Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_263 and Pf6N2E2_264 are separated by 231 nucleotides Pf6N2E2_264 and Pf6N2E2_265 are separated by 145 nucleotides
Pf6N2E2_263 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 326,556 to 327,200
_263
Pf6N2E2_264 - Transcriptional regulator, TetR family, at 327,432 to 327,887
_264
Pf6N2E2_265 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 328,033 to 329,496
_265
Position (kb) 327
328 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 326.465 kb on + strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.553 kb on + strand at 326.553 kb on + strand at 326.554 kb on - strand at 326.554 kb on - strand at 326.554 kb on - strand at 326.558 kb on - strand at 326.558 kb on - strand at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.737 kb on + strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.784 kb on + strand, within Pf6N2E2_263 at 326.849 kb on + strand, within Pf6N2E2_263 at 326.850 kb on - strand, within Pf6N2E2_263 at 326.980 kb on - strand, within Pf6N2E2_263 at 327.129 kb on + strand, within Pf6N2E2_263 at 327.129 kb on + strand, within Pf6N2E2_263 at 327.130 kb on - strand, within Pf6N2E2_263 at 327.151 kb on - strand at 327.151 kb on - strand at 327.266 kb on + strand at 327.286 kb on + strand at 327.298 kb on - strand at 327.298 kb on - strand at 327.305 kb on + strand at 327.305 kb on + strand at 327.305 kb on + strand at 327.305 kb on + strand at 327.305 kb on + strand at 327.306 kb on - strand at 327.306 kb on - strand at 327.306 kb on - strand at 327.309 kb on + strand at 327.309 kb on + strand at 327.309 kb on + strand at 327.309 kb on + strand at 327.310 kb on - strand at 327.386 kb on - strand at 327.386 kb on - strand at 327.450 kb on + strand at 327.450 kb on + strand at 327.451 kb on - strand at 327.513 kb on + strand, within Pf6N2E2_264 at 327.576 kb on + strand, within Pf6N2E2_264 at 327.577 kb on - strand, within Pf6N2E2_264 at 327.584 kb on + strand, within Pf6N2E2_264 at 327.584 kb on + strand, within Pf6N2E2_264 at 327.748 kb on + strand, within Pf6N2E2_264 at 327.890 kb on + strand at 327.891 kb on - strand at 327.911 kb on + strand at 328.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 326,465 + -1.1 326,465 + -0.6 326,465 + -1.3 326,465 + -0.6 326,466 - -1.0 326,466 - -1.4 326,466 - +0.2 326,466 - -0.4 326,553 + +0.4 326,553 + +0.6 326,554 - +0.4 326,554 - -1.2 326,554 - -0.2 326,558 - +0.4 326,558 - -1.3 326,644 + Pf6N2E2_263 0.14 -0.6 326,644 + Pf6N2E2_263 0.14 -0.2 326,644 + Pf6N2E2_263 0.14 -0.7 326,644 + Pf6N2E2_263 0.14 -0.7 326,645 - Pf6N2E2_263 0.14 -0.4 326,645 - Pf6N2E2_263 0.14 -0.2 326,645 - Pf6N2E2_263 0.14 +0.3 326,645 - Pf6N2E2_263 0.14 +0.9 326,737 + Pf6N2E2_263 0.28 -1.0 326,738 - Pf6N2E2_263 0.28 -0.7 326,738 - Pf6N2E2_263 0.28 +2.4 326,738 - Pf6N2E2_263 0.28 -1.0 326,738 - Pf6N2E2_263 0.28 +1.9 326,784 + Pf6N2E2_263 0.35 -1.5 326,849 + Pf6N2E2_263 0.45 -1.1 326,850 - Pf6N2E2_263 0.46 -0.2 326,980 - Pf6N2E2_263 0.66 -0.6 327,129 + Pf6N2E2_263 0.89 +0.1 327,129 + Pf6N2E2_263 0.89 -0.7 327,130 - Pf6N2E2_263 0.89 -0.2 327,151 - +0.7 327,151 - -0.3 327,266 + -0.8 327,286 + -1.0 327,298 - +0.2 327,298 - -0.7 327,305 + -0.2 327,305 + -1.5 327,305 + -1.4 327,305 + -1.0 327,305 + -0.5 327,306 - -0.2 327,306 - -0.5 327,306 - +0.5 327,309 + -1.3 327,309 + -0.2 327,309 + +0.4 327,309 + -1.8 327,310 - +0.1 327,386 - +1.4 327,386 - -0.1 327,450 + -0.3 327,450 + +1.4 327,451 - -0.2 327,513 + Pf6N2E2_264 0.18 -0.2 327,576 + Pf6N2E2_264 0.32 -1.4 327,577 - Pf6N2E2_264 0.32 -0.9 327,584 + Pf6N2E2_264 0.33 -1.1 327,584 + Pf6N2E2_264 0.33 -1.3 327,748 + Pf6N2E2_264 0.69 -1.9 327,890 + -1.6 327,891 - +2.9 327,911 + -2.1 328,014 - -1.0
Or see this region's nucleotide sequence