Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1403 and SMa1406 are separated by 114 nucleotides SMa1406 and SMa1408 are separated by 12 nucleotides SMa1408 and SMa1409 overlap by 4 nucleotides
SMa1403: SMa1403 - oxidoreductase, at 771,034 to 772,050
SMa1403
SMa1406: SMa1406 - TtuD3 hydroxypyruvate reductase, at 772,165 to 773,424
SMa1406
SMa1408: SMa1408 - dehydratase, at 773,437 to 774,207
SMa1408
SMa1409: SMa1409 - acyl-CoA transferase, at 774,204 to 775,775
SMa1409
Position (kb) 772
773
774 Strain fitness (log2 ratio) -2
-1
0
1
2 at 771.208 kb on + strand, within SMa1403 at 771.208 kb on + strand, within SMa1403 at 771.209 kb on - strand, within SMa1403 at 771.271 kb on + strand, within SMa1403 at 771.272 kb on - strand, within SMa1403 at 771.272 kb on - strand, within SMa1403 at 771.376 kb on + strand, within SMa1403 at 771.382 kb on + strand, within SMa1403 at 771.382 kb on + strand, within SMa1403 at 771.382 kb on + strand, within SMa1403 at 771.382 kb on + strand, within SMa1403 at 771.501 kb on - strand, within SMa1403 at 771.656 kb on - strand, within SMa1403 at 771.657 kb on + strand, within SMa1403 at 771.657 kb on + strand, within SMa1403 at 771.658 kb on - strand at 771.658 kb on - strand, within SMa1403 at 771.790 kb on + strand, within SMa1403 at 771.790 kb on + strand, within SMa1403 at 771.791 kb on - strand, within SMa1403 at 771.791 kb on - strand, within SMa1403 at 771.791 kb on - strand, within SMa1403 at 771.813 kb on - strand, within SMa1403 at 771.813 kb on - strand, within SMa1403 at 772.151 kb on - strand at 772.437 kb on + strand, within SMa1406 at 772.674 kb on + strand, within SMa1406 at 773.188 kb on + strand, within SMa1406 at 773.210 kb on + strand, within SMa1406 at 773.210 kb on + strand, within SMa1406 at 773.216 kb on + strand, within SMa1406 at 773.583 kb on + strand, within SMa1408 at 773.583 kb on + strand, within SMa1408 at 773.615 kb on - strand, within SMa1408 at 773.615 kb on - strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.795 kb on - strand, within SMa1408 at 773.821 kb on + strand, within SMa1408 at 773.821 kb on + strand, within SMa1408 at 773.885 kb on - strand, within SMa1408 at 774.056 kb on - strand, within SMa1408 at 774.056 kb on - strand, within SMa1408 at 774.142 kb on - strand at 774.142 kb on - strand at 774.213 kb on + strand at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 771,208 + SMa1403 0.17 +0.4 771,208 + SMa1403 0.17 -2.5 771,209 - SMa1403 0.17 +0.3 771,271 + SMa1403 0.23 -0.3 771,272 - SMa1403 0.23 -1.5 771,272 - SMa1403 0.23 +0.3 771,376 + SMa1403 0.34 -0.4 771,382 + SMa1403 0.34 +0.2 771,382 + SMa1403 0.34 -0.1 771,382 + SMa1403 0.34 +0.8 771,382 + SMa1403 0.34 -0.9 771,501 - SMa1403 0.46 +1.4 771,656 - SMa1403 0.61 +0.6 771,657 + SMa1403 0.61 +0.4 771,657 + SMa1403 0.61 -1.6 771,658 - -2.0 771,658 - SMa1403 0.61 -0.8 771,790 + SMa1403 0.74 -0.8 771,790 + SMa1403 0.74 -2.7 771,791 - SMa1403 0.74 +2.2 771,791 - SMa1403 0.74 -1.8 771,791 - SMa1403 0.74 +1.3 771,813 - SMa1403 0.77 +0.3 771,813 - SMa1403 0.77 -0.2 772,151 - -1.7 772,437 + SMa1406 0.22 -0.4 772,674 + SMa1406 0.40 -0.3 773,188 + SMa1406 0.81 -1.1 773,210 + SMa1406 0.83 -0.2 773,210 + SMa1406 0.83 -1.0 773,216 + SMa1406 0.83 -0.1 773,583 + SMa1408 0.19 +1.8 773,583 + SMa1408 0.19 -0.2 773,615 - SMa1408 0.23 -1.5 773,615 - SMa1408 0.23 +0.1 773,708 + SMa1408 0.35 +0.3 773,708 + SMa1408 0.35 +0.2 773,708 + SMa1408 0.35 -0.1 773,795 - SMa1408 0.46 -0.7 773,821 + SMa1408 0.50 +0.9 773,821 + SMa1408 0.50 -0.2 773,885 - SMa1408 0.58 -0.4 774,056 - SMa1408 0.80 +0.3 774,056 - SMa1408 0.80 -0.3 774,142 - +0.0 774,142 - -1.8 774,213 + +0.0 774,388 + SMa1409 0.12 -0.1 774,388 + SMa1409 0.12 -1.8 774,388 + SMa1409 0.12 -1.1 774,388 + SMa1409 0.12 +0.0 774,389 - SMa1409 0.12 -2.6 774,389 - SMa1409 0.12 -0.1 774,389 - SMa1409 0.12 +2.3 774,389 - SMa1409 0.12 +0.2
Or see this region's nucleotide sequence