Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_705 and Pf6N2E2_706 are separated by 198 nucleotides Pf6N2E2_706 and Pf6N2E2_707 overlap by 17 nucleotides Pf6N2E2_707 and Pf6N2E2_708 are separated by 45 nucleotides
Pf6N2E2_705 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 809,043 to 810,722
_705
Pf6N2E2_706 - Nitric oxide reductase activation protein NorQ, at 810,921 to 811,724
_706
Pf6N2E2_707 - Nitric oxide reductase activation protein NorE, at 811,708 to 812,292
_707
Pf6N2E2_708 - hypothetical protein, at 812,338 to 812,562
_708
Position (kb) 810
811
812 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.960 kb on - strand, within Pf6N2E2_705 at 810.100 kb on + strand, within Pf6N2E2_705 at 810.245 kb on + strand, within Pf6N2E2_705 at 810.430 kb on + strand, within Pf6N2E2_705 at 810.430 kb on + strand, within Pf6N2E2_705 at 810.430 kb on + strand, within Pf6N2E2_705 at 810.430 kb on + strand, within Pf6N2E2_705 at 810.431 kb on - strand, within Pf6N2E2_705 at 810.431 kb on - strand, within Pf6N2E2_705 at 810.451 kb on + strand, within Pf6N2E2_705 at 810.452 kb on - strand, within Pf6N2E2_705 at 810.671 kb on + strand at 810.672 kb on - strand at 810.758 kb on + strand at 810.758 kb on + strand at 810.758 kb on + strand at 810.758 kb on + strand at 810.759 kb on - strand at 810.759 kb on - strand at 810.759 kb on - strand at 810.963 kb on + strand at 810.963 kb on + strand at 810.963 kb on + strand at 810.963 kb on + strand at 810.963 kb on + strand at 810.964 kb on - strand at 810.964 kb on - strand at 811.102 kb on - strand, within Pf6N2E2_706 at 811.102 kb on - strand, within Pf6N2E2_706 at 811.102 kb on - strand, within Pf6N2E2_706 at 811.102 kb on - strand, within Pf6N2E2_706 at 811.221 kb on - strand, within Pf6N2E2_706 at 811.221 kb on - strand, within Pf6N2E2_706 at 811.228 kb on - strand, within Pf6N2E2_706 at 811.305 kb on + strand, within Pf6N2E2_706 at 811.306 kb on - strand, within Pf6N2E2_706 at 811.306 kb on - strand, within Pf6N2E2_706 at 811.365 kb on + strand, within Pf6N2E2_706 at 811.365 kb on + strand, within Pf6N2E2_706 at 811.365 kb on + strand, within Pf6N2E2_706 at 811.365 kb on + strand, within Pf6N2E2_706 at 811.366 kb on - strand, within Pf6N2E2_706 at 811.366 kb on - strand, within Pf6N2E2_706 at 811.469 kb on + strand, within Pf6N2E2_706 at 811.840 kb on + strand, within Pf6N2E2_707 at 811.841 kb on - strand, within Pf6N2E2_707 at 811.841 kb on - strand, within Pf6N2E2_707 at 812.017 kb on + strand, within Pf6N2E2_707 at 812.017 kb on + strand, within Pf6N2E2_707 at 812.018 kb on - strand, within Pf6N2E2_707 at 812.041 kb on + strand, within Pf6N2E2_707 at 812.041 kb on + strand, within Pf6N2E2_707 at 812.041 kb on + strand, within Pf6N2E2_707 at 812.042 kb on - strand, within Pf6N2E2_707 at 812.098 kb on + strand, within Pf6N2E2_707 at 812.098 kb on + strand, within Pf6N2E2_707 at 812.098 kb on + strand, within Pf6N2E2_707 at 812.099 kb on - strand, within Pf6N2E2_707 at 812.099 kb on - strand, within Pf6N2E2_707 at 812.230 kb on + strand, within Pf6N2E2_707 at 812.230 kb on + strand, within Pf6N2E2_707 at 812.278 kb on + strand at 812.279 kb on - strand at 812.279 kb on - strand at 812.279 kb on - strand at 812.560 kb on + strand at 812.561 kb on - strand at 812.561 kb on - strand at 812.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 809,959 + Pf6N2E2_705 0.55 -0.9 809,959 + Pf6N2E2_705 0.55 -0.2 809,959 + Pf6N2E2_705 0.55 +1.6 809,959 + Pf6N2E2_705 0.55 -1.4 809,960 - Pf6N2E2_705 0.55 -1.4 810,100 + Pf6N2E2_705 0.63 -1.3 810,245 + Pf6N2E2_705 0.72 -1.2 810,430 + Pf6N2E2_705 0.83 -0.5 810,430 + Pf6N2E2_705 0.83 +0.1 810,430 + Pf6N2E2_705 0.83 -1.5 810,430 + Pf6N2E2_705 0.83 +0.0 810,431 - Pf6N2E2_705 0.83 -0.4 810,431 - Pf6N2E2_705 0.83 -2.8 810,451 + Pf6N2E2_705 0.84 -1.3 810,452 - Pf6N2E2_705 0.84 +0.6 810,671 + -2.1 810,672 - +0.0 810,758 + +0.6 810,758 + +1.6 810,758 + -1.0 810,758 + -2.5 810,759 - +0.3 810,759 - +2.2 810,759 - +0.0 810,963 + -2.9 810,963 + +0.0 810,963 + -2.1 810,963 + -3.1 810,963 + -1.8 810,964 - -2.8 810,964 - -3.1 811,102 - Pf6N2E2_706 0.23 +1.6 811,102 - Pf6N2E2_706 0.23 -2.7 811,102 - Pf6N2E2_706 0.23 -3.3 811,102 - Pf6N2E2_706 0.23 -0.4 811,221 - Pf6N2E2_706 0.37 +1.6 811,221 - Pf6N2E2_706 0.37 -2.7 811,228 - Pf6N2E2_706 0.38 -2.5 811,305 + Pf6N2E2_706 0.48 +1.0 811,306 - Pf6N2E2_706 0.48 +0.6 811,306 - Pf6N2E2_706 0.48 -2.1 811,365 + Pf6N2E2_706 0.55 -1.2 811,365 + Pf6N2E2_706 0.55 -0.9 811,365 + Pf6N2E2_706 0.55 -0.5 811,365 + Pf6N2E2_706 0.55 -3.1 811,366 - Pf6N2E2_706 0.55 -2.9 811,366 - Pf6N2E2_706 0.55 -1.2 811,469 + Pf6N2E2_706 0.68 -2.6 811,840 + Pf6N2E2_707 0.23 -0.3 811,841 - Pf6N2E2_707 0.23 +0.8 811,841 - Pf6N2E2_707 0.23 +0.7 812,017 + Pf6N2E2_707 0.53 +1.5 812,017 + Pf6N2E2_707 0.53 -0.0 812,018 - Pf6N2E2_707 0.53 +0.8 812,041 + Pf6N2E2_707 0.57 -0.7 812,041 + Pf6N2E2_707 0.57 -0.4 812,041 + Pf6N2E2_707 0.57 -0.1 812,042 - Pf6N2E2_707 0.57 +0.2 812,098 + Pf6N2E2_707 0.67 -0.6 812,098 + Pf6N2E2_707 0.67 -0.7 812,098 + Pf6N2E2_707 0.67 +1.4 812,099 - Pf6N2E2_707 0.67 -0.8 812,099 - Pf6N2E2_707 0.67 -0.3 812,230 + Pf6N2E2_707 0.89 -0.1 812,230 + Pf6N2E2_707 0.89 +0.2 812,278 + -2.1 812,279 - +0.6 812,279 - -1.3 812,279 - -0.1 812,560 + +0.1 812,561 - +0.6 812,561 - +0.2 812,614 - +0.2
Or see this region's nucleotide sequence