Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_1411 and Pf6N2E2_1412 overlap by 15 nucleotides Pf6N2E2_1412 and Pf6N2E2_1413 are separated by 53 nucleotides Pf6N2E2_1413 and Pf6N2E2_1414 are separated by 158 nucleotides Pf6N2E2_1414 and Pf6N2E2_1415 overlap by 18 nucleotides 
        Pf6N2E2_1411 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 1,656,648 to 1,657,742 
        _1411 
         
        
        Pf6N2E2_1412 - hypothetical protein, at 1,657,728 to 1,658,063 
        _1412 
         
        
        Pf6N2E2_1413 - putative membrane protein, at 1,658,117 to 1,658,560 
        _1413 
         
        
        Pf6N2E2_1414 - hypothetical protein, at 1,658,719 to 1,658,901 
        _1414 
         
        
        Pf6N2E2_1415 - mannuronan C-5-epimerase, putative, at 1,658,884 to 1,664,496 
        _1415 
         Position (kb) 1657 
1658 
1659 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 at 1656.988 kb on + strand, within Pf6N2E2_1411 at 1656.989 kb on - strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.015 kb on - strand at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.037 kb on + strand, within Pf6N2E2_1411 at 1657.086 kb on - strand, within Pf6N2E2_1411 at 1657.174 kb on + strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.300 kb on + strand, within Pf6N2E2_1411 at 1657.301 kb on - strand, within Pf6N2E2_1411 at 1657.615 kb on + strand, within Pf6N2E2_1411 at 1657.616 kb on - strand, within Pf6N2E2_1411 at 1657.616 kb on - strand, within Pf6N2E2_1411 at 1657.753 kb on + strand at 1657.754 kb on - strand at 1657.754 kb on - strand at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.941 kb on - strand, within Pf6N2E2_1412 at 1658.130 kb on + strand at 1658.130 kb on + strand at 1658.130 kb on + strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.683 kb on - strand at 1658.817 kb on + strand, within Pf6N2E2_1414 at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1659.014 kb on - strand at 1659.014 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 1,656,988 +  Pf6N2E2_1411 0.31  -2.9 1,656,989 -  Pf6N2E2_1411 0.31  +1.4 1,657,014 +  Pf6N2E2_1411 0.33  -2.0 1,657,014 +  Pf6N2E2_1411 0.33  -0.6 1,657,014 +  Pf6N2E2_1411 0.33  +1.4 1,657,015 -  +1.4 1,657,015 -  Pf6N2E2_1411 0.34  -1.4 1,657,015 -  Pf6N2E2_1411 0.34  -1.0 1,657,015 -  Pf6N2E2_1411 0.34  -2.6 1,657,019 -  Pf6N2E2_1411 0.34  -4.1 1,657,019 -  Pf6N2E2_1411 0.34  -2.1 1,657,019 -  Pf6N2E2_1411 0.34  -2.2 1,657,037 +  Pf6N2E2_1411 0.36  -3.7 1,657,086 -  Pf6N2E2_1411 0.40  +1.4 1,657,174 +  Pf6N2E2_1411 0.48  -3.2 1,657,175 -  Pf6N2E2_1411 0.48  -1.6 1,657,175 -  Pf6N2E2_1411 0.48  +1.4 1,657,175 -  Pf6N2E2_1411 0.48  -2.6 1,657,175 -  Pf6N2E2_1411 0.48  -0.9 1,657,175 -  Pf6N2E2_1411 0.48  -0.6 1,657,175 -  Pf6N2E2_1411 0.48  -1.4 1,657,175 -  Pf6N2E2_1411 0.48  -1.2 1,657,300 +  Pf6N2E2_1411 0.60  -3.2 1,657,301 -  Pf6N2E2_1411 0.60  -1.6 1,657,615 +  Pf6N2E2_1411 0.88  +1.4 1,657,616 -  Pf6N2E2_1411 0.88  -1.7 1,657,616 -  Pf6N2E2_1411 0.88  -2.8 1,657,753 +  -3.0 1,657,754 -  -1.1 1,657,754 -  -2.0 1,657,935 -  Pf6N2E2_1412 0.62  -3.4 1,657,935 -  Pf6N2E2_1412 0.62  -0.2 1,657,935 -  Pf6N2E2_1412 0.62  -2.5 1,657,935 -  Pf6N2E2_1412 0.62  -0.8 1,657,941 -  Pf6N2E2_1412 0.63  -1.9 1,658,130 +  -0.4 1,658,130 +  -1.3 1,658,130 +  -0.7 1,658,131 -  +0.2 1,658,131 -  -0.1 1,658,131 -  +1.1 1,658,131 -  +0.9 1,658,683 -  +0.4 1,658,817 +  Pf6N2E2_1414 0.54  +0.9 1,658,995 +  +1.4 1,658,995 +  +0.1 1,658,995 +  +0.1 1,658,995 +  +0.4 1,658,995 +  +0.4 1,658,995 +  +0.4 1,658,995 +  +1.4 1,658,996 -  -0.6 1,658,996 -  -1.6 1,658,996 -  +0.5 1,658,996 -  +1.4 1,658,996 -  -0.6 1,659,014 -  +0.4 1,659,014 -  +0.3 
Or see this region's nucleotide sequence