Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_552

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_551 and Pf6N2E2_552 overlap by 17 nucleotidesPf6N2E2_552 and Pf6N2E2_553 are separated by 13 nucleotidesPf6N2E2_553 and Pf6N2E2_554 are separated by 197 nucleotides Pf6N2E2_551 - Protein ImpG/VasA, at 622,878 to 624,668 _551 Pf6N2E2_552 - Uncharacterized protein ImpF, at 624,652 to 625,095 _552 Pf6N2E2_553 - Uncharacterized protein ImpD, at 625,109 to 625,606 _553 Pf6N2E2_554 - Uncharacterized protein ImpC, at 625,804 to 627,285 _554 Position (kb) 624 625 626Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 623.815 kb on + strand, within Pf6N2E2_551at 623.815 kb on + strand, within Pf6N2E2_551at 623.815 kb on + strand, within Pf6N2E2_551at 623.821 kb on + strand, within Pf6N2E2_551at 623.821 kb on + strand, within Pf6N2E2_551at 623.821 kb on + strand, within Pf6N2E2_551at 623.896 kb on + strand, within Pf6N2E2_551at 623.897 kb on - strand, within Pf6N2E2_551at 623.925 kb on + strand, within Pf6N2E2_551at 623.925 kb on + strand, within Pf6N2E2_551at 623.926 kb on - strand, within Pf6N2E2_551at 624.455 kb on - strand, within Pf6N2E2_551at 624.631 kb on + strandat 624.631 kb on + strandat 624.631 kb on + strandat 624.631 kb on + strandat 624.632 kb on - strandat 624.635 kb on - strandat 624.674 kb on - strandat 624.757 kb on + strand, within Pf6N2E2_552at 624.758 kb on - strand, within Pf6N2E2_552at 624.917 kb on + strand, within Pf6N2E2_552at 625.110 kb on + strandat 625.296 kb on + strand, within Pf6N2E2_553at 625.297 kb on - strand, within Pf6N2E2_553at 625.297 kb on - strand, within Pf6N2E2_553at 625.297 kb on - strand, within Pf6N2E2_553at 625.389 kb on + strand, within Pf6N2E2_553at 625.518 kb on + strand, within Pf6N2E2_553at 625.518 kb on + strand, within Pf6N2E2_553at 625.519 kb on - strand, within Pf6N2E2_553at 625.633 kb on + strandat 625.633 kb on + strandat 625.633 kb on + strandat 625.633 kb on + strandat 625.633 kb on + strandat 625.634 kb on - strandat 625.634 kb on - strandat 625.634 kb on - strandat 625.634 kb on - strandat 625.634 kb on - strandat 625.634 kb on - strandat 625.654 kb on - strandat 625.654 kb on - strandat 625.767 kb on + strandat 625.767 kb on + strandat 625.767 kb on + strandat 625.768 kb on - strandat 625.775 kb on + strandat 625.776 kb on - strandat 625.783 kb on + strandat 625.783 kb on + strandat 625.783 kb on + strandat 625.783 kb on + strandat 625.783 kb on + strandat 625.784 kb on - strandat 625.784 kb on - strandat 625.791 kb on + strandat 625.791 kb on + strandat 625.814 kb on - strandat 625.902 kb on - strandat 625.902 kb on - strandat 626.003 kb on + strand, within Pf6N2E2_554at 626.027 kb on + strand, within Pf6N2E2_554at 626.027 kb on + strand, within Pf6N2E2_554at 626.028 kb on - strand, within Pf6N2E2_554at 626.035 kb on + strand, within Pf6N2E2_554at 626.035 kb on + strand, within Pf6N2E2_554at 626.035 kb on + strand, within Pf6N2E2_554at 626.035 kb on + strand, within Pf6N2E2_554at 626.035 kb on + strand, within Pf6N2E2_554at 626.036 kb on - strand, within Pf6N2E2_554at 626.036 kb on - strand, within Pf6N2E2_554at 626.036 kb on - strand, within Pf6N2E2_554at 626.036 kb on - strand, within Pf6N2E2_554at 626.036 kb on - strand, within Pf6N2E2_554at 626.036 kb on - strand, within Pf6N2E2_554at 626.090 kb on + strand, within Pf6N2E2_554

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
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623,815 + Pf6N2E2_551 0.52 -2.3
623,815 + Pf6N2E2_551 0.52 -0.7
623,815 + Pf6N2E2_551 0.52 -1.2
623,821 + Pf6N2E2_551 0.53 -0.0
623,821 + Pf6N2E2_551 0.53 +1.4
623,821 + Pf6N2E2_551 0.53 -0.5
623,896 + Pf6N2E2_551 0.57 +0.3
623,897 - Pf6N2E2_551 0.57 -0.4
623,925 + Pf6N2E2_551 0.58 +0.2
623,925 + Pf6N2E2_551 0.58 -0.1
623,926 - Pf6N2E2_551 0.59 +2.4
624,455 - Pf6N2E2_551 0.88 -0.3
624,631 + +0.6
624,631 + -0.5
624,631 + -0.6
624,631 + -0.9
624,632 - -0.5
624,635 - -1.0
624,674 - +1.5
624,757 + Pf6N2E2_552 0.24 -2.9
624,758 - Pf6N2E2_552 0.24 -0.4
624,917 + Pf6N2E2_552 0.60 -2.4
625,110 + -1.1
625,296 + Pf6N2E2_553 0.38 -0.5
625,297 - Pf6N2E2_553 0.38 +1.0
625,297 - Pf6N2E2_553 0.38 +0.1
625,297 - Pf6N2E2_553 0.38 +0.1
625,389 + Pf6N2E2_553 0.56 -0.2
625,518 + Pf6N2E2_553 0.82 -0.9
625,518 + Pf6N2E2_553 0.82 -0.4
625,519 - Pf6N2E2_553 0.82 +0.2
625,633 + -0.6
625,633 + -0.2
625,633 + -1.2
625,633 + -0.0
625,633 + +0.0
625,634 - +0.1
625,634 - -0.9
625,634 - -0.6
625,634 - +0.4
625,634 - +0.4
625,634 - +0.4
625,654 - +1.9
625,654 - +0.1
625,767 + +0.6
625,767 + +0.2
625,767 + -0.2
625,768 - +1.4
625,775 + +0.1
625,776 - -0.1
625,783 + +2.1
625,783 + -0.2
625,783 + -0.7
625,783 + +0.1
625,783 + +0.0
625,784 - +1.7
625,784 - -0.6
625,791 + +0.8
625,791 + -0.2
625,814 - +0.4
625,902 - -0.8
625,902 - +0.2
626,003 + Pf6N2E2_554 0.13 -0.3
626,027 + Pf6N2E2_554 0.15 -1.4
626,027 + Pf6N2E2_554 0.15 -0.1
626,028 - Pf6N2E2_554 0.15 -0.6
626,035 + Pf6N2E2_554 0.16 -0.6
626,035 + Pf6N2E2_554 0.16 -0.3
626,035 + Pf6N2E2_554 0.16 +1.4
626,035 + Pf6N2E2_554 0.16 -1.0
626,035 + Pf6N2E2_554 0.16 -0.6
626,036 - Pf6N2E2_554 0.16 +0.2
626,036 - Pf6N2E2_554 0.16 +1.1
626,036 - Pf6N2E2_554 0.16 +1.4
626,036 - Pf6N2E2_554 0.16 +0.6
626,036 - Pf6N2E2_554 0.16 -0.3
626,036 - Pf6N2E2_554 0.16 -0.3
626,090 + Pf6N2E2_554 0.19 -0.3

Or see this region's nucleotide sequence