Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1031 and Pf6N2E2_1032 are separated by 1 nucleotides Pf6N2E2_1032 and Pf6N2E2_1033 are separated by 25 nucleotides
Pf6N2E2_1031 - Alkanesulfonates transport system permease protein, at 1,205,278 to 1,206,222
_1031
Pf6N2E2_1032 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 1,206,224 to 1,207,036
_1032
Pf6N2E2_1033 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,207,062 to 1,208,087
_1033
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1205.320 kb on + strand at 1205.321 kb on - strand at 1205.655 kb on - strand, within Pf6N2E2_1031 at 1205.655 kb on - strand, within Pf6N2E2_1031 at 1205.860 kb on + strand, within Pf6N2E2_1031 at 1205.860 kb on + strand, within Pf6N2E2_1031 at 1205.887 kb on - strand, within Pf6N2E2_1031 at 1206.032 kb on + strand, within Pf6N2E2_1031 at 1206.133 kb on + strand at 1206.133 kb on + strand at 1206.133 kb on + strand at 1206.133 kb on + strand at 1206.134 kb on - strand at 1206.134 kb on - strand at 1206.134 kb on - strand at 1206.134 kb on - strand at 1206.134 kb on - strand at 1206.224 kb on - strand at 1206.757 kb on - strand, within Pf6N2E2_1032 at 1207.000 kb on + strand at 1207.006 kb on + strand at 1207.006 kb on + strand at 1207.007 kb on - strand at 1207.007 kb on - strand at 1207.035 kb on - strand at 1207.035 kb on - strand at 1207.263 kb on + strand, within Pf6N2E2_1033 at 1207.264 kb on - strand, within Pf6N2E2_1033 at 1207.264 kb on - strand, within Pf6N2E2_1033 at 1207.528 kb on - strand, within Pf6N2E2_1033 at 1207.846 kb on - strand, within Pf6N2E2_1033 at 1207.927 kb on + strand, within Pf6N2E2_1033
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C remove 1,205,320 + -1.0 1,205,321 - -0.6 1,205,655 - Pf6N2E2_1031 0.40 +0.1 1,205,655 - Pf6N2E2_1031 0.40 +1.4 1,205,860 + Pf6N2E2_1031 0.62 +1.4 1,205,860 + Pf6N2E2_1031 0.62 +0.2 1,205,887 - Pf6N2E2_1031 0.64 +0.5 1,206,032 + Pf6N2E2_1031 0.80 +1.4 1,206,133 + -0.4 1,206,133 + +0.8 1,206,133 + -0.3 1,206,133 + +0.3 1,206,134 - -0.6 1,206,134 - +0.2 1,206,134 - +2.4 1,206,134 - +1.4 1,206,134 - +0.5 1,206,224 - +2.4 1,206,757 - Pf6N2E2_1032 0.66 -1.1 1,207,000 + +1.5 1,207,006 + -0.5 1,207,006 + -1.4 1,207,007 - -0.1 1,207,007 - +0.5 1,207,035 - +0.4 1,207,035 - -2.2 1,207,263 + Pf6N2E2_1033 0.20 -0.2 1,207,264 - Pf6N2E2_1033 0.20 +0.3 1,207,264 - Pf6N2E2_1033 0.20 -0.1 1,207,528 - Pf6N2E2_1033 0.45 +0.9 1,207,846 - Pf6N2E2_1033 0.76 +0.0 1,207,927 + Pf6N2E2_1033 0.84 +0.6
Or see this region's nucleotide sequence