Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21428 and SM_b21429 are separated by 24 nucleotides SM_b21429 and SM_b21430 overlap by 7 nucleotides SM_b21430 and SM_b21431 are separated by 126 nucleotides SM_b21431 and SM_b21432 are separated by 2 nucleotides
SM_b21428: SM_b21428 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 1,359,270 to 1,359,833
_b21428
SM_b21429: SM_b21429 - iron ABC transporter ATP-binding protein, at 1,359,858 to 1,360,619
_b21429
SM_b21430: SM_b21430 - iron ABC transporter permease, at 1,360,613 to 1,361,683
_b21430
SM_b21431: SM_b21431 - hypothetical protein, at 1,361,810 to 1,362,232
_b21431
SM_b21432: SM_b21432 - iron uptake ABC transporter substrate-binding protein precursor, at 1,362,235 to 1,362,942
_b21432
Position (kb)
1360
1361
1362 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1359.938 kb on - strand, within SM_b21429 at 1360.030 kb on + strand, within SM_b21429 at 1360.030 kb on + strand, within SM_b21429 at 1360.228 kb on - strand, within SM_b21429 at 1360.358 kb on - strand, within SM_b21429 at 1360.399 kb on + strand, within SM_b21429 at 1360.399 kb on + strand, within SM_b21429 at 1360.399 kb on + strand, within SM_b21429 at 1360.400 kb on - strand, within SM_b21429 at 1360.400 kb on - strand, within SM_b21429 at 1360.400 kb on - strand, within SM_b21429 at 1360.435 kb on - strand, within SM_b21429 at 1360.440 kb on + strand, within SM_b21429 at 1360.441 kb on - strand, within SM_b21429 at 1360.582 kb on + strand at 1360.583 kb on - strand at 1360.614 kb on + strand at 1360.807 kb on + strand, within SM_b21430 at 1360.807 kb on + strand, within SM_b21430 at 1360.808 kb on - strand, within SM_b21430 at 1360.816 kb on + strand, within SM_b21430 at 1360.946 kb on - strand, within SM_b21430 at 1361.263 kb on + strand, within SM_b21430 at 1361.263 kb on + strand, within SM_b21430 at 1361.263 kb on + strand, within SM_b21430 at 1361.263 kb on + strand, within SM_b21430 at 1361.264 kb on - strand, within SM_b21430 at 1361.264 kb on - strand, within SM_b21430 at 1361.264 kb on - strand, within SM_b21430 at 1361.266 kb on - strand, within SM_b21430 at 1361.453 kb on + strand, within SM_b21430 at 1361.453 kb on + strand, within SM_b21430 at 1361.453 kb on + strand, within SM_b21430 at 1361.453 kb on + strand, within SM_b21430 at 1361.453 kb on + strand, within SM_b21430 at 1361.454 kb on - strand, within SM_b21430 at 1361.454 kb on - strand, within SM_b21430 at 1361.533 kb on - strand, within SM_b21430 at 1361.533 kb on - strand, within SM_b21430 at 1361.632 kb on - strand at 1361.750 kb on - strand at 1361.758 kb on + strand at 1361.758 kb on + strand at 1361.759 kb on - strand at 1361.759 kb on - strand at 1361.839 kb on - strand at 1361.851 kb on - strand at 1361.964 kb on + strand, within SM_b21431 at 1361.976 kb on + strand, within SM_b21431 at 1361.977 kb on - strand, within SM_b21431 at 1362.093 kb on + strand, within SM_b21431 at 1362.126 kb on + strand, within SM_b21431 at 1362.126 kb on + strand, within SM_b21431 at 1362.126 kb on + strand, within SM_b21431 at 1362.126 kb on + strand, within SM_b21431 at 1362.126 kb on + strand, within SM_b21431 at 1362.126 kb on + strand, within SM_b21431 at 1362.127 kb on - strand, within SM_b21431 at 1362.127 kb on - strand, within SM_b21431 at 1362.127 kb on - strand, within SM_b21431 at 1362.151 kb on + strand, within SM_b21431 at 1362.151 kb on + strand, within SM_b21431 at 1362.498 kb on - strand, within SM_b21432 at 1362.535 kb on - strand, within SM_b21432 at 1362.536 kb on + strand, within SM_b21432 at 1362.537 kb on - strand at 1362.537 kb on - strand, within SM_b21432
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 1,359,938 - SM_b21429 0.10 +3.1 1,360,030 + SM_b21429 0.23 +0.3 1,360,030 + SM_b21429 0.23 -0.0 1,360,228 - SM_b21429 0.49 +0.8 1,360,358 - SM_b21429 0.66 -0.7 1,360,399 + SM_b21429 0.71 -0.7 1,360,399 + SM_b21429 0.71 -3.1 1,360,399 + SM_b21429 0.71 +1.6 1,360,400 - SM_b21429 0.71 -1.9 1,360,400 - SM_b21429 0.71 +2.3 1,360,400 - SM_b21429 0.71 +0.4 1,360,435 - SM_b21429 0.76 +0.5 1,360,440 + SM_b21429 0.76 -0.1 1,360,441 - SM_b21429 0.77 +0.3 1,360,582 + -0.3 1,360,583 - +1.9 1,360,614 + +0.4 1,360,807 + SM_b21430 0.18 +0.8 1,360,807 + SM_b21430 0.18 -0.2 1,360,808 - SM_b21430 0.18 +1.1 1,360,816 + SM_b21430 0.19 +1.9 1,360,946 - SM_b21430 0.31 -0.7 1,361,263 + SM_b21430 0.61 +0.9 1,361,263 + SM_b21430 0.61 +1.8 1,361,263 + SM_b21430 0.61 +0.7 1,361,263 + SM_b21430 0.61 +1.5 1,361,264 - SM_b21430 0.61 -0.9 1,361,264 - SM_b21430 0.61 -2.0 1,361,264 - SM_b21430 0.61 +1.4 1,361,266 - SM_b21430 0.61 -0.2 1,361,453 + SM_b21430 0.78 -0.8 1,361,453 + SM_b21430 0.78 -0.5 1,361,453 + SM_b21430 0.78 +0.4 1,361,453 + SM_b21430 0.78 -2.8 1,361,453 + SM_b21430 0.78 -0.2 1,361,454 - SM_b21430 0.79 +0.2 1,361,454 - SM_b21430 0.79 +1.1 1,361,533 - SM_b21430 0.86 +0.9 1,361,533 - SM_b21430 0.86 +0.9 1,361,632 - +0.4 1,361,750 - -0.1 1,361,758 + +0.3 1,361,758 + -1.4 1,361,759 - +0.7 1,361,759 - -1.0 1,361,839 - +0.7 1,361,851 - +1.7 1,361,964 + SM_b21431 0.36 +0.6 1,361,976 + SM_b21431 0.39 +0.6 1,361,977 - SM_b21431 0.39 +0.4 1,362,093 + SM_b21431 0.67 +1.4 1,362,126 + SM_b21431 0.75 -0.2 1,362,126 + SM_b21431 0.75 -1.0 1,362,126 + SM_b21431 0.75 +0.5 1,362,126 + SM_b21431 0.75 +0.4 1,362,126 + SM_b21431 0.75 +0.0 1,362,126 + SM_b21431 0.75 -0.5 1,362,127 - SM_b21431 0.75 -1.9 1,362,127 - SM_b21431 0.75 +1.4 1,362,127 - SM_b21431 0.75 -2.8 1,362,151 + SM_b21431 0.81 -0.3 1,362,151 + SM_b21431 0.81 -0.5 1,362,498 - SM_b21432 0.37 +2.2 1,362,535 - SM_b21432 0.42 -1.2 1,362,536 + SM_b21432 0.43 +1.1 1,362,537 - +1.8 1,362,537 - SM_b21432 0.43 +1.7
Or see this region's nucleotide sequence