Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2571 and Pf6N2E2_2572 are separated by 36 nucleotides Pf6N2E2_2572 and Pf6N2E2_2573 are separated by 155 nucleotides Pf6N2E2_2573 and Pf6N2E2_2574 overlap by 4 nucleotides Pf6N2E2_2574 and Pf6N2E2_2575 are separated by 116 nucleotides
Pf6N2E2_2571 - Chemotaxis response - phosphatase CheZ, at 3,029,860 to 3,030,627
_2571
Pf6N2E2_2572 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 3,030,664 to 3,031,047
_2572
Pf6N2E2_2573 - RNA polymerase sigma factor for flagellar operon, at 3,031,203 to 3,031,946
_2573
Pf6N2E2_2574 - Flagellar synthesis regulator FleN, at 3,031,943 to 3,032,773
_2574
Pf6N2E2_2575 - Flagellar biosynthesis protein FlhF, at 3,032,890 to 3,034,239
_2575
Position (kb)
3031
3032 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3030.377 kb on + strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.645 kb on + strand at 3030.645 kb on + strand at 3030.645 kb on + strand at 3030.645 kb on + strand at 3030.646 kb on - strand at 3030.646 kb on - strand at 3030.734 kb on + strand, within Pf6N2E2_2572 at 3030.735 kb on - strand, within Pf6N2E2_2572 at 3030.743 kb on - strand, within Pf6N2E2_2572 at 3030.988 kb on + strand, within Pf6N2E2_2572 at 3031.067 kb on + strand at 3031.067 kb on + strand at 3031.068 kb on - strand at 3031.154 kb on + strand at 3031.155 kb on - strand at 3031.155 kb on - strand at 3031.261 kb on - strand at 3031.261 kb on - strand at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.520 kb on + strand, within Pf6N2E2_2573 at 3031.520 kb on + strand, within Pf6N2E2_2573 at 3031.521 kb on - strand, within Pf6N2E2_2573 at 3031.540 kb on + strand, within Pf6N2E2_2573 at 3031.586 kb on + strand, within Pf6N2E2_2573 at 3031.604 kb on + strand, within Pf6N2E2_2573 at 3031.644 kb on - strand, within Pf6N2E2_2573 at 3031.723 kb on + strand, within Pf6N2E2_2573 at 3031.723 kb on + strand, within Pf6N2E2_2573 at 3031.724 kb on - strand, within Pf6N2E2_2573 at 3031.724 kb on - strand, within Pf6N2E2_2573 at 3031.724 kb on - strand, within Pf6N2E2_2573 at 3031.757 kb on - strand, within Pf6N2E2_2573 at 3031.846 kb on + strand, within Pf6N2E2_2573 at 3031.846 kb on + strand, within Pf6N2E2_2573 at 3031.847 kb on - strand, within Pf6N2E2_2573 at 3031.892 kb on - strand at 3031.919 kb on - strand at 3031.919 kb on - strand at 3031.931 kb on - strand at 3031.931 kb on - strand at 3031.947 kb on - strand at 3032.154 kb on + strand, within Pf6N2E2_2574 at 3032.154 kb on + strand, within Pf6N2E2_2574 at 3032.271 kb on + strand, within Pf6N2E2_2574 at 3032.795 kb on - strand at 3032.795 kb on - strand at 3032.798 kb on + strand at 3032.799 kb on - strand at 3032.833 kb on + strand at 3032.834 kb on - strand at 3032.834 kb on - strand at 3032.834 kb on - strand at 3032.863 kb on + strand at 3032.863 kb on + strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.864 kb on - strand at 3032.868 kb on - strand at 3032.887 kb on - strand at 3032.887 kb on - strand at 3032.887 kb on - strand at 3032.887 kb on - strand at 3032.918 kb on + strand at 3032.919 kb on - strand at 3032.919 kb on - strand at 3032.919 kb on - strand at 3032.919 kb on - strand at 3032.923 kb on - strand at 3032.923 kb on - strand at 3032.923 kb on - strand at 3032.935 kb on - strand at 3032.935 kb on - strand at 3032.935 kb on - strand at 3032.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 3,030,377 + Pf6N2E2_2571 0.67 -1.1 3,030,378 - Pf6N2E2_2571 0.67 -0.0 3,030,378 - Pf6N2E2_2571 0.67 -0.6 3,030,378 - Pf6N2E2_2571 0.67 -0.5 3,030,378 - Pf6N2E2_2571 0.67 +0.1 3,030,378 - Pf6N2E2_2571 0.67 -0.8 3,030,378 - Pf6N2E2_2571 0.67 +0.2 3,030,645 + +0.3 3,030,645 + +0.1 3,030,645 + -0.7 3,030,645 + -0.3 3,030,646 - -1.4 3,030,646 - +1.4 3,030,734 + Pf6N2E2_2572 0.18 +1.4 3,030,735 - Pf6N2E2_2572 0.18 -1.9 3,030,743 - Pf6N2E2_2572 0.21 -1.0 3,030,988 + Pf6N2E2_2572 0.84 -1.6 3,031,067 + -0.3 3,031,067 + -0.9 3,031,068 - -0.1 3,031,154 + -1.3 3,031,155 - -2.1 3,031,155 - -0.9 3,031,261 - +0.5 3,031,261 - +0.4 3,031,334 - Pf6N2E2_2573 0.18 +1.3 3,031,334 - Pf6N2E2_2573 0.18 +0.9 3,031,334 - Pf6N2E2_2573 0.18 +1.7 3,031,520 + Pf6N2E2_2573 0.43 +1.8 3,031,520 + Pf6N2E2_2573 0.43 +0.6 3,031,521 - Pf6N2E2_2573 0.43 +0.8 3,031,540 + Pf6N2E2_2573 0.45 +0.4 3,031,586 + Pf6N2E2_2573 0.51 -0.2 3,031,604 + Pf6N2E2_2573 0.54 +1.7 3,031,644 - Pf6N2E2_2573 0.59 +0.8 3,031,723 + Pf6N2E2_2573 0.70 +2.4 3,031,723 + Pf6N2E2_2573 0.70 +1.4 3,031,724 - Pf6N2E2_2573 0.70 +1.3 3,031,724 - Pf6N2E2_2573 0.70 +0.2 3,031,724 - Pf6N2E2_2573 0.70 +1.3 3,031,757 - Pf6N2E2_2573 0.74 +0.4 3,031,846 + Pf6N2E2_2573 0.86 +1.2 3,031,846 + Pf6N2E2_2573 0.86 +1.3 3,031,847 - Pf6N2E2_2573 0.87 +1.6 3,031,892 - +1.5 3,031,919 - +0.9 3,031,919 - +0.4 3,031,931 - +1.4 3,031,931 - +1.8 3,031,947 - +2.4 3,032,154 + Pf6N2E2_2574 0.25 -0.9 3,032,154 + Pf6N2E2_2574 0.25 -0.2 3,032,271 + Pf6N2E2_2574 0.39 -0.9 3,032,795 - +0.7 3,032,795 - +1.2 3,032,798 + +1.4 3,032,799 - +0.9 3,032,833 + +0.8 3,032,834 - +1.4 3,032,834 - +0.6 3,032,834 - -0.3 3,032,863 + -2.1 3,032,863 + +0.8 3,032,864 - -1.0 3,032,864 - +0.5 3,032,864 - -1.1 3,032,864 - +0.7 3,032,864 - -0.4 3,032,864 - -0.4 3,032,864 - +1.1 3,032,864 - -0.3 3,032,868 - +0.4 3,032,887 - -0.1 3,032,887 - +1.4 3,032,887 - +1.1 3,032,887 - +1.4 3,032,918 + -0.6 3,032,919 - +1.2 3,032,919 - +1.0 3,032,919 - +0.8 3,032,919 - +1.0 3,032,923 - +0.7 3,032,923 - +1.1 3,032,923 - +0.5 3,032,935 - +1.1 3,032,935 - +1.6 3,032,935 - +0.8 3,032,935 - +0.7
Or see this region's nucleotide sequence