Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5223

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
6,020,999	+				+0.3
6,021,013	+				-1.2
6,021,013	+				-0.3
6,021,013	+				+0.2
6,021,978	+		Pf6N2E2_5223	0.18	-4.3
6,021,978	+		Pf6N2E2_5223	0.18	-2.2
6,021,978	+		Pf6N2E2_5223	0.18	-2.3
6,021,978	+		Pf6N2E2_5223	0.18	-2.3
6,022,271	+		Pf6N2E2_5223	0.57	-3.7
6,022,353	+		Pf6N2E2_5223	0.68	-1.9
6,022,365	+		Pf6N2E2_5223	0.69	-4.3
6,022,365	+		Pf6N2E2_5223	0.69	-3.7
6,023,334	+				-2.8
6,023,334	+				-0.8
6,023,334	+				-2.4
6,023,339	+				-0.1
6,023,436	-				-0.3
6,023,437	+				-0.6
6,023,466	+				-1.2
6,023,466	+				-0.1
6,023,466	+				+0.1
6,023,466	+				+0.2
6,023,466	+				+1.1
6,023,467	-				+0.2
6,023,467	-				+0.3
6,023,548	+		Pf6N2E2_5225	0.10	+0.5
6,023,548	+		Pf6N2E2_5225	0.10	-0.1
6,023,548	+		Pf6N2E2_5225	0.10	-0.8
6,023,548	+		Pf6N2E2_5225	0.10	+0.3
6,023,548	+		Pf6N2E2_5225	0.10	-0.2
6,023,548	+		Pf6N2E2_5225	0.10	-0.5
6,023,548	+		Pf6N2E2_5225	0.10	+1.5
6,023,548	+		Pf6N2E2_5225	0.10	+0.2
6,023,548	+		Pf6N2E2_5225	0.10	+0.2
6,023,549	-		Pf6N2E2_5225	0.11	-0.0
6,023,549	-		Pf6N2E2_5225	0.11	+0.2
6,023,549	-		Pf6N2E2_5225	0.11	+0.5