Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1162

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
1,359,058	+				-1.8
1,359,058	+				+0.3
1,359,100	+				-0.1
1,359,100	+				+1.0
1,359,101	-				+1.5
1,359,158	+				-0.5
1,359,191	+				+0.4
1,359,191	+				+0.3
1,359,191	+				+1.2
1,359,404	+		Pf6N2E2_1161	0.21	+1.5
1,359,404	+		Pf6N2E2_1161	0.21	-0.5
1,359,414	+		Pf6N2E2_1161	0.22	-0.1
1,359,414	+		Pf6N2E2_1161	0.22	+0.3
1,359,415	-		Pf6N2E2_1161	0.22	-0.2
1,359,415	-		Pf6N2E2_1161	0.22	-0.8
1,359,650	+		Pf6N2E2_1161	0.57	+0.3
1,359,651	-		Pf6N2E2_1161	0.57	-0.5
1,359,651	-		Pf6N2E2_1161	0.57	+0.9
1,359,824	-		Pf6N2E2_1161	0.83	+0.1
1,359,824	-		Pf6N2E2_1161	0.83	+0.6
1,359,974	+				+1.5
1,360,071	-		Pf6N2E2_1162	0.10	-0.2
1,360,071	-		Pf6N2E2_1162	0.10	-1.5
1,360,071	-		Pf6N2E2_1162	0.10	-1.8
1,360,515	+		Pf6N2E2_1162	0.81	+1.5
1,360,613	+				+0.2
1,360,613	+				-0.2
1,360,614	-				-0.6
1,360,891	-				-0.3
1,360,952	+		Pf6N2E2_1163	0.12	+1.3
1,361,077	-		Pf6N2E2_1163	0.21	+0.2
1,361,406	-		Pf6N2E2_1163	0.45	-0.4
1,361,632	+		Pf6N2E2_1163	0.61	+2.5