Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5998 and Pf6N2E2_5999 are separated by 532 nucleotides Pf6N2E2_5999 and Pf6N2E2_6000 are separated by 26 nucleotides
Pf6N2E2_5998 - Cytochrome c family protein, at 6,814,123 to 6,814,935
_5998
Pf6N2E2_5999 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 6,815,468 to 6,816,745
_5999
Pf6N2E2_6000 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 6,816,772 to 6,818,916
_6000
Position (kb)
6815
6816
6817 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6814.553 kb on + strand, within Pf6N2E2_5998 at 6814.553 kb on + strand, within Pf6N2E2_5998 at 6814.553 kb on + strand, within Pf6N2E2_5998 at 6814.732 kb on + strand, within Pf6N2E2_5998 at 6814.733 kb on - strand, within Pf6N2E2_5998 at 6814.733 kb on - strand, within Pf6N2E2_5998 at 6814.775 kb on + strand, within Pf6N2E2_5998 at 6814.775 kb on + strand, within Pf6N2E2_5998 at 6814.776 kb on - strand, within Pf6N2E2_5998 at 6814.792 kb on + strand, within Pf6N2E2_5998 at 6814.792 kb on + strand, within Pf6N2E2_5998 at 6814.792 kb on + strand, within Pf6N2E2_5998 at 6814.792 kb on + strand, within Pf6N2E2_5998 at 6814.792 kb on + strand, within Pf6N2E2_5998 at 6814.792 kb on + strand, within Pf6N2E2_5998 at 6814.793 kb on - strand, within Pf6N2E2_5998 at 6814.793 kb on - strand, within Pf6N2E2_5998 at 6814.886 kb on + strand at 6814.923 kb on + strand at 6814.923 kb on + strand at 6814.923 kb on + strand at 6814.923 kb on + strand at 6814.923 kb on + strand at 6814.923 kb on + strand at 6814.923 kb on + strand at 6814.923 kb on + strand at 6814.924 kb on - strand at 6814.924 kb on - strand at 6814.924 kb on - strand at 6815.026 kb on + strand at 6815.026 kb on + strand at 6815.026 kb on + strand at 6815.026 kb on + strand at 6815.026 kb on + strand at 6815.026 kb on + strand at 6815.027 kb on - strand at 6815.027 kb on - strand at 6815.027 kb on - strand at 6815.027 kb on - strand at 6815.027 kb on - strand at 6815.027 kb on - strand at 6815.027 kb on - strand at 6815.078 kb on + strand at 6815.079 kb on - strand at 6815.168 kb on + strand at 6815.169 kb on - strand at 6815.169 kb on - strand at 6815.174 kb on + strand at 6815.174 kb on + strand at 6815.174 kb on + strand at 6815.174 kb on + strand at 6815.175 kb on - strand at 6815.175 kb on - strand at 6815.181 kb on + strand at 6815.265 kb on + strand at 6815.266 kb on - strand at 6815.266 kb on - strand at 6815.266 kb on - strand at 6815.266 kb on - strand at 6815.266 kb on - strand at 6815.266 kb on - strand at 6815.274 kb on + strand at 6815.275 kb on - strand at 6815.275 kb on - strand at 6815.279 kb on + strand at 6815.315 kb on + strand at 6815.315 kb on + strand at 6815.316 kb on - strand at 6815.316 kb on - strand at 6815.386 kb on - strand at 6815.386 kb on - strand at 6815.497 kb on + strand at 6815.497 kb on + strand at 6815.498 kb on - strand at 6815.498 kb on - strand at 6815.518 kb on - strand at 6815.568 kb on + strand at 6815.568 kb on + strand at 6815.569 kb on - strand at 6815.569 kb on - strand at 6816.100 kb on + strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.258 kb on - strand, within Pf6N2E2_5999 at 6816.259 kb on + strand, within Pf6N2E2_5999 at 6816.260 kb on - strand, within Pf6N2E2_5999 at 6816.643 kb on + strand at 6816.868 kb on + strand at 6816.869 kb on - strand at 6816.869 kb on - strand at 6816.869 kb on - strand at 6817.056 kb on + strand, within Pf6N2E2_6000 at 6817.347 kb on + strand, within Pf6N2E2_6000 at 6817.601 kb on - strand, within Pf6N2E2_6000 at 6817.711 kb on + strand, within Pf6N2E2_6000 at 6817.712 kb on - strand, within Pf6N2E2_6000 at 6817.712 kb on - strand, within Pf6N2E2_6000
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 6,814,553 + Pf6N2E2_5998 0.53 -1.1 6,814,553 + Pf6N2E2_5998 0.53 -0.5 6,814,553 + Pf6N2E2_5998 0.53 +0.0 6,814,732 + Pf6N2E2_5998 0.75 +0.0 6,814,733 - Pf6N2E2_5998 0.75 -0.1 6,814,733 - Pf6N2E2_5998 0.75 -0.3 6,814,775 + Pf6N2E2_5998 0.80 -0.2 6,814,775 + Pf6N2E2_5998 0.80 +0.5 6,814,776 - Pf6N2E2_5998 0.80 +0.6 6,814,792 + Pf6N2E2_5998 0.82 +0.4 6,814,792 + Pf6N2E2_5998 0.82 -0.1 6,814,792 + Pf6N2E2_5998 0.82 +0.3 6,814,792 + Pf6N2E2_5998 0.82 +0.6 6,814,792 + Pf6N2E2_5998 0.82 -0.4 6,814,792 + Pf6N2E2_5998 0.82 +0.1 6,814,793 - Pf6N2E2_5998 0.82 +0.2 6,814,793 - Pf6N2E2_5998 0.82 +0.3 6,814,886 + +0.4 6,814,923 + +0.3 6,814,923 + -0.1 6,814,923 + +0.2 6,814,923 + -0.3 6,814,923 + -0.1 6,814,923 + -0.8 6,814,923 + -0.7 6,814,923 + -0.3 6,814,924 - +0.4 6,814,924 - +0.2 6,814,924 - +0.2 6,815,026 + -0.8 6,815,026 + -0.7 6,815,026 + +1.0 6,815,026 + -1.4 6,815,026 + -1.1 6,815,026 + +0.4 6,815,027 - -0.9 6,815,027 - +0.2 6,815,027 - +0.3 6,815,027 - +0.9 6,815,027 - -0.1 6,815,027 - -0.9 6,815,027 - +0.5 6,815,078 + -0.5 6,815,079 - -0.5 6,815,168 + -0.2 6,815,169 - +1.0 6,815,169 - +0.9 6,815,174 + +0.3 6,815,174 + +0.3 6,815,174 + +0.5 6,815,174 + +0.4 6,815,175 - +0.5 6,815,175 - -2.9 6,815,181 + -0.3 6,815,265 + -0.5 6,815,266 - +1.5 6,815,266 - -2.1 6,815,266 - -0.2 6,815,266 - +0.7 6,815,266 - -0.8 6,815,266 - -1.6 6,815,274 + -0.1 6,815,275 - -1.2 6,815,275 - +0.7 6,815,279 + +1.5 6,815,315 + +0.4 6,815,315 + +0.5 6,815,316 - -0.6 6,815,316 - +0.1 6,815,386 - -1.1 6,815,386 - +2.5 6,815,497 + +0.2 6,815,497 + +1.5 6,815,498 - +0.2 6,815,498 - +0.2 6,815,518 - +2.5 6,815,568 + -0.1 6,815,568 + +0.1 6,815,569 - -1.6 6,815,569 - -0.1 6,816,100 + Pf6N2E2_5999 0.49 +0.4 6,816,258 - Pf6N2E2_5999 0.62 +0.2 6,816,258 - Pf6N2E2_5999 0.62 -0.8 6,816,258 - Pf6N2E2_5999 0.62 -1.1 6,816,258 - Pf6N2E2_5999 0.62 -0.5 6,816,259 + Pf6N2E2_5999 0.62 -0.6 6,816,260 - Pf6N2E2_5999 0.62 +1.4 6,816,643 + +0.1 6,816,868 + +0.7 6,816,869 - +0.4 6,816,869 - -1.7 6,816,869 - -0.4 6,817,056 + Pf6N2E2_6000 0.13 +1.5 6,817,347 + Pf6N2E2_6000 0.27 -0.7 6,817,601 - Pf6N2E2_6000 0.39 -1.2 6,817,711 + Pf6N2E2_6000 0.44 -0.2 6,817,712 - Pf6N2E2_6000 0.44 -0.2 6,817,712 - Pf6N2E2_6000 0.44 -0.3
Or see this region's nucleotide sequence