Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1016 and Pf6N2E2_1017 overlap by 4 nucleotides Pf6N2E2_1017 and Pf6N2E2_1018 are separated by 124 nucleotides
Pf6N2E2_1016 - Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin, at 1,185,906 to 1,187,060
_1016
Pf6N2E2_1017 - small molecule metabolism, at 1,187,057 to 1,187,905
_1017
Pf6N2E2_1018 - putative DNA-binding protein, at 1,188,030 to 1,189,055
_1018
Position (kb)
1188
1189
1190 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1187.430 kb on + strand, within Pf6N2E2_1017 at 1187.865 kb on + strand at 1187.865 kb on + strand at 1187.885 kb on - strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.922 kb on - strand at 1187.968 kb on + strand at 1187.969 kb on - strand at 1188.112 kb on + strand at 1188.113 kb on - strand at 1188.113 kb on - strand at 1188.135 kb on + strand, within Pf6N2E2_1018 at 1188.212 kb on + strand, within Pf6N2E2_1018 at 1188.212 kb on + strand, within Pf6N2E2_1018 at 1188.213 kb on - strand, within Pf6N2E2_1018 at 1188.213 kb on - strand, within Pf6N2E2_1018 at 1188.213 kb on - strand, within Pf6N2E2_1018 at 1188.404 kb on - strand, within Pf6N2E2_1018 at 1188.404 kb on - strand, within Pf6N2E2_1018 at 1188.404 kb on - strand, within Pf6N2E2_1018 at 1188.404 kb on - strand, within Pf6N2E2_1018 at 1188.406 kb on + strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.434 kb on - strand, within Pf6N2E2_1018 at 1188.519 kb on + strand, within Pf6N2E2_1018 at 1188.520 kb on - strand, within Pf6N2E2_1018 at 1188.561 kb on + strand, within Pf6N2E2_1018 at 1188.561 kb on + strand, within Pf6N2E2_1018 at 1188.789 kb on + strand, within Pf6N2E2_1018 at 1188.789 kb on + strand, within Pf6N2E2_1018 at 1188.790 kb on - strand, within Pf6N2E2_1018 at 1188.968 kb on + strand at 1189.008 kb on - strand at 1189.008 kb on - strand at 1189.050 kb on + strand at 1189.131 kb on - strand at 1189.131 kb on - strand at 1189.167 kb on - strand at 1189.172 kb on + strand at 1189.225 kb on - strand at 1189.228 kb on + strand at 1189.229 kb on - strand at 1189.246 kb on - strand at 1189.253 kb on + strand at 1189.253 kb on + strand at 1189.254 kb on - strand at 1189.304 kb on - strand at 1189.382 kb on + strand at 1189.405 kb on + strand at 1189.418 kb on + strand at 1189.419 kb on - strand at 1189.419 kb on - strand at 1189.422 kb on + strand at 1189.422 kb on + strand at 1189.422 kb on + strand at 1189.422 kb on + strand at 1189.422 kb on + strand at 1189.423 kb on - strand at 1189.428 kb on + strand at 1189.428 kb on + strand at 1189.428 kb on + strand at 1189.428 kb on + strand at 1189.429 kb on - strand at 1189.478 kb on - strand at 1189.560 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.640 kb on - strand at 1189.640 kb on - strand at 1189.640 kb on - strand at 1189.723 kb on + strand at 1189.806 kb on + strand at 1189.806 kb on + strand at 1189.907 kb on + strand at 1189.907 kb on + strand at 1189.907 kb on + strand at 1189.907 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C remove 1,187,430 + Pf6N2E2_1017 0.44 +1.0 1,187,865 + -0.8 1,187,865 + +0.1 1,187,885 - -0.1 1,187,921 + -0.5 1,187,921 + +0.2 1,187,921 + +0.2 1,187,921 + +0.1 1,187,921 + -0.5 1,187,921 + -0.5 1,187,921 + +0.3 1,187,921 + +1.5 1,187,922 - +0.3 1,187,968 + +1.0 1,187,969 - +1.5 1,188,112 + -0.4 1,188,113 - +1.5 1,188,113 - -0.4 1,188,135 + Pf6N2E2_1018 0.10 +0.2 1,188,212 + Pf6N2E2_1018 0.18 +0.6 1,188,212 + Pf6N2E2_1018 0.18 +0.8 1,188,213 - Pf6N2E2_1018 0.18 +1.2 1,188,213 - Pf6N2E2_1018 0.18 +0.1 1,188,213 - Pf6N2E2_1018 0.18 +0.5 1,188,404 - Pf6N2E2_1018 0.36 +1.5 1,188,404 - Pf6N2E2_1018 0.36 +0.5 1,188,404 - Pf6N2E2_1018 0.36 +0.9 1,188,404 - Pf6N2E2_1018 0.36 +1.4 1,188,406 + Pf6N2E2_1018 0.37 -0.5 1,188,407 - Pf6N2E2_1018 0.37 -0.9 1,188,407 - Pf6N2E2_1018 0.37 -1.8 1,188,407 - Pf6N2E2_1018 0.37 +0.3 1,188,407 - Pf6N2E2_1018 0.37 -0.5 1,188,407 - Pf6N2E2_1018 0.37 +1.5 1,188,434 - Pf6N2E2_1018 0.39 +0.5 1,188,519 + Pf6N2E2_1018 0.48 +3.1 1,188,520 - Pf6N2E2_1018 0.48 +2.1 1,188,561 + Pf6N2E2_1018 0.52 +0.3 1,188,561 + Pf6N2E2_1018 0.52 +1.5 1,188,789 + Pf6N2E2_1018 0.74 +1.3 1,188,789 + Pf6N2E2_1018 0.74 +1.5 1,188,790 - Pf6N2E2_1018 0.74 +0.7 1,188,968 + +1.6 1,189,008 - +2.5 1,189,008 - +1.1 1,189,050 + -0.9 1,189,131 - +0.2 1,189,131 - -0.5 1,189,167 - +1.6 1,189,172 + +0.1 1,189,225 - +0.9 1,189,228 + +0.1 1,189,229 - -1.3 1,189,246 - +1.7 1,189,253 + +1.0 1,189,253 + +0.5 1,189,254 - +1.9 1,189,304 - +0.4 1,189,382 + +1.5 1,189,405 + +0.5 1,189,418 + -1.8 1,189,419 - +1.5 1,189,419 - +1.5 1,189,422 + +0.5 1,189,422 + +0.2 1,189,422 + -0.3 1,189,422 + -0.4 1,189,422 + -0.2 1,189,423 - -0.1 1,189,428 + -1.6 1,189,428 + -2.8 1,189,428 + +2.5 1,189,428 + +0.7 1,189,429 - +2.5 1,189,478 - -0.5 1,189,560 + -1.1 1,189,639 + -0.1 1,189,639 + +0.7 1,189,639 + -0.7 1,189,639 + +1.1 1,189,639 + -0.3 1,189,639 + -0.8 1,189,640 - +0.5 1,189,640 - -1.3 1,189,640 - -0.2 1,189,723 + -0.2 1,189,806 + -1.0 1,189,806 + +1.0 1,189,907 + +0.9 1,189,907 + -0.9 1,189,907 + -0.7 1,189,907 + +1.1
Or see this region's nucleotide sequence