Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5395 and Pf6N2E2_5396 are separated by 128 nucleotides Pf6N2E2_5396 and Pf6N2E2_5397 are separated by 89 nucleotides Pf6N2E2_5397 and Pf6N2E2_5398 are separated by 232 nucleotides Pf6N2E2_5398 and Pf6N2E2_5399 are separated by 1 nucleotides
Pf6N2E2_5395 - Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein, at 6,200,942 to 6,201,676
_5395
Pf6N2E2_5396 - YheO-like PAS domain, at 6,201,805 to 6,202,431
_5396
Pf6N2E2_5397 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 6,202,521 to 6,203,477
_5397
Pf6N2E2_5398 - Ornithine cyclodeaminase (EC 4.3.1.12), at 6,203,710 to 6,203,892
_5398
Pf6N2E2_5399 - Ornithine cyclodeaminase (EC 4.3.1.12), at 6,203,894 to 6,204,661
_5399
Position (kb)
6202
6203
6204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6201.527 kb on + strand, within Pf6N2E2_5395 at 6201.528 kb on - strand, within Pf6N2E2_5395 at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.665 kb on + strand at 6201.665 kb on + strand at 6201.666 kb on - strand at 6201.677 kb on + strand at 6201.677 kb on + strand at 6201.678 kb on - strand at 6201.678 kb on - strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.746 kb on + strand at 6201.747 kb on - strand at 6201.833 kb on + strand at 6201.833 kb on + strand at 6201.833 kb on + strand at 6201.834 kb on - strand at 6201.834 kb on - strand at 6201.852 kb on + strand at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6202.110 kb on + strand, within Pf6N2E2_5396 at 6202.111 kb on - strand, within Pf6N2E2_5396 at 6202.162 kb on + strand, within Pf6N2E2_5396 at 6202.163 kb on - strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.297 kb on - strand, within Pf6N2E2_5396 at 6202.307 kb on + strand, within Pf6N2E2_5396 at 6202.307 kb on + strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.358 kb on + strand, within Pf6N2E2_5396 at 6202.358 kb on + strand, within Pf6N2E2_5396 at 6202.722 kb on + strand, within Pf6N2E2_5397 at 6202.723 kb on - strand, within Pf6N2E2_5397 at 6202.795 kb on + strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6203.178 kb on - strand, within Pf6N2E2_5397 at 6203.178 kb on - strand, within Pf6N2E2_5397 at 6203.188 kb on + strand, within Pf6N2E2_5397 at 6203.188 kb on + strand, within Pf6N2E2_5397 at 6203.189 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.342 kb on - strand, within Pf6N2E2_5397 at 6203.502 kb on + strand at 6203.504 kb on + strand at 6203.504 kb on + strand at 6203.504 kb on + strand at 6203.504 kb on + strand at 6203.505 kb on - strand at 6203.505 kb on - strand at 6203.505 kb on - strand at 6203.535 kb on - strand at 6203.535 kb on - strand at 6203.535 kb on - strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.566 kb on + strand at 6203.993 kb on - strand, within Pf6N2E2_5399 at 6203.993 kb on - strand, within Pf6N2E2_5399 at 6204.186 kb on + strand, within Pf6N2E2_5399 at 6204.187 kb on - strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.225 kb on + strand, within Pf6N2E2_5399 at 6204.226 kb on - strand, within Pf6N2E2_5399 at 6204.226 kb on - strand, within Pf6N2E2_5399 at 6204.226 kb on - strand, within Pf6N2E2_5399 at 6204.438 kb on + strand, within Pf6N2E2_5399 at 6204.438 kb on + strand, within Pf6N2E2_5399 at 6204.438 kb on + strand, within Pf6N2E2_5399 at 6204.439 kb on - strand, within Pf6N2E2_5399 at 6204.439 kb on - strand, within Pf6N2E2_5399 at 6204.439 kb on - strand, within Pf6N2E2_5399 at 6204.459 kb on + strand, within Pf6N2E2_5399 at 6204.460 kb on - strand, within Pf6N2E2_5399 at 6204.460 kb on - strand, within Pf6N2E2_5399
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 6,201,527 + Pf6N2E2_5395 0.80 +0.1 6,201,528 - Pf6N2E2_5395 0.80 -2.1 6,201,626 + -0.3 6,201,626 + -0.8 6,201,626 + -0.2 6,201,626 + +0.3 6,201,647 + +0.4 6,201,647 + -0.0 6,201,647 + -0.0 6,201,647 + -0.4 6,201,665 + -0.1 6,201,665 + -0.2 6,201,666 - -1.4 6,201,677 + +0.1 6,201,677 + -0.6 6,201,678 - +1.2 6,201,678 - -0.4 6,201,728 + -0.6 6,201,728 + -0.8 6,201,728 + +0.8 6,201,728 + -0.0 6,201,728 + +1.3 6,201,729 - +0.3 6,201,729 - -1.2 6,201,729 - +0.0 6,201,729 - -0.5 6,201,729 - -0.5 6,201,729 - -0.2 6,201,729 - +1.3 6,201,729 - -1.4 6,201,729 - +0.6 6,201,729 - +0.3 6,201,746 + +0.1 6,201,747 - +0.4 6,201,833 + -1.0 6,201,833 + +1.3 6,201,833 + +1.3 6,201,834 - -0.8 6,201,834 - -0.4 6,201,852 + -1.4 6,201,872 + Pf6N2E2_5396 0.11 +1.3 6,201,872 + Pf6N2E2_5396 0.11 +2.3 6,201,872 + Pf6N2E2_5396 0.11 +0.6 6,201,872 + Pf6N2E2_5396 0.11 +0.4 6,201,872 + Pf6N2E2_5396 0.11 -0.4 6,201,872 + Pf6N2E2_5396 0.11 +0.3 6,201,873 - Pf6N2E2_5396 0.11 -0.5 6,201,873 - Pf6N2E2_5396 0.11 -0.7 6,201,873 - Pf6N2E2_5396 0.11 -0.3 6,201,873 - Pf6N2E2_5396 0.11 -0.2 6,201,873 - Pf6N2E2_5396 0.11 -0.6 6,201,873 - Pf6N2E2_5396 0.11 +0.3 6,201,873 - Pf6N2E2_5396 0.11 +2.3 6,201,873 - Pf6N2E2_5396 0.11 +0.3 6,201,873 - Pf6N2E2_5396 0.11 -0.2 6,201,873 - Pf6N2E2_5396 0.11 +1.3 6,201,873 - Pf6N2E2_5396 0.11 +0.3 6,201,873 - Pf6N2E2_5396 0.11 -0.7 6,202,110 + Pf6N2E2_5396 0.49 -0.3 6,202,111 - Pf6N2E2_5396 0.49 +0.7 6,202,162 + Pf6N2E2_5396 0.57 -0.2 6,202,163 - Pf6N2E2_5396 0.57 +0.4 6,202,224 + Pf6N2E2_5396 0.67 +0.3 6,202,224 + Pf6N2E2_5396 0.67 +1.2 6,202,224 + Pf6N2E2_5396 0.67 -0.6 6,202,224 + Pf6N2E2_5396 0.67 +0.3 6,202,224 + Pf6N2E2_5396 0.67 +1.3 6,202,224 + Pf6N2E2_5396 0.67 -0.5 6,202,224 + Pf6N2E2_5396 0.67 -0.1 6,202,224 + Pf6N2E2_5396 0.67 -0.5 6,202,224 + Pf6N2E2_5396 0.67 +0.2 6,202,225 - Pf6N2E2_5396 0.67 -0.7 6,202,225 - Pf6N2E2_5396 0.67 +0.3 6,202,225 - Pf6N2E2_5396 0.67 +0.3 6,202,225 - Pf6N2E2_5396 0.67 -1.0 6,202,225 - Pf6N2E2_5396 0.67 +0.8 6,202,225 - Pf6N2E2_5396 0.67 -0.3 6,202,225 - Pf6N2E2_5396 0.67 -3.0 6,202,297 - Pf6N2E2_5396 0.78 -0.0 6,202,307 + Pf6N2E2_5396 0.80 +0.6 6,202,307 + Pf6N2E2_5396 0.80 +1.3 6,202,308 - Pf6N2E2_5396 0.80 -0.3 6,202,308 - Pf6N2E2_5396 0.80 +0.8 6,202,308 - Pf6N2E2_5396 0.80 +0.6 6,202,308 - Pf6N2E2_5396 0.80 -1.2 6,202,358 + Pf6N2E2_5396 0.88 +0.6 6,202,358 + Pf6N2E2_5396 0.88 -0.3 6,202,722 + Pf6N2E2_5397 0.21 -0.2 6,202,723 - Pf6N2E2_5397 0.21 +0.1 6,202,795 + Pf6N2E2_5397 0.29 -0.4 6,202,796 - Pf6N2E2_5397 0.29 +1.3 6,202,796 - Pf6N2E2_5397 0.29 -1.9 6,202,796 - Pf6N2E2_5397 0.29 +0.2 6,202,796 - Pf6N2E2_5397 0.29 +1.3 6,202,830 + Pf6N2E2_5397 0.32 -0.7 6,202,830 + Pf6N2E2_5397 0.32 -0.2 6,202,830 + Pf6N2E2_5397 0.32 -0.1 6,202,831 - Pf6N2E2_5397 0.32 -0.6 6,202,831 - Pf6N2E2_5397 0.32 -0.3 6,202,831 - Pf6N2E2_5397 0.32 -0.9 6,202,831 - Pf6N2E2_5397 0.32 -0.0 6,202,898 + Pf6N2E2_5397 0.39 -0.5 6,202,898 + Pf6N2E2_5397 0.39 +0.9 6,202,898 + Pf6N2E2_5397 0.39 -0.1 6,202,899 - Pf6N2E2_5397 0.39 +0.3 6,202,899 - Pf6N2E2_5397 0.39 +0.0 6,202,899 - Pf6N2E2_5397 0.39 +0.2 6,202,899 - Pf6N2E2_5397 0.39 -0.4 6,202,899 - Pf6N2E2_5397 0.39 -0.6 6,202,899 - Pf6N2E2_5397 0.39 -0.2 6,202,900 + Pf6N2E2_5397 0.40 +0.4 6,202,900 + Pf6N2E2_5397 0.40 -1.0 6,202,900 + Pf6N2E2_5397 0.40 +1.3 6,202,900 + Pf6N2E2_5397 0.40 +0.1 6,202,900 + Pf6N2E2_5397 0.40 +0.0 6,202,900 + Pf6N2E2_5397 0.40 +0.1 6,202,900 + Pf6N2E2_5397 0.40 +0.5 6,202,900 + Pf6N2E2_5397 0.40 -0.0 6,202,900 + Pf6N2E2_5397 0.40 -0.6 6,202,900 + Pf6N2E2_5397 0.40 -0.7 6,202,901 - Pf6N2E2_5397 0.40 -1.1 6,202,901 - Pf6N2E2_5397 0.40 -0.2 6,202,901 - Pf6N2E2_5397 0.40 -0.6 6,202,901 - Pf6N2E2_5397 0.40 +0.3 6,202,901 - Pf6N2E2_5397 0.40 +0.1 6,202,901 - Pf6N2E2_5397 0.40 +1.1 6,202,901 - Pf6N2E2_5397 0.40 +0.1 6,202,901 - Pf6N2E2_5397 0.40 +0.3 6,202,901 - Pf6N2E2_5397 0.40 +0.1 6,203,178 - Pf6N2E2_5397 0.69 -0.1 6,203,178 - Pf6N2E2_5397 0.69 -0.3 6,203,188 + Pf6N2E2_5397 0.70 -0.3 6,203,188 + Pf6N2E2_5397 0.70 +0.4 6,203,189 - Pf6N2E2_5397 0.70 +0.5 6,203,237 - Pf6N2E2_5397 0.75 -0.1 6,203,237 - Pf6N2E2_5397 0.75 +0.9 6,203,237 - Pf6N2E2_5397 0.75 -0.1 6,203,237 - Pf6N2E2_5397 0.75 +1.3 6,203,237 - Pf6N2E2_5397 0.75 +0.3 6,203,237 - Pf6N2E2_5397 0.75 -0.5 6,203,237 - Pf6N2E2_5397 0.75 +0.1 6,203,342 - Pf6N2E2_5397 0.86 -1.3 6,203,502 + -2.2 6,203,504 + -0.6 6,203,504 + +1.5 6,203,504 + +0.1 6,203,504 + -1.3 6,203,505 - -1.1 6,203,505 - -0.4 6,203,505 - +0.0 6,203,535 - -0.0 6,203,535 - -0.1 6,203,535 - +0.2 6,203,566 + -0.4 6,203,566 + -0.3 6,203,566 + +0.4 6,203,566 + -0.7 6,203,566 + +0.3 6,203,566 + -0.2 6,203,566 + +0.2 6,203,566 + +0.1 6,203,566 + +0.5 6,203,566 + +0.1 6,203,993 - Pf6N2E2_5399 0.13 +1.3 6,203,993 - Pf6N2E2_5399 0.13 +0.3 6,204,186 + Pf6N2E2_5399 0.38 +0.4 6,204,187 - Pf6N2E2_5399 0.38 +0.2 6,204,225 + Pf6N2E2_5399 0.43 +0.3 6,204,225 + Pf6N2E2_5399 0.43 +0.0 6,204,225 + Pf6N2E2_5399 0.43 -0.5 6,204,225 + Pf6N2E2_5399 0.43 -0.2 6,204,225 + Pf6N2E2_5399 0.43 +0.0 6,204,225 + Pf6N2E2_5399 0.43 -0.9 6,204,225 + Pf6N2E2_5399 0.43 -0.0 6,204,225 + Pf6N2E2_5399 0.43 -0.2 6,204,226 - Pf6N2E2_5399 0.43 -1.2 6,204,226 - Pf6N2E2_5399 0.43 +0.1 6,204,226 - Pf6N2E2_5399 0.43 +0.1 6,204,438 + Pf6N2E2_5399 0.71 -0.1 6,204,438 + Pf6N2E2_5399 0.71 +0.1 6,204,438 + Pf6N2E2_5399 0.71 +0.9 6,204,439 - Pf6N2E2_5399 0.71 -0.1 6,204,439 - Pf6N2E2_5399 0.71 +0.4 6,204,439 - Pf6N2E2_5399 0.71 +0.1 6,204,459 + Pf6N2E2_5399 0.74 +0.4 6,204,460 - Pf6N2E2_5399 0.74 -2.6 6,204,460 - Pf6N2E2_5399 0.74 +0.8
Or see this region's nucleotide sequence