kb on - strand at 3389.266 kb on + strand, within Pf6N2E2_2899 at 3389.267 kb on - strand, within Pf6N2E2_2899 at 3389.267 kb on - strand, within Pf6N2E2_2899 at 3389.333 kb on + strand, within Pf6N2E2_2899 at 3389.477 kb on + strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.507 kb on + strand, within Pf6N2E2_2899 at 3389.508 kb on - strand, within Pf6N2E2_2899
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 3,385,913 - -0.1 3,385,913 - -0.5 3,385,913 - -0.2 3,385,922 - +0.7 3,386,022 - -0.7 3,386,114 + Pf6N2E2_2897 0.19 -0.7 3,386,114 + Pf6N2E2_2897 0.19 -0.0 3,386,114 + Pf6N2E2_2897 0.19 -0.3 3,386,114 + Pf6N2E2_2897 0.19 -0.4 3,386,114 + Pf6N2E2_2897 0.19 -0.6 3,386,114 + Pf6N2E2_2897 0.19 -0.4 3,386,115 - Pf6N2E2_2897 0.19 -1.2 3,386,115 - Pf6N2E2_2897 0.19 +0.3 3,386,115 - Pf6N2E2_2897 0.19 +0.2 3,386,115 - Pf6N2E2_2897 0.19 -0.2 3,386,354 + Pf6N2E2_2897 0.44 +0.0 3,386,354 + Pf6N2E2_2897 0.44 -0.2 3,386,354 + Pf6N2E2_2897 0.44 -0.2 3,386,354 + Pf6N2E2_2897 0.44 +0.6 3,386,354 + Pf6N2E2_2897 0.44 +0.7 3,386,354 + Pf6N2E2_2897 0.44 +3.0 3,386,355 - Pf6N2E2_2897 0.44 +0.5 3,386,355 - Pf6N2E2_2897 0.44 +0.5 3,386,463 + Pf6N2E2_2897 0.56 +1.4 3,386,463 + Pf6N2E2_2897 0.56 +0.7 3,386,464 - Pf6N2E2_2897 0.56 -1.2 3,386,464 - Pf6N2E2_2897 0.56 -1.5 3,386,464 - Pf6N2E2_2897 0.56 +1.4 3,386,464 - Pf6N2E2_2897 0.56 +0.2 3,386,464 - Pf6N2E2_2897 0.56 -0.8 3,386,533 + Pf6N2E2_2897 0.63 -0.9 3,386,533 + Pf6N2E2_2897 0.63 -1.3 3,386,667 + Pf6N2E2_2897 0.77 -0.2 3,386,756 + Pf6N2E2_2897 0.86 +0.0 3,386,757 - Pf6N2E2_2897 0.86 +0.1 3,386,786 + Pf6N2E2_2897 0.89 +1.4 3,386,787 - Pf6N2E2_2897 0.89 -0.4 3,386,892 - -0.9 3,386,892 - +1.0 3,386,892 - +0.3 3,386,892 - -0.5 3,388,755 - +0.4 3,388,755 - -0.1 3,388,755 - +0.3 3,388,889 + +0.1 3,388,994 + +3.0 3,388,994 + +1.2 3,388,995 - +0.7 3,388,995 - +0.6 3,388,995 - +0.6 3,388,995 - +1.4 3,388,995 - +1.4 3,389,266 + Pf6N2E2_2899 0.33 -0.5 3,389,267 - Pf6N2E2_2899 0.34 +1.4 3,389,267 - Pf6N2E2_2899 0.34 +0.9 3,389,333 + Pf6N2E2_2899 0.40 +0.7 3,389,477 + Pf6N2E2_2899 0.54 +0.2 3,389,478 - Pf6N2E2_2899 0.55 +0.9 3,389,478 - Pf6N2E2_2899 0.55 -0.2 3,389,478 - Pf6N2E2_2899 0.55 +0.4 3,389,478 - Pf6N2E2_2899 0.55 -0.6 3,389,507 + Pf6N2E2_2899 0.57 +0.0 3,389,508 - Pf6N2E2_2899 0.58 -0.9
Or see this region's nucleotide sequence