Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2303 and Pf6N2E2_2304 are separated by 91 nucleotides Pf6N2E2_2304 and Pf6N2E2_2305 are separated by 96 nucleotides Pf6N2E2_2305 and Pf6N2E2_2306 are separated by 10 nucleotides Pf6N2E2_2306 and Pf6N2E2_2307 are separated by 27 nucleotides Pf6N2E2_2307 and Pf6N2E2_2308 overlap by 4 nucleotides
Pf6N2E2_2303 - Aerotaxis receptor Aer, at 2,728,083 to 2,729,648
_2303
Pf6N2E2_2304 - Predicted hydrolase of the alpha/beta-hydrolase fold, at 2,729,740 to 2,730,432
_2304
Pf6N2E2_2305 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 2,730,529 to 2,731,953
_2305
Pf6N2E2_2306 - Cytochrome c oxidase subunit CcoO (EC 1.9.3.1), at 2,731,964 to 2,732,572
_2306
Pf6N2E2_2307 - Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1), at 2,732,600 to 2,732,779
_2307
Pf6N2E2_2308 - Cytochrome c oxidase subunit CcoP (EC 1.9.3.1), at 2,732,776 to 2,733,732
_2308
Position (kb)
2730
2731
2732 Strain fitness (log2 ratio)
-1
0
1
2 at 2729.531 kb on + strand at 2729.531 kb on + strand at 2729.532 kb on - strand at 2729.532 kb on - strand at 2729.612 kb on + strand at 2729.612 kb on + strand at 2729.640 kb on - strand at 2729.787 kb on + strand at 2729.787 kb on + strand at 2729.788 kb on - strand at 2729.788 kb on - strand at 2729.788 kb on - strand at 2729.788 kb on - strand at 2729.999 kb on + strand, within Pf6N2E2_2304 at 2729.999 kb on + strand, within Pf6N2E2_2304 at 2729.999 kb on + strand, within Pf6N2E2_2304 at 2730.000 kb on - strand, within Pf6N2E2_2304 at 2730.000 kb on - strand, within Pf6N2E2_2304 at 2730.000 kb on - strand, within Pf6N2E2_2304 at 2730.000 kb on - strand, within Pf6N2E2_2304 at 2730.000 kb on - strand, within Pf6N2E2_2304 at 2730.000 kb on - strand, within Pf6N2E2_2304 at 2730.064 kb on - strand, within Pf6N2E2_2304 at 2730.064 kb on - strand, within Pf6N2E2_2304 at 2730.111 kb on - strand, within Pf6N2E2_2304 at 2730.128 kb on + strand, within Pf6N2E2_2304 at 2730.129 kb on - strand, within Pf6N2E2_2304 at 2730.210 kb on - strand, within Pf6N2E2_2304 at 2730.210 kb on - strand, within Pf6N2E2_2304 at 2730.210 kb on - strand, within Pf6N2E2_2304 at 2730.456 kb on + strand at 2730.456 kb on + strand at 2730.540 kb on + strand at 2730.541 kb on - strand at 2730.554 kb on - strand at 2730.559 kb on + strand at 2730.559 kb on + strand at 2730.559 kb on + strand at 2730.560 kb on - strand at 2730.560 kb on - strand at 2730.560 kb on - strand at 2730.585 kb on + strand at 2730.586 kb on - strand at 2730.586 kb on - strand at 2730.763 kb on + strand, within Pf6N2E2_2305 at 2730.764 kb on - strand, within Pf6N2E2_2305 at 2730.764 kb on - strand, within Pf6N2E2_2305 at 2730.764 kb on - strand, within Pf6N2E2_2305 at 2730.877 kb on + strand, within Pf6N2E2_2305 at 2730.895 kb on + strand, within Pf6N2E2_2305 at 2731.000 kb on + strand, within Pf6N2E2_2305 at 2731.000 kb on + strand, within Pf6N2E2_2305 at 2731.000 kb on + strand, within Pf6N2E2_2305 at 2731.001 kb on - strand, within Pf6N2E2_2305 at 2731.001 kb on - strand, within Pf6N2E2_2305 at 2731.001 kb on - strand, within Pf6N2E2_2305 at 2731.001 kb on - strand, within Pf6N2E2_2305 at 2731.001 kb on - strand, within Pf6N2E2_2305 at 2731.001 kb on - strand, within Pf6N2E2_2305 at 2731.001 kb on - strand, within Pf6N2E2_2305 at 2731.001 kb on - strand, within Pf6N2E2_2305 at 2731.009 kb on - strand, within Pf6N2E2_2305 at 2731.009 kb on - strand, within Pf6N2E2_2305 at 2731.096 kb on + strand, within Pf6N2E2_2305 at 2731.142 kb on - strand, within Pf6N2E2_2305 at 2731.195 kb on + strand, within Pf6N2E2_2305 at 2731.196 kb on - strand, within Pf6N2E2_2305 at 2731.196 kb on - strand at 2731.196 kb on - strand, within Pf6N2E2_2305 at 2731.198 kb on + strand, within Pf6N2E2_2305 at 2731.199 kb on - strand, within Pf6N2E2_2305 at 2731.199 kb on - strand, within Pf6N2E2_2305 at 2731.231 kb on + strand, within Pf6N2E2_2305 at 2731.232 kb on - strand, within Pf6N2E2_2305 at 2731.279 kb on + strand, within Pf6N2E2_2305 at 2731.279 kb on + strand, within Pf6N2E2_2305 at 2731.280 kb on - strand, within Pf6N2E2_2305 at 2731.280 kb on - strand, within Pf6N2E2_2305 at 2731.280 kb on - strand, within Pf6N2E2_2305 at 2731.310 kb on - strand, within Pf6N2E2_2305 at 2731.478 kb on - strand at 2731.540 kb on + strand, within Pf6N2E2_2305 at 2731.565 kb on + strand, within Pf6N2E2_2305 at 2731.615 kb on + strand, within Pf6N2E2_2305 at 2731.616 kb on - strand, within Pf6N2E2_2305 at 2731.712 kb on - strand, within Pf6N2E2_2305 at 2731.789 kb on + strand, within Pf6N2E2_2305 at 2731.790 kb on - strand, within Pf6N2E2_2305 at 2731.790 kb on - strand, within Pf6N2E2_2305 at 2731.885 kb on + strand at 2731.885 kb on + strand at 2731.885 kb on + strand at 2731.886 kb on - strand at 2732.138 kb on + strand, within Pf6N2E2_2306 at 2732.138 kb on + strand, within Pf6N2E2_2306 at 2732.138 kb on + strand, within Pf6N2E2_2306 at 2732.138 kb on + strand, within Pf6N2E2_2306 at 2732.139 kb on - strand, within Pf6N2E2_2306 at 2732.336 kb on + strand, within Pf6N2E2_2306 at 2732.337 kb on - strand, within Pf6N2E2_2306 at 2732.594 kb on - strand at 2732.594 kb on - strand at 2732.662 kb on - strand, within Pf6N2E2_2307 at 2732.793 kb on + strand at 2732.793 kb on + strand at 2732.793 kb on + strand at 2732.794 kb on - strand at 2732.924 kb on - strand, within Pf6N2E2_2308 at 2732.924 kb on - strand, within Pf6N2E2_2308
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 2,729,531 + -0.6 2,729,531 + +0.1 2,729,532 - +1.4 2,729,532 - -0.5 2,729,612 + -0.1 2,729,612 + +1.4 2,729,640 - +1.2 2,729,787 + +1.4 2,729,787 + -1.5 2,729,788 - +0.2 2,729,788 - -0.1 2,729,788 - -0.4 2,729,788 - -0.6 2,729,999 + Pf6N2E2_2304 0.37 +1.4 2,729,999 + Pf6N2E2_2304 0.37 -0.8 2,729,999 + Pf6N2E2_2304 0.37 +0.5 2,730,000 - Pf6N2E2_2304 0.38 -0.9 2,730,000 - Pf6N2E2_2304 0.38 +0.6 2,730,000 - Pf6N2E2_2304 0.38 -0.6 2,730,000 - Pf6N2E2_2304 0.38 +0.8 2,730,000 - Pf6N2E2_2304 0.38 -1.1 2,730,000 - Pf6N2E2_2304 0.38 -0.7 2,730,064 - Pf6N2E2_2304 0.47 -0.0 2,730,064 - Pf6N2E2_2304 0.47 +2.4 2,730,111 - Pf6N2E2_2304 0.54 +2.1 2,730,128 + Pf6N2E2_2304 0.56 +1.4 2,730,129 - Pf6N2E2_2304 0.56 +0.9 2,730,210 - Pf6N2E2_2304 0.68 +0.2 2,730,210 - Pf6N2E2_2304 0.68 +0.4 2,730,210 - Pf6N2E2_2304 0.68 -0.2 2,730,456 + +1.0 2,730,456 + +1.1 2,730,540 + +0.7 2,730,541 - +1.1 2,730,554 - +0.1 2,730,559 + -0.2 2,730,559 + +0.6 2,730,559 + +0.9 2,730,560 - +1.4 2,730,560 - +0.5 2,730,560 - +0.5 2,730,585 + +0.4 2,730,586 - +0.0 2,730,586 - +0.9 2,730,763 + Pf6N2E2_2305 0.16 +1.3 2,730,764 - Pf6N2E2_2305 0.16 +0.7 2,730,764 - Pf6N2E2_2305 0.16 +1.6 2,730,764 - Pf6N2E2_2305 0.16 +0.1 2,730,877 + Pf6N2E2_2305 0.24 +0.8 2,730,895 + Pf6N2E2_2305 0.26 +1.4 2,731,000 + Pf6N2E2_2305 0.33 +0.6 2,731,000 + Pf6N2E2_2305 0.33 +1.8 2,731,000 + Pf6N2E2_2305 0.33 +1.0 2,731,001 - Pf6N2E2_2305 0.33 +0.7 2,731,001 - Pf6N2E2_2305 0.33 -0.1 2,731,001 - Pf6N2E2_2305 0.33 -0.2 2,731,001 - Pf6N2E2_2305 0.33 +0.9 2,731,001 - Pf6N2E2_2305 0.33 +2.4 2,731,001 - Pf6N2E2_2305 0.33 +0.5 2,731,001 - Pf6N2E2_2305 0.33 -0.3 2,731,001 - Pf6N2E2_2305 0.33 -0.7 2,731,009 - Pf6N2E2_2305 0.34 +1.4 2,731,009 - Pf6N2E2_2305 0.34 +1.4 2,731,096 + Pf6N2E2_2305 0.40 -0.5 2,731,142 - Pf6N2E2_2305 0.43 +1.3 2,731,195 + Pf6N2E2_2305 0.47 +1.2 2,731,196 - Pf6N2E2_2305 0.47 +1.1 2,731,196 - +0.4 2,731,196 - Pf6N2E2_2305 0.47 +0.9 2,731,198 + Pf6N2E2_2305 0.47 +0.1 2,731,199 - Pf6N2E2_2305 0.47 +1.3 2,731,199 - Pf6N2E2_2305 0.47 +0.9 2,731,231 + Pf6N2E2_2305 0.49 -0.2 2,731,232 - Pf6N2E2_2305 0.49 +0.4 2,731,279 + Pf6N2E2_2305 0.53 +0.0 2,731,279 + Pf6N2E2_2305 0.53 +1.5 2,731,280 - Pf6N2E2_2305 0.53 -0.3 2,731,280 - Pf6N2E2_2305 0.53 +0.3 2,731,280 - Pf6N2E2_2305 0.53 +1.9 2,731,310 - Pf6N2E2_2305 0.55 +0.3 2,731,478 - +0.4 2,731,540 + Pf6N2E2_2305 0.71 +1.1 2,731,565 + Pf6N2E2_2305 0.73 +0.1 2,731,615 + Pf6N2E2_2305 0.76 -0.9 2,731,616 - Pf6N2E2_2305 0.76 +0.2 2,731,712 - Pf6N2E2_2305 0.83 +0.7 2,731,789 + Pf6N2E2_2305 0.88 +2.4 2,731,790 - Pf6N2E2_2305 0.88 +0.6 2,731,790 - Pf6N2E2_2305 0.88 +1.3 2,731,885 + +0.7 2,731,885 + +0.1 2,731,885 + +1.9 2,731,886 - +1.4 2,732,138 + Pf6N2E2_2306 0.29 +0.8 2,732,138 + Pf6N2E2_2306 0.29 +1.8 2,732,138 + Pf6N2E2_2306 0.29 -0.8 2,732,138 + Pf6N2E2_2306 0.29 +1.3 2,732,139 - Pf6N2E2_2306 0.29 -1.2 2,732,336 + Pf6N2E2_2306 0.61 +0.8 2,732,337 - Pf6N2E2_2306 0.61 +0.6 2,732,594 - +0.5 2,732,594 - +1.0 2,732,662 - Pf6N2E2_2307 0.34 +0.6 2,732,793 + +0.8 2,732,793 + +0.4 2,732,793 + +1.5 2,732,794 - -0.6 2,732,924 - Pf6N2E2_2308 0.15 +0.4 2,732,924 - Pf6N2E2_2308 0.15 +1.6
Or see this region's nucleotide sequence