Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_695 and Pf6N2E2_696 overlap by 4 nucleotides Pf6N2E2_696 and Pf6N2E2_697 are separated by 40 nucleotides
Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011
_695
Pf6N2E2_696 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 803,008 to 803,850
_696
Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069
_697
Position (kb) 803
804 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 802.124 kb on - strand, within Pf6N2E2_695 at 802.224 kb on + strand, within Pf6N2E2_695 at 802.432 kb on - strand, within Pf6N2E2_695 at 802.726 kb on - strand, within Pf6N2E2_695 at 802.917 kb on + strand at 802.917 kb on + strand at 802.917 kb on + strand at 802.917 kb on + strand at 802.918 kb on - strand at 802.918 kb on - strand at 802.982 kb on + strand at 802.982 kb on + strand at 802.983 kb on - strand at 802.985 kb on + strand at 802.985 kb on + strand at 802.985 kb on + strand at 802.986 kb on - strand at 802.986 kb on - strand at 802.986 kb on - strand at 803.232 kb on + strand, within Pf6N2E2_696 at 803.232 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.429 kb on + strand, within Pf6N2E2_696 at 803.429 kb on + strand, within Pf6N2E2_696 at 803.430 kb on - strand, within Pf6N2E2_696 at 803.430 kb on - strand, within Pf6N2E2_696 at 803.499 kb on - strand, within Pf6N2E2_696 at 803.646 kb on - strand, within Pf6N2E2_696 at 803.705 kb on + strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.191 kb on - strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.486 kb on - strand, within Pf6N2E2_697 at 804.493 kb on - strand, within Pf6N2E2_697 at 804.681 kb on + strand, within Pf6N2E2_697 at 804.682 kb on - strand, within Pf6N2E2_697 at 804.682 kb on - strand, within Pf6N2E2_697 at 804.777 kb on + strand, within Pf6N2E2_697
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 802,124 - Pf6N2E2_695 0.41 -0.9 802,224 + Pf6N2E2_695 0.47 -2.0 802,432 - Pf6N2E2_695 0.61 -1.1 802,726 - Pf6N2E2_695 0.81 +0.1 802,917 + -3.2 802,917 + -1.1 802,917 + +0.1 802,917 + +1.6 802,918 - +0.1 802,918 - -0.5 802,982 + -1.9 802,982 + -0.7 802,983 - -0.3 802,985 + -0.1 802,985 + -1.0 802,985 + -0.5 802,986 - +0.9 802,986 - -2.0 802,986 - -0.7 803,232 + Pf6N2E2_696 0.27 -0.9 803,232 + Pf6N2E2_696 0.27 -1.4 803,297 + Pf6N2E2_696 0.34 -0.8 803,297 + Pf6N2E2_696 0.34 -0.7 803,297 + Pf6N2E2_696 0.34 -1.4 803,297 + Pf6N2E2_696 0.34 -0.7 803,429 + Pf6N2E2_696 0.50 -2.7 803,429 + Pf6N2E2_696 0.50 -0.6 803,430 - Pf6N2E2_696 0.50 -0.0 803,430 - Pf6N2E2_696 0.50 -0.5 803,499 - Pf6N2E2_696 0.58 +0.0 803,646 - Pf6N2E2_696 0.76 -0.4 803,705 + Pf6N2E2_696 0.83 +0.0 803,706 - Pf6N2E2_696 0.83 -0.6 803,706 - Pf6N2E2_696 0.83 -0.5 803,706 - Pf6N2E2_696 0.83 -1.1 804,165 + Pf6N2E2_697 0.23 -2.1 804,165 + Pf6N2E2_697 0.23 +0.3 804,165 + Pf6N2E2_697 0.23 -0.6 804,165 + Pf6N2E2_697 0.23 +0.1 804,191 - Pf6N2E2_697 0.25 +0.6 804,456 + Pf6N2E2_697 0.48 -0.2 804,456 + Pf6N2E2_697 0.48 -0.4 804,456 + Pf6N2E2_697 0.48 -0.1 804,457 - Pf6N2E2_697 0.48 +0.2 804,457 - Pf6N2E2_697 0.48 -0.6 804,457 - Pf6N2E2_697 0.48 -0.0 804,457 - Pf6N2E2_697 0.48 -0.0 804,486 - Pf6N2E2_697 0.50 -0.8 804,493 - Pf6N2E2_697 0.51 -0.2 804,681 + Pf6N2E2_697 0.67 +1.4 804,682 - Pf6N2E2_697 0.67 -0.2 804,682 - Pf6N2E2_697 0.67 -1.6 804,777 + Pf6N2E2_697 0.75 -0.2
Or see this region's nucleotide sequence