Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_229 and Pf6N2E2_230 are separated by 345 nucleotides Pf6N2E2_230 and Pf6N2E2_231 are separated by 35 nucleotides
Pf6N2E2_229 - Putative fumarylacetoacetate (FAA) hydrolase, at 291,221 to 292,072
_229
Pf6N2E2_230 - lignostilbene-alpha,beta-dioxygenase, at 292,418 to 293,866
_230
Pf6N2E2_231 - FIG00956946: hypothetical protein, at 293,902 to 295,083
_231
Position (kb)
292
293
294 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 291.607 kb on - strand, within Pf6N2E2_229 at 291.607 kb on - strand, within Pf6N2E2_229 at 291.607 kb on - strand, within Pf6N2E2_229 at 291.784 kb on - strand, within Pf6N2E2_229 at 291.826 kb on - strand, within Pf6N2E2_229 at 292.032 kb on + strand at 292.032 kb on + strand at 292.032 kb on + strand at 292.032 kb on + strand at 292.033 kb on - strand at 292.036 kb on + strand at 292.037 kb on - strand at 292.037 kb on - strand at 292.039 kb on + strand at 292.055 kb on - strand at 292.055 kb on - strand at 292.110 kb on - strand at 292.196 kb on - strand at 292.196 kb on - strand at 292.337 kb on + strand at 292.338 kb on - strand at 292.388 kb on + strand at 292.388 kb on + strand at 292.388 kb on + strand at 292.389 kb on - strand at 292.389 kb on - strand at 292.570 kb on - strand, within Pf6N2E2_230 at 292.570 kb on - strand, within Pf6N2E2_230 at 292.570 kb on - strand, within Pf6N2E2_230 at 292.709 kb on + strand, within Pf6N2E2_230 at 292.710 kb on - strand, within Pf6N2E2_230 at 292.731 kb on + strand, within Pf6N2E2_230 at 292.732 kb on - strand, within Pf6N2E2_230 at 292.732 kb on - strand, within Pf6N2E2_230 at 292.789 kb on - strand, within Pf6N2E2_230 at 292.789 kb on - strand, within Pf6N2E2_230 at 292.821 kb on + strand, within Pf6N2E2_230 at 292.822 kb on - strand, within Pf6N2E2_230 at 292.822 kb on - strand, within Pf6N2E2_230 at 292.916 kb on + strand, within Pf6N2E2_230 at 292.916 kb on + strand, within Pf6N2E2_230 at 292.917 kb on - strand, within Pf6N2E2_230 at 292.920 kb on + strand, within Pf6N2E2_230 at 292.921 kb on - strand, within Pf6N2E2_230 at 292.944 kb on + strand, within Pf6N2E2_230 at 292.944 kb on + strand, within Pf6N2E2_230 at 292.945 kb on - strand, within Pf6N2E2_230 at 292.945 kb on - strand, within Pf6N2E2_230 at 292.945 kb on - strand, within Pf6N2E2_230 at 292.945 kb on - strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.980 kb on + strand, within Pf6N2E2_230 at 292.981 kb on - strand, within Pf6N2E2_230 at 292.981 kb on - strand, within Pf6N2E2_230 at 293.014 kb on + strand, within Pf6N2E2_230 at 293.015 kb on - strand, within Pf6N2E2_230 at 293.227 kb on - strand, within Pf6N2E2_230 at 293.284 kb on - strand, within Pf6N2E2_230 at 293.319 kb on + strand, within Pf6N2E2_230 at 293.319 kb on + strand, within Pf6N2E2_230 at 293.547 kb on + strand, within Pf6N2E2_230 at 293.548 kb on - strand, within Pf6N2E2_230 at 293.548 kb on - strand, within Pf6N2E2_230 at 293.820 kb on + strand at 293.989 kb on + strand at 293.996 kb on + strand at 293.997 kb on - strand at 293.997 kb on - strand at 294.151 kb on + strand, within Pf6N2E2_231 at 294.152 kb on - strand, within Pf6N2E2_231 at 294.152 kb on - strand, within Pf6N2E2_231 at 294.155 kb on + strand, within Pf6N2E2_231 at 294.180 kb on + strand, within Pf6N2E2_231 at 294.181 kb on - strand, within Pf6N2E2_231 at 294.227 kb on + strand, within Pf6N2E2_231 at 294.227 kb on + strand, within Pf6N2E2_231 at 294.287 kb on - strand, within Pf6N2E2_231 at 294.413 kb on + strand, within Pf6N2E2_231 at 294.413 kb on + strand, within Pf6N2E2_231 at 294.413 kb on + strand, within Pf6N2E2_231 at 294.414 kb on - strand, within Pf6N2E2_231
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 291,607 - Pf6N2E2_229 0.45 +0.2 291,607 - Pf6N2E2_229 0.45 -0.6 291,607 - Pf6N2E2_229 0.45 -0.8 291,784 - Pf6N2E2_229 0.66 -0.7 291,826 - Pf6N2E2_229 0.71 +0.3 292,032 + +1.4 292,032 + -1.4 292,032 + -0.9 292,032 + +1.4 292,033 - +0.6 292,036 + +0.4 292,037 - -0.0 292,037 - -1.2 292,039 + +1.2 292,055 - -0.3 292,055 - -0.6 292,110 - -0.2 292,196 - +1.4 292,196 - -0.2 292,337 + -0.5 292,338 - +1.4 292,388 + -0.6 292,388 + -0.5 292,388 + -0.1 292,389 - -1.8 292,389 - -0.6 292,570 - Pf6N2E2_230 0.10 -1.0 292,570 - Pf6N2E2_230 0.10 -0.4 292,570 - Pf6N2E2_230 0.10 -0.1 292,709 + Pf6N2E2_230 0.20 +0.0 292,710 - Pf6N2E2_230 0.20 +1.4 292,731 + Pf6N2E2_230 0.22 +1.4 292,732 - Pf6N2E2_230 0.22 +0.1 292,732 - Pf6N2E2_230 0.22 +0.7 292,789 - Pf6N2E2_230 0.26 -0.6 292,789 - Pf6N2E2_230 0.26 +0.3 292,821 + Pf6N2E2_230 0.28 -0.3 292,822 - Pf6N2E2_230 0.28 -0.5 292,822 - Pf6N2E2_230 0.28 +1.4 292,916 + Pf6N2E2_230 0.34 -0.2 292,916 + Pf6N2E2_230 0.34 +1.4 292,917 - Pf6N2E2_230 0.34 +0.8 292,920 + Pf6N2E2_230 0.35 -0.0 292,921 - Pf6N2E2_230 0.35 +0.4 292,944 + Pf6N2E2_230 0.36 +0.4 292,944 + Pf6N2E2_230 0.36 +0.4 292,945 - Pf6N2E2_230 0.36 +1.0 292,945 - Pf6N2E2_230 0.36 +0.4 292,945 - Pf6N2E2_230 0.36 +0.6 292,945 - Pf6N2E2_230 0.36 +0.6 292,980 + Pf6N2E2_230 0.39 +0.8 292,980 + Pf6N2E2_230 0.39 -0.2 292,980 + Pf6N2E2_230 0.39 +1.0 292,980 + Pf6N2E2_230 0.39 -0.1 292,980 + Pf6N2E2_230 0.39 +0.0 292,981 - Pf6N2E2_230 0.39 +1.4 292,981 - Pf6N2E2_230 0.39 +1.4 293,014 + Pf6N2E2_230 0.41 +0.7 293,015 - Pf6N2E2_230 0.41 +0.8 293,227 - Pf6N2E2_230 0.56 -0.2 293,284 - Pf6N2E2_230 0.60 +0.2 293,319 + Pf6N2E2_230 0.62 +1.4 293,319 + Pf6N2E2_230 0.62 +1.9 293,547 + Pf6N2E2_230 0.78 +0.9 293,548 - Pf6N2E2_230 0.78 +1.4 293,548 - Pf6N2E2_230 0.78 -0.5 293,820 + +0.2 293,989 + -0.8 293,996 + -0.3 293,997 - -0.0 293,997 - +0.2 294,151 + Pf6N2E2_231 0.21 -0.3 294,152 - Pf6N2E2_231 0.21 +0.1 294,152 - Pf6N2E2_231 0.21 -1.6 294,155 + Pf6N2E2_231 0.21 -0.4 294,180 + Pf6N2E2_231 0.24 -0.3 294,181 - Pf6N2E2_231 0.24 +1.4 294,227 + Pf6N2E2_231 0.27 +1.4 294,227 + Pf6N2E2_231 0.27 +1.4 294,287 - Pf6N2E2_231 0.33 -0.2 294,413 + Pf6N2E2_231 0.43 -0.1 294,413 + Pf6N2E2_231 0.43 -1.3 294,413 + Pf6N2E2_231 0.43 -1.1 294,414 - Pf6N2E2_231 0.43 +0.0
Or see this region's nucleotide sequence