Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4611 and Pf6N2E2_4612 are separated by 77 nucleotides Pf6N2E2_4612 and Pf6N2E2_4613 are separated by 152 nucleotides Pf6N2E2_4613 and Pf6N2E2_4614 are separated by 48 nucleotides
Pf6N2E2_4611 - Ferric siderophore transport system, periplasmic binding protein TonB, at 5,335,924 to 5,336,820
_4611
Pf6N2E2_4612 - Glutathione synthetase (EC 6.3.2.3), at 5,336,898 to 5,337,851
_4612
Pf6N2E2_4613 - twitching motility protein PilG, at 5,338,004 to 5,338,504
_4613
Pf6N2E2_4614 - twitching motility protein PilH, at 5,338,553 to 5,338,918
_4614
Position (kb) 5336
5337
5338 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5335.911 kb on + strand at 5335.911 kb on + strand at 5335.912 kb on - strand at 5335.912 kb on - strand at 5335.912 kb on - strand at 5336.083 kb on + strand, within Pf6N2E2_4611 at 5336.083 kb on + strand, within Pf6N2E2_4611 at 5336.099 kb on + strand, within Pf6N2E2_4611 at 5336.099 kb on + strand, within Pf6N2E2_4611 at 5336.100 kb on - strand, within Pf6N2E2_4611 at 5336.148 kb on - strand, within Pf6N2E2_4611 at 5336.291 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.863 kb on + strand at 5337.188 kb on - strand, within Pf6N2E2_4612 at 5337.188 kb on - strand, within Pf6N2E2_4612 at 5337.250 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.792 kb on - strand at 5337.800 kb on + strand at 5337.803 kb on - strand at 5337.843 kb on + strand at 5337.844 kb on - strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.984 kb on + strand at 5338.047 kb on + strand at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.596 kb on - strand, within Pf6N2E2_4614
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 5,335,911 + +0.1 5,335,911 + -0.3 5,335,912 - -0.2 5,335,912 - +0.4 5,335,912 - +0.1 5,336,083 + Pf6N2E2_4611 0.18 +1.1 5,336,083 + Pf6N2E2_4611 0.18 +0.1 5,336,099 + Pf6N2E2_4611 0.20 +1.1 5,336,099 + Pf6N2E2_4611 0.20 +1.1 5,336,100 - Pf6N2E2_4611 0.20 +1.1 5,336,148 - Pf6N2E2_4611 0.25 -0.9 5,336,291 + Pf6N2E2_4611 0.41 +1.1 5,336,517 + Pf6N2E2_4611 0.66 +1.1 5,336,517 + Pf6N2E2_4611 0.66 +1.1 5,336,517 + Pf6N2E2_4611 0.66 +1.1 5,336,517 + Pf6N2E2_4611 0.66 +0.1 5,336,517 + Pf6N2E2_4611 0.66 +1.1 5,336,517 + Pf6N2E2_4611 0.66 +1.1 5,336,863 + -0.2 5,337,188 - Pf6N2E2_4612 0.30 +1.1 5,337,188 - Pf6N2E2_4612 0.30 +0.6 5,337,250 + Pf6N2E2_4612 0.37 +1.1 5,337,550 + Pf6N2E2_4612 0.68 -2.7 5,337,550 + Pf6N2E2_4612 0.68 -1.2 5,337,550 + Pf6N2E2_4612 0.68 +1.1 5,337,550 + Pf6N2E2_4612 0.68 +1.1 5,337,550 + Pf6N2E2_4612 0.68 -1.7 5,337,551 - Pf6N2E2_4612 0.68 -2.4 5,337,551 - Pf6N2E2_4612 0.68 -2.9 5,337,551 - Pf6N2E2_4612 0.68 -2.3 5,337,559 + Pf6N2E2_4612 0.69 -2.4 5,337,559 + Pf6N2E2_4612 0.69 +2.1 5,337,740 - Pf6N2E2_4612 0.88 -1.7 5,337,740 - Pf6N2E2_4612 0.88 -1.4 5,337,792 - +0.1 5,337,800 + -1.4 5,337,803 - -2.3 5,337,843 + -1.4 5,337,844 - -2.3 5,337,882 + -1.0 5,337,882 + -0.1 5,337,882 + -1.6 5,337,882 + -1.2 5,337,882 + -0.8 5,337,883 - +0.2 5,337,883 - -0.1 5,337,883 - +1.1 5,337,883 - +0.5 5,337,883 - -0.5 5,337,883 - -1.1 5,337,883 - +0.1 5,337,883 - -0.6 5,337,883 - -0.7 5,337,984 + +0.2 5,338,047 + +1.9 5,338,595 + Pf6N2E2_4614 0.11 -0.3 5,338,595 + Pf6N2E2_4614 0.11 +0.6 5,338,595 + Pf6N2E2_4614 0.11 +1.1 5,338,596 - Pf6N2E2_4614 0.12 +0.3
Or see this region's nucleotide sequence