Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21224 and SM_b21225 overlap by 4 nucleotides SM_b21225 and SM_b21226 are separated by 46 nucleotides SM_b21226 and SM_b21227 overlap by 4 nucleotides SM_b21227 and SM_b21228 are separated by 5 nucleotides
SM_b21224: SM_b21224 - bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase, at 787,536 to 789,437
_b21224
SM_b21225: SM_b21225 - inositol monophosphatase, at 789,434 to 790,273
_b21225
SM_b21226: SM_b21226 - hypothetical protein, at 790,320 to 791,060
_b21226
SM_b21227: SM_b21227 - carbamoyl phosphate synthase-like protein, at 791,057 to 792,049
_b21227
SM_b21228: SM_b21228 - nucleotide sugar epimerase dehydratase, at 792,055 to 792,990
_b21228
Position (kb) 790
791
792 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 789.356 kb on + strand at 789.356 kb on + strand at 789.357 kb on - strand at 789.357 kb on - strand at 789.358 kb on + strand at 789.359 kb on - strand at 789.359 kb on - strand at 789.359 kb on - strand at 789.359 kb on - strand at 789.500 kb on + strand at 789.500 kb on + strand at 789.809 kb on + strand, within SM_b21225 at 789.810 kb on - strand, within SM_b21225 at 789.984 kb on - strand, within SM_b21225 at 790.043 kb on + strand, within SM_b21225 at 790.044 kb on - strand, within SM_b21225 at 790.259 kb on + strand at 790.260 kb on - strand at 790.339 kb on + strand at 790.339 kb on + strand at 790.340 kb on - strand at 790.340 kb on - strand at 790.507 kb on + strand, within SM_b21226 at 790.507 kb on + strand, within SM_b21226 at 790.508 kb on - strand, within SM_b21226 at 790.578 kb on - strand, within SM_b21226 at 790.578 kb on - strand, within SM_b21226 at 790.588 kb on - strand, within SM_b21226 at 790.857 kb on + strand, within SM_b21226 at 791.033 kb on - strand at 791.033 kb on - strand at 791.068 kb on + strand at 791.068 kb on + strand at 791.069 kb on - strand at 791.143 kb on - strand at 791.180 kb on + strand, within SM_b21227 at 791.198 kb on + strand, within SM_b21227 at 791.199 kb on - strand, within SM_b21227 at 791.229 kb on - strand, within SM_b21227 at 791.342 kb on + strand, within SM_b21227 at 791.343 kb on - strand at 791.439 kb on - strand, within SM_b21227 at 791.633 kb on + strand, within SM_b21227 at 791.633 kb on + strand, within SM_b21227 at 791.710 kb on + strand, within SM_b21227 at 791.710 kb on + strand, within SM_b21227 at 791.711 kb on - strand, within SM_b21227 at 791.714 kb on + strand, within SM_b21227 at 791.714 kb on + strand, within SM_b21227 at 791.715 kb on - strand, within SM_b21227 at 791.715 kb on - strand, within SM_b21227 at 791.786 kb on + strand, within SM_b21227 at 791.787 kb on - strand, within SM_b21227 at 791.787 kb on - strand, within SM_b21227 at 791.970 kb on + strand at 791.971 kb on - strand at 791.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 789,356 + -0.2 789,356 + +0.7 789,357 - +0.4 789,357 - -0.2 789,358 + +0.2 789,359 - +1.0 789,359 - -1.8 789,359 - +0.4 789,359 - -0.0 789,500 + -3.1 789,500 + -2.5 789,809 + SM_b21225 0.45 -0.9 789,810 - SM_b21225 0.45 -0.7 789,984 - SM_b21225 0.65 -0.6 790,043 + SM_b21225 0.72 +0.8 790,044 - SM_b21225 0.73 -1.0 790,259 + -0.3 790,260 - -1.6 790,339 + -2.8 790,339 + +0.0 790,340 - -0.3 790,340 - -2.1 790,507 + SM_b21226 0.25 -1.1 790,507 + SM_b21226 0.25 -2.4 790,508 - SM_b21226 0.25 -2.5 790,578 - SM_b21226 0.35 -3.2 790,578 - SM_b21226 0.35 -0.5 790,588 - SM_b21226 0.36 +0.4 790,857 + SM_b21226 0.72 -1.1 791,033 - +0.8 791,033 - -0.6 791,068 + -0.5 791,068 + +3.1 791,069 - -0.2 791,143 - -1.1 791,180 + SM_b21227 0.12 +1.8 791,198 + SM_b21227 0.14 -1.9 791,199 - SM_b21227 0.14 -3.2 791,229 - SM_b21227 0.17 +0.4 791,342 + SM_b21227 0.29 +0.5 791,343 - -0.9 791,439 - SM_b21227 0.38 -0.9 791,633 + SM_b21227 0.58 -0.3 791,633 + SM_b21227 0.58 -0.2 791,710 + SM_b21227 0.66 -0.5 791,710 + SM_b21227 0.66 -1.2 791,711 - SM_b21227 0.66 +0.8 791,714 + SM_b21227 0.66 -0.0 791,714 + SM_b21227 0.66 -2.1 791,715 - SM_b21227 0.66 -0.0 791,715 - SM_b21227 0.66 +1.4 791,786 + SM_b21227 0.73 +0.3 791,787 - SM_b21227 0.74 +0.3 791,787 - SM_b21227 0.74 +1.8 791,970 + +0.5 791,971 - +0.9 791,971 - -1.0
Or see this region's nucleotide sequence