Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_5841 and Pf6N2E2_5842 are separated by 207 nucleotides Pf6N2E2_5842 and Pf6N2E2_5843 are separated by 354 nucleotides Pf6N2E2_5843 and Pf6N2E2_5844 are separated by 12 nucleotides 
        Pf6N2E2_5841 - GlcG protein, at 6,658,425 to 6,658,784 
        _5841 
         
        
        Pf6N2E2_5842 - Transcriptional regulator, TetR family, at 6,658,992 to 6,659,240 
        _5842 
         
        
        Pf6N2E2_5843 - hypothetical protein, at 6,659,595 to 6,659,717 
        _5843 
         
        
        Pf6N2E2_5844 - Membrane protein, putative, at 6,659,730 to 6,660,644 
        _5844 
         Position (kb) 6659 
6660 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 
3 at 6658.729 kb on + strand, within Pf6N2E2_5841 at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.926 kb on + strand at 6658.927 kb on - strand at 6658.927 kb on - strand at 6658.927 kb on - strand at 6659.050 kb on + strand, within Pf6N2E2_5842 at 6659.113 kb on + strand, within Pf6N2E2_5842 at 6659.113 kb on + strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.114 kb on - strand, within Pf6N2E2_5842 at 6659.342 kb on + strand at 6659.342 kb on + strand at 6659.342 kb on + strand at 6659.342 kb on + strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.343 kb on - strand at 6659.353 kb on + strand at 6659.353 kb on + strand at 6659.459 kb on + strand at 6659.459 kb on + strand at 6659.462 kb on + strand at 6659.462 kb on + strand at 6659.462 kb on + strand at 6659.463 kb on - strand at 6659.463 kb on - strand at 6659.465 kb on + strand at 6659.465 kb on + strand at 6659.465 kb on + strand at 6659.466 kb on - strand at 6659.599 kb on - strand at 6659.640 kb on + strand, within Pf6N2E2_5843 at 6659.640 kb on + strand, within Pf6N2E2_5843 at 6659.641 kb on - strand, within Pf6N2E2_5843 at 6659.709 kb on - strand at 6659.711 kb on + strand at 6659.711 kb on + strand at 6659.711 kb on + strand at 6659.712 kb on - strand at 6659.753 kb on - strand at 6659.753 kb on - strand at 6659.927 kb on + strand, within Pf6N2E2_5844 at 6660.019 kb on - strand, within Pf6N2E2_5844 at 6660.019 kb on - strand, within Pf6N2E2_5844 at 6660.170 kb on - strand, within Pf6N2E2_5844 at 6660.294 kb on + strand, within Pf6N2E2_5844 at 6660.295 kb on - strand, within Pf6N2E2_5844 at 6660.295 kb on - strand, within Pf6N2E2_5844 at 6660.295 kb on - strand, within Pf6N2E2_5844 at 6660.405 kb on + strand, within Pf6N2E2_5844 at 6660.405 kb on + strand, within Pf6N2E2_5844 at 6660.405 kb on + strand, within Pf6N2E2_5844 at 6660.405 kb on + strand, within Pf6N2E2_5844 at 6660.642 kb on + strand at 6660.642 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.710 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 6,658,729 +  Pf6N2E2_5841 0.84  -0.1 6,658,926 +  +0.6 6,658,926 +  -0.7 6,658,926 +  -0.4 6,658,926 +  +1.5 6,658,926 +  +1.5 6,658,926 +  -0.1 6,658,926 +  -0.1 6,658,927 -  +1.5 6,658,927 -  -0.5 6,658,927 -  -1.7 6,659,050 +  Pf6N2E2_5842 0.23  -0.2 6,659,113 +  Pf6N2E2_5842 0.49  -1.8 6,659,113 +  Pf6N2E2_5842 0.49  -0.2 6,659,114 -  Pf6N2E2_5842 0.49  +0.5 6,659,114 -  Pf6N2E2_5842 0.49  -0.3 6,659,114 -  Pf6N2E2_5842 0.49  -2.0 6,659,114 -  Pf6N2E2_5842 0.49  -0.2 6,659,114 -  Pf6N2E2_5842 0.49  -0.8 6,659,342 +  -0.8 6,659,342 +  +0.6 6,659,342 +  -0.8 6,659,342 +  +1.5 6,659,343 -  +1.5 6,659,343 -  +0.1 6,659,343 -  +1.5 6,659,343 -  +1.5 6,659,343 -  -0.1 6,659,343 -  +1.5 6,659,343 -  +0.6 6,659,353 +  +0.4 6,659,353 +  -0.4 6,659,459 +  -1.3 6,659,459 +  -1.8 6,659,462 +  +3.1 6,659,462 +  +1.5 6,659,462 +  -0.1 6,659,463 -  -0.1 6,659,463 -  +1.5 6,659,465 +  -3.0 6,659,465 +  -0.8 6,659,465 +  +0.5 6,659,466 -  -0.1 6,659,599 -  +0.1 6,659,640 +  Pf6N2E2_5843 0.37  -0.1 6,659,640 +  Pf6N2E2_5843 0.37  +1.5 6,659,641 -  Pf6N2E2_5843 0.37  -1.4 6,659,709 -  +0.2 6,659,711 +  -0.1 6,659,711 +  -0.5 6,659,711 +  +1.0 6,659,712 -  +1.5 6,659,753 -  -0.6 6,659,753 -  +0.5 6,659,927 +  Pf6N2E2_5844 0.22  +0.5 6,660,019 -  Pf6N2E2_5844 0.32  +0.3 6,660,019 -  Pf6N2E2_5844 0.32  +0.6 6,660,170 -  Pf6N2E2_5844 0.48  +0.0 6,660,294 +  Pf6N2E2_5844 0.62  +0.3 6,660,295 -  Pf6N2E2_5844 0.62  +0.2 6,660,295 -  Pf6N2E2_5844 0.62  +0.6 6,660,295 -  Pf6N2E2_5844 0.62  -1.8 6,660,405 +  Pf6N2E2_5844 0.74  +0.9 6,660,405 +  Pf6N2E2_5844 0.74  +0.0 6,660,405 +  Pf6N2E2_5844 0.74  +0.9 6,660,405 +  Pf6N2E2_5844 0.74  +0.2 6,660,642 +  -0.0 6,660,642 +  -2.3 6,660,709 +  -0.3 6,660,709 +  +0.8 6,660,709 +  -0.8 6,660,709 +  +2.0 6,660,709 +  -1.1 6,660,709 +  -0.3 6,660,709 +  -0.2 6,660,709 +  -0.6 6,660,710 -  +0.4 
Or see this region's nucleotide sequence