Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5933 and Pf6N2E2_5934 are separated by 23 nucleotides Pf6N2E2_5934 and Pf6N2E2_5935 overlap by 4 nucleotides Pf6N2E2_5935 and Pf6N2E2_5936 overlap by 1 nucleotides Pf6N2E2_5936 and Pf6N2E2_5937 overlap by 4 nucleotides
Pf6N2E2_5933 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 6,753,413 to 6,754,423
_5933
Pf6N2E2_5934 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 6,754,447 to 6,754,632
_5934
Pf6N2E2_5935 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 6,754,629 to 6,755,393
_5935
Pf6N2E2_5936 - Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48), at 6,755,393 to 6,755,857
_5936
Pf6N2E2_5937 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 6,755,854 to 6,756,873
_5937
Position (kb)
6755
6756 Strain fitness (log2 ratio)
-1
0
1 at 6754.421 kb on + strand at 6754.431 kb on + strand at 6755.422 kb on + strand at 6755.633 kb on + strand, within Pf6N2E2_5936 at 6755.765 kb on + strand, within Pf6N2E2_5936
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 6,754,421 + +0.1 6,754,431 + -1.4 6,755,422 + +0.5 6,755,633 + Pf6N2E2_5936 0.52 -0.1 6,755,765 + Pf6N2E2_5936 0.80 +0.2
Or see this region's nucleotide sequence