Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_468 and Pf6N2E2_469 are separated by 251 nucleotides Pf6N2E2_469 and Pf6N2E2_470 overlap by 4 nucleotides Pf6N2E2_470 and Pf6N2E2_471 overlap by 1 nucleotides Pf6N2E2_471 and Pf6N2E2_472 are separated by 288 nucleotides Pf6N2E2_472 and Pf6N2E2_473 are separated by 13 nucleotides
Pf6N2E2_468 - DNA alkylation repair enzyme, at 534,448 to 535,551
_468
Pf6N2E2_469 - lipoprotein, putative, at 535,803 to 536,111
_469
Pf6N2E2_470 - hypothetical protein, at 536,108 to 536,431
_470
Pf6N2E2_471 - putative metalloprotease, at 536,431 to 537,492
_471
Pf6N2E2_472 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 537,781 to 538,122
_472
Pf6N2E2_473 - putative cytochrome p450 oxidoreductase, at 538,136 to 539,272
_473
Position (kb) 536
537
538 Strain fitness (log2 ratio) -1
0
1
2 at 535.460 kb on + strand at 535.581 kb on + strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.584 kb on - strand at 535.584 kb on - strand at 535.740 kb on - strand at 536.052 kb on + strand, within Pf6N2E2_469 at 536.052 kb on + strand, within Pf6N2E2_469 at 536.053 kb on - strand, within Pf6N2E2_469 at 536.110 kb on - strand at 536.110 kb on - strand at 536.591 kb on + strand at 536.648 kb on + strand, within Pf6N2E2_471 at 536.648 kb on + strand, within Pf6N2E2_471 at 536.648 kb on + strand, within Pf6N2E2_471 at 536.649 kb on - strand, within Pf6N2E2_471 at 536.649 kb on - strand, within Pf6N2E2_471 at 536.873 kb on - strand, within Pf6N2E2_471 at 536.942 kb on + strand, within Pf6N2E2_471 at 536.943 kb on - strand, within Pf6N2E2_471 at 537.095 kb on + strand, within Pf6N2E2_471 at 537.096 kb on - strand, within Pf6N2E2_471 at 537.127 kb on - strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.171 kb on - strand, within Pf6N2E2_471 at 537.171 kb on - strand, within Pf6N2E2_471 at 537.452 kb on + strand at 537.452 kb on + strand at 537.452 kb on + strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.505 kb on - strand at 537.833 kb on + strand, within Pf6N2E2_472 at 537.833 kb on + strand, within Pf6N2E2_472 at 537.833 kb on + strand, within Pf6N2E2_472 at 537.904 kb on + strand, within Pf6N2E2_472 at 537.904 kb on + strand, within Pf6N2E2_472 at 537.905 kb on - strand, within Pf6N2E2_472 at 537.905 kb on - strand, within Pf6N2E2_472 at 538.031 kb on - strand, within Pf6N2E2_472 at 538.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 535,460 + +1.8 535,581 + +0.1 535,582 - -0.6 535,582 - +0.1 535,582 - +0.1 535,582 - +1.4 535,584 - +1.4 535,584 - +0.7 535,740 - -0.1 536,052 + Pf6N2E2_469 0.81 -0.2 536,052 + Pf6N2E2_469 0.81 +1.4 536,053 - Pf6N2E2_469 0.81 -0.5 536,110 - +0.2 536,110 - +1.4 536,591 + +1.4 536,648 + Pf6N2E2_471 0.20 +0.7 536,648 + Pf6N2E2_471 0.20 +0.9 536,648 + Pf6N2E2_471 0.20 +0.1 536,649 - Pf6N2E2_471 0.21 +0.7 536,649 - Pf6N2E2_471 0.21 +0.8 536,873 - Pf6N2E2_471 0.42 +0.1 536,942 + Pf6N2E2_471 0.48 +1.4 536,943 - Pf6N2E2_471 0.48 +0.6 537,095 + Pf6N2E2_471 0.63 +0.6 537,096 - Pf6N2E2_471 0.63 -0.9 537,127 - Pf6N2E2_471 0.66 -0.0 537,170 + Pf6N2E2_471 0.70 -0.1 537,170 + Pf6N2E2_471 0.70 +0.5 537,170 + Pf6N2E2_471 0.70 +0.3 537,170 + Pf6N2E2_471 0.70 -0.4 537,171 - Pf6N2E2_471 0.70 +0.5 537,171 - Pf6N2E2_471 0.70 +0.7 537,452 + -1.5 537,452 + +0.3 537,452 + +0.6 537,453 - +0.9 537,453 - +1.4 537,453 - +2.4 537,453 - -1.5 537,505 - -0.6 537,833 + Pf6N2E2_472 0.15 -0.3 537,833 + Pf6N2E2_472 0.15 +0.5 537,833 + Pf6N2E2_472 0.15 -1.3 537,904 + Pf6N2E2_472 0.36 +0.2 537,904 + Pf6N2E2_472 0.36 -0.7 537,905 - Pf6N2E2_472 0.36 +1.4 537,905 - Pf6N2E2_472 0.36 +1.4 538,031 - Pf6N2E2_472 0.73 +0.7 538,212 - -0.3
Or see this region's nucleotide sequence