Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5841 and Pf6N2E2_5842 are separated by 207 nucleotides Pf6N2E2_5842 and Pf6N2E2_5843 are separated by 354 nucleotides Pf6N2E2_5843 and Pf6N2E2_5844 are separated by 12 nucleotides
Pf6N2E2_5841 - GlcG protein, at 6,658,425 to 6,658,784
_5841
Pf6N2E2_5842 - Transcriptional regulator, TetR family, at 6,658,992 to 6,659,240
_5842
Pf6N2E2_5843 - hypothetical protein, at 6,659,595 to 6,659,717
_5843
Pf6N2E2_5844 - Membrane protein, putative, at 6,659,730 to 6,660,644
_5844
Position (kb)
6659
6660 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6658.729 kb on + strand, within Pf6N2E2_5841 at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.927 kb on - strand at 6658.927 kb on - strand at 6658.927 kb on - strand at 6659.050 kb on + strand, within Pf6N2E2_5842 at 6659.113 kb on + strand, within Pf6N2E2_5842 at 6659.113 kb on + strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.342 kb on + strand at 6659.342 kb on + strand at 6659.342 kb on + strand at 6659.342 kb on + strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.353 kb on + strand at 6659.353 kb on + strand at 6659.459 kb on + strand at 6659.459 kb on + strand at 6659.462 kb on + strand at 6659.462 kb on + strand at 6659.462 kb on + strand at 6659.463 kb on - strand at 6659.463 kb on - strand at 6659.465 kb on + strand at 6659.465 kb on + strand at 6659.465 kb on + strand at 6659.466 kb on - strand at 6659.599 kb on - strand at 6659.640 kb on + strand, within Pf6N2E2_5843 at 6659.640 kb on + strand, within Pf6N2E2_5843 at 6659.641 kb on - strand, within Pf6N2E2_5843 at 6659.709 kb on - strand at 6659.711 kb on + strand at 6659.711 kb on + strand at 6659.711 kb on + strand at 6659.712 kb on - strand at 6659.753 kb on - strand at 6659.753 kb on - strand at 6659.927 kb on + strand, within Pf6N2E2_5844 at 6660.019 kb on - strand, within Pf6N2E2_5844 at 6660.019 kb on - strand, within Pf6N2E2_5844 at 6660.170 kb on - strand, within Pf6N2E2_5844 at 6660.294 kb on + strand, within Pf6N2E2_5844 at 6660.295 kb on - strand, within Pf6N2E2_5844 at 6660.295 kb on - strand, within Pf6N2E2_5844 at 6660.295 kb on - strand, within Pf6N2E2_5844 at 6660.405 kb on + strand, within Pf6N2E2_5844 at 6660.405 kb on + strand, within Pf6N2E2_5844 at 6660.405 kb on + strand, within Pf6N2E2_5844 at 6660.405 kb on + strand, within Pf6N2E2_5844 at 6660.642 kb on + strand at 6660.642 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 6,658,729 + Pf6N2E2_5841 0.84 -0.1 6,658,926 + +0.6 6,658,926 + -0.7 6,658,926 + -0.4 6,658,926 + +1.5 6,658,926 + +1.5 6,658,926 + -0.1 6,658,926 + -0.1 6,658,927 - +1.5 6,658,927 - -0.5 6,658,927 - -1.7 6,659,050 + Pf6N2E2_5842 0.23 -0.2 6,659,113 + Pf6N2E2_5842 0.49 -1.8 6,659,113 + Pf6N2E2_5842 0.49 -0.2 6,659,114 - Pf6N2E2_5842 0.49 +0.5 6,659,114 - Pf6N2E2_5842 0.49 -0.3 6,659,114 - Pf6N2E2_5842 0.49 -2.0 6,659,114 - Pf6N2E2_5842 0.49 -0.2 6,659,114 - Pf6N2E2_5842 0.49 -0.8 6,659,342 + -0.8 6,659,342 + +0.6 6,659,342 + -0.8 6,659,342 + +1.5 6,659,343 - +1.5 6,659,343 - +0.1 6,659,343 - +1.5 6,659,343 - +1.5 6,659,343 - -0.1 6,659,343 - +1.5 6,659,343 - +0.6 6,659,353 + +0.4 6,659,353 + -0.4 6,659,459 + -1.3 6,659,459 + -1.8 6,659,462 + +3.1 6,659,462 + +1.5 6,659,462 + -0.1 6,659,463 - -0.1 6,659,463 - +1.5 6,659,465 + -3.0 6,659,465 + -0.8 6,659,465 + +0.5 6,659,466 - -0.1 6,659,599 - +0.1 6,659,640 + Pf6N2E2_5843 0.37 -0.1 6,659,640 + Pf6N2E2_5843 0.37 +1.5 6,659,641 - Pf6N2E2_5843 0.37 -1.4 6,659,709 - +0.2 6,659,711 + -0.1 6,659,711 + -0.5 6,659,711 + +1.0 6,659,712 - +1.5 6,659,753 - -0.6 6,659,753 - +0.5 6,659,927 + Pf6N2E2_5844 0.22 +0.5 6,660,019 - Pf6N2E2_5844 0.32 +0.3 6,660,019 - Pf6N2E2_5844 0.32 +0.6 6,660,170 - Pf6N2E2_5844 0.48 +0.0 6,660,294 + Pf6N2E2_5844 0.62 +0.3 6,660,295 - Pf6N2E2_5844 0.62 +0.2 6,660,295 - Pf6N2E2_5844 0.62 +0.6 6,660,295 - Pf6N2E2_5844 0.62 -1.8 6,660,405 + Pf6N2E2_5844 0.74 +0.9 6,660,405 + Pf6N2E2_5844 0.74 +0.0 6,660,405 + Pf6N2E2_5844 0.74 +0.9 6,660,405 + Pf6N2E2_5844 0.74 +0.2 6,660,642 + -0.0 6,660,642 + -2.3 6,660,709 + -0.3 6,660,709 + +0.8 6,660,709 + -0.8 6,660,709 + +2.0 6,660,709 + -1.1 6,660,709 + -0.3 6,660,709 + -0.2 6,660,709 + -0.6 6,660,710 - +0.4
Or see this region's nucleotide sequence