Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2801 and Pf6N2E2_2802 are separated by 140 nucleotides Pf6N2E2_2802 and Pf6N2E2_2803 are separated by 140 nucleotides
Pf6N2E2_2801 - Bicyclomycin resistance protein, at 3,287,048 to 3,288,211
_2801
Pf6N2E2_2802 - Alcohol dehydrogenase (EC 1.1.1.1), at 3,288,352 to 3,289,500
_2802
Pf6N2E2_2803 - FIG00953317: hypothetical protein, at 3,289,641 to 3,290,660
_2803
Position (kb)
3288
3289
3290 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3287.486 kb on + strand, within Pf6N2E2_2801 at 3287.486 kb on + strand, within Pf6N2E2_2801 at 3287.487 kb on - strand, within Pf6N2E2_2801 at 3287.636 kb on + strand, within Pf6N2E2_2801 at 3287.637 kb on - strand, within Pf6N2E2_2801 at 3287.637 kb on - strand, within Pf6N2E2_2801 at 3287.771 kb on + strand, within Pf6N2E2_2801 at 3287.771 kb on + strand, within Pf6N2E2_2801 at 3287.772 kb on - strand, within Pf6N2E2_2801 at 3287.804 kb on + strand, within Pf6N2E2_2801 at 3287.804 kb on + strand, within Pf6N2E2_2801 at 3287.804 kb on + strand, within Pf6N2E2_2801 at 3287.804 kb on + strand, within Pf6N2E2_2801 at 3287.805 kb on - strand, within Pf6N2E2_2801 at 3287.838 kb on + strand, within Pf6N2E2_2801 at 3287.839 kb on - strand, within Pf6N2E2_2801 at 3287.993 kb on + strand, within Pf6N2E2_2801 at 3287.993 kb on + strand, within Pf6N2E2_2801 at 3287.993 kb on + strand, within Pf6N2E2_2801 at 3287.994 kb on - strand, within Pf6N2E2_2801 at 3288.045 kb on - strand, within Pf6N2E2_2801 at 3288.219 kb on - strand at 3288.344 kb on + strand at 3288.344 kb on + strand at 3288.344 kb on + strand at 3288.345 kb on - strand at 3288.345 kb on - strand at 3288.353 kb on + strand at 3288.353 kb on + strand at 3288.353 kb on + strand at 3288.353 kb on + strand at 3288.354 kb on - strand at 3288.455 kb on - strand at 3288.469 kb on + strand, within Pf6N2E2_2802 at 3288.469 kb on + strand, within Pf6N2E2_2802 at 3288.470 kb on - strand, within Pf6N2E2_2802 at 3288.574 kb on - strand, within Pf6N2E2_2802 at 3288.686 kb on + strand, within Pf6N2E2_2802 at 3288.686 kb on + strand, within Pf6N2E2_2802 at 3288.686 kb on + strand, within Pf6N2E2_2802 at 3288.686 kb on + strand, within Pf6N2E2_2802 at 3288.686 kb on + strand, within Pf6N2E2_2802 at 3288.687 kb on - strand, within Pf6N2E2_2802 at 3288.687 kb on - strand, within Pf6N2E2_2802 at 3288.687 kb on - strand, within Pf6N2E2_2802 at 3288.771 kb on - strand, within Pf6N2E2_2802 at 3288.771 kb on - strand, within Pf6N2E2_2802 at 3288.896 kb on + strand, within Pf6N2E2_2802 at 3288.896 kb on + strand, within Pf6N2E2_2802 at 3288.896 kb on + strand, within Pf6N2E2_2802 at 3288.896 kb on + strand, within Pf6N2E2_2802 at 3288.896 kb on + strand, within Pf6N2E2_2802 at 3288.896 kb on + strand, within Pf6N2E2_2802 at 3288.896 kb on + strand, within Pf6N2E2_2802 at 3288.896 kb on + strand, within Pf6N2E2_2802 at 3288.897 kb on - strand, within Pf6N2E2_2802 at 3288.897 kb on - strand, within Pf6N2E2_2802 at 3289.145 kb on + strand, within Pf6N2E2_2802 at 3289.146 kb on - strand, within Pf6N2E2_2802 at 3289.146 kb on - strand, within Pf6N2E2_2802 at 3289.146 kb on - strand, within Pf6N2E2_2802 at 3289.554 kb on + strand at 3289.659 kb on + strand at 3289.659 kb on + strand at 3289.944 kb on + strand, within Pf6N2E2_2803 at 3289.945 kb on - strand, within Pf6N2E2_2803 at 3290.074 kb on - strand, within Pf6N2E2_2803 at 3290.074 kb on - strand, within Pf6N2E2_2803 at 3290.074 kb on - strand, within Pf6N2E2_2803 at 3290.193 kb on + strand, within Pf6N2E2_2803 at 3290.193 kb on + strand, within Pf6N2E2_2803 at 3290.379 kb on + strand, within Pf6N2E2_2803 at 3290.380 kb on - strand, within Pf6N2E2_2803 at 3290.380 kb on - strand, within Pf6N2E2_2803 at 3290.380 kb on - strand, within Pf6N2E2_2803 at 3290.380 kb on - strand, within Pf6N2E2_2803 at 3290.394 kb on + strand, within Pf6N2E2_2803
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 3,287,486 + Pf6N2E2_2801 0.38 -0.6 3,287,486 + Pf6N2E2_2801 0.38 +0.5 3,287,487 - Pf6N2E2_2801 0.38 -0.1 3,287,636 + Pf6N2E2_2801 0.51 -2.4 3,287,637 - Pf6N2E2_2801 0.51 +0.4 3,287,637 - Pf6N2E2_2801 0.51 -1.1 3,287,771 + Pf6N2E2_2801 0.62 +0.5 3,287,771 + Pf6N2E2_2801 0.62 +0.6 3,287,772 - Pf6N2E2_2801 0.62 -0.4 3,287,804 + Pf6N2E2_2801 0.65 +0.7 3,287,804 + Pf6N2E2_2801 0.65 -0.2 3,287,804 + Pf6N2E2_2801 0.65 -0.2 3,287,804 + Pf6N2E2_2801 0.65 +0.1 3,287,805 - Pf6N2E2_2801 0.65 +0.3 3,287,838 + Pf6N2E2_2801 0.68 +1.4 3,287,839 - Pf6N2E2_2801 0.68 -1.3 3,287,993 + Pf6N2E2_2801 0.81 -0.3 3,287,993 + Pf6N2E2_2801 0.81 +0.6 3,287,993 + Pf6N2E2_2801 0.81 +0.9 3,287,994 - Pf6N2E2_2801 0.81 -0.6 3,288,045 - Pf6N2E2_2801 0.86 -2.7 3,288,219 - -0.3 3,288,344 + -0.5 3,288,344 + +0.2 3,288,344 + +0.6 3,288,345 - +0.5 3,288,345 - +0.9 3,288,353 + +0.3 3,288,353 + +0.9 3,288,353 + +0.3 3,288,353 + +0.4 3,288,354 - +0.3 3,288,455 - +1.0 3,288,469 + Pf6N2E2_2802 0.10 +0.9 3,288,469 + Pf6N2E2_2802 0.10 +1.4 3,288,470 - Pf6N2E2_2802 0.10 +0.3 3,288,574 - Pf6N2E2_2802 0.19 +1.2 3,288,686 + Pf6N2E2_2802 0.29 +0.7 3,288,686 + Pf6N2E2_2802 0.29 +1.2 3,288,686 + Pf6N2E2_2802 0.29 +1.5 3,288,686 + Pf6N2E2_2802 0.29 +0.5 3,288,686 + Pf6N2E2_2802 0.29 +0.9 3,288,687 - Pf6N2E2_2802 0.29 +2.0 3,288,687 - Pf6N2E2_2802 0.29 +1.0 3,288,687 - Pf6N2E2_2802 0.29 +1.0 3,288,771 - Pf6N2E2_2802 0.36 +0.2 3,288,771 - Pf6N2E2_2802 0.36 +0.6 3,288,896 + Pf6N2E2_2802 0.47 +1.0 3,288,896 + Pf6N2E2_2802 0.47 +1.3 3,288,896 + Pf6N2E2_2802 0.47 +1.0 3,288,896 + Pf6N2E2_2802 0.47 +0.8 3,288,896 + Pf6N2E2_2802 0.47 -1.2 3,288,896 + Pf6N2E2_2802 0.47 +1.1 3,288,896 + Pf6N2E2_2802 0.47 +0.9 3,288,896 + Pf6N2E2_2802 0.47 +1.0 3,288,897 - Pf6N2E2_2802 0.47 +1.7 3,288,897 - Pf6N2E2_2802 0.47 +2.3 3,289,145 + Pf6N2E2_2802 0.69 +1.5 3,289,146 - Pf6N2E2_2802 0.69 +0.8 3,289,146 - Pf6N2E2_2802 0.69 +1.7 3,289,146 - Pf6N2E2_2802 0.69 +1.4 3,289,554 + +1.5 3,289,659 + +0.1 3,289,659 + +0.1 3,289,944 + Pf6N2E2_2803 0.30 +0.2 3,289,945 - Pf6N2E2_2803 0.30 -0.1 3,290,074 - Pf6N2E2_2803 0.42 +1.4 3,290,074 - Pf6N2E2_2803 0.42 +0.5 3,290,074 - Pf6N2E2_2803 0.42 -0.6 3,290,193 + Pf6N2E2_2803 0.54 -0.0 3,290,193 + Pf6N2E2_2803 0.54 +0.6 3,290,379 + Pf6N2E2_2803 0.72 -0.2 3,290,380 - Pf6N2E2_2803 0.72 +0.1 3,290,380 - Pf6N2E2_2803 0.72 -1.1 3,290,380 - Pf6N2E2_2803 0.72 -0.1 3,290,380 - Pf6N2E2_2803 0.72 -0.5 3,290,394 + Pf6N2E2_2803 0.74 +0.5
Or see this region's nucleotide sequence