Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2623 and Pf6N2E2_2624 are separated by 10 nucleotides Pf6N2E2_2624 and Pf6N2E2_2625 are separated by 11 nucleotides
Pf6N2E2_2623 - Flagellar hook-associated protein FlgK, at 3,083,568 to 3,085,634
_2623
Pf6N2E2_2624 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 3,085,645 to 3,086,949
_2624
Pf6N2E2_2625 - Flagellar P-ring protein FlgI, at 3,086,961 to 3,088,067
_2625
Position (kb)
3085
3086
3087 Strain fitness (log2 ratio)
-1
0
1
2 at 3084.964 kb on + strand, within Pf6N2E2_2623 at 3084.964 kb on + strand, within Pf6N2E2_2623 at 3084.965 kb on - strand, within Pf6N2E2_2623 at 3084.965 kb on - strand, within Pf6N2E2_2623 at 3084.965 kb on - strand, within Pf6N2E2_2623 at 3085.429 kb on + strand at 3085.436 kb on + strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.438 kb on + strand at 3085.438 kb on + strand at 3085.438 kb on + strand at 3085.438 kb on + strand at 3085.438 kb on + strand at 3085.439 kb on - strand at 3085.537 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.620 kb on - strand at 3085.646 kb on + strand at 3085.646 kb on + strand at 3085.647 kb on - strand at 3085.647 kb on - strand at 3085.647 kb on - strand at 3085.647 kb on - strand at 3085.648 kb on + strand at 3085.648 kb on + strand at 3085.648 kb on + strand at 3085.648 kb on + strand at 3085.649 kb on - strand at 3085.649 kb on - strand at 3085.649 kb on - strand at 3085.705 kb on + strand at 3085.705 kb on + strand at 3085.706 kb on - strand at 3085.727 kb on + strand at 3085.728 kb on - strand at 3085.728 kb on - strand at 3085.745 kb on + strand at 3085.863 kb on - strand, within Pf6N2E2_2624 at 3085.872 kb on - strand, within Pf6N2E2_2624 at 3085.872 kb on - strand, within Pf6N2E2_2624 at 3086.061 kb on - strand, within Pf6N2E2_2624 at 3086.189 kb on + strand, within Pf6N2E2_2624 at 3086.448 kb on + strand, within Pf6N2E2_2624 at 3086.520 kb on + strand, within Pf6N2E2_2624 at 3086.520 kb on + strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.708 kb on + strand, within Pf6N2E2_2624 at 3086.709 kb on - strand, within Pf6N2E2_2624 at 3086.709 kb on - strand, within Pf6N2E2_2624 at 3086.720 kb on + strand, within Pf6N2E2_2624 at 3086.901 kb on - strand at 3086.901 kb on - strand at 3086.940 kb on + strand at 3086.941 kb on - strand at 3086.962 kb on + strand at 3086.962 kb on + strand at 3086.962 kb on + strand at 3086.963 kb on - strand at 3086.963 kb on - strand at 3087.352 kb on + strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.658 kb on + strand, within Pf6N2E2_2625 at 3087.658 kb on + strand, within Pf6N2E2_2625
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 3,084,964 + Pf6N2E2_2623 0.68 +0.9 3,084,964 + Pf6N2E2_2623 0.68 +0.4 3,084,965 - Pf6N2E2_2623 0.68 +0.8 3,084,965 - Pf6N2E2_2623 0.68 -0.1 3,084,965 - Pf6N2E2_2623 0.68 +2.4 3,085,429 + +2.2 3,085,436 + +0.8 3,085,437 - +1.4 3,085,437 - +0.5 3,085,437 - +0.3 3,085,437 - +0.4 3,085,437 - +1.1 3,085,437 - +0.5 3,085,438 + +1.3 3,085,438 + +1.4 3,085,438 + +0.3 3,085,438 + +0.0 3,085,438 + +0.9 3,085,439 - +1.0 3,085,537 + +0.7 3,085,617 + +0.4 3,085,617 + +1.0 3,085,617 + +0.9 3,085,617 + +2.0 3,085,617 + +0.6 3,085,617 + +0.4 3,085,617 + +0.9 3,085,618 - +0.0 3,085,618 - +1.4 3,085,618 - +1.1 3,085,618 - -1.3 3,085,618 - +0.4 3,085,618 - +1.3 3,085,620 - +0.7 3,085,646 + +1.4 3,085,646 + +1.0 3,085,647 - +0.5 3,085,647 - +0.1 3,085,647 - +1.6 3,085,647 - +0.2 3,085,648 + +1.0 3,085,648 + +0.5 3,085,648 + +0.6 3,085,648 + +0.3 3,085,649 - +0.0 3,085,649 - +0.4 3,085,649 - +0.2 3,085,705 + -0.1 3,085,705 + +1.3 3,085,706 - -1.2 3,085,727 + +0.9 3,085,728 - +0.9 3,085,728 - +0.9 3,085,745 + +1.7 3,085,863 - Pf6N2E2_2624 0.17 +1.2 3,085,872 - Pf6N2E2_2624 0.17 +1.5 3,085,872 - Pf6N2E2_2624 0.17 +1.7 3,086,061 - Pf6N2E2_2624 0.32 +1.1 3,086,189 + Pf6N2E2_2624 0.42 +1.3 3,086,448 + Pf6N2E2_2624 0.62 +1.5 3,086,520 + Pf6N2E2_2624 0.67 -0.2 3,086,520 + Pf6N2E2_2624 0.67 +1.5 3,086,521 - Pf6N2E2_2624 0.67 +1.5 3,086,521 - Pf6N2E2_2624 0.67 +0.8 3,086,521 - Pf6N2E2_2624 0.67 +1.6 3,086,521 - Pf6N2E2_2624 0.67 +1.9 3,086,708 + Pf6N2E2_2624 0.81 +0.5 3,086,709 - Pf6N2E2_2624 0.82 +2.4 3,086,709 - Pf6N2E2_2624 0.82 +1.0 3,086,720 + Pf6N2E2_2624 0.82 +0.9 3,086,901 - +0.4 3,086,901 - +1.2 3,086,940 + +0.7 3,086,941 - +1.8 3,086,962 + +0.8 3,086,962 + +0.7 3,086,962 + +0.9 3,086,963 - +0.2 3,086,963 - +0.8 3,087,352 + Pf6N2E2_2625 0.35 +1.4 3,087,353 - Pf6N2E2_2625 0.35 +2.0 3,087,353 - Pf6N2E2_2625 0.35 +0.9 3,087,353 - Pf6N2E2_2625 0.35 +1.7 3,087,353 - Pf6N2E2_2625 0.35 +1.6 3,087,658 + Pf6N2E2_2625 0.63 +2.0 3,087,658 + Pf6N2E2_2625 0.63 +1.6
Or see this region's nucleotide sequence