Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_5697 and Pf6N2E2_5698 are separated by 361 nucleotides Pf6N2E2_5698 and Pf6N2E2_5699 are separated by 875 nucleotides 
        Pf6N2E2_5697 - Flagellar hook protein FlgE, at 6,509,315 to 6,510,640 
        _5697 
         
        
        Pf6N2E2_5698 - Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1), at 6,511,002 to 6,513,893 
        _5698 
         
        
        Pf6N2E2_5699 - Transcriptional regulatory protein RstA, at 6,514,769 to 6,515,491 
        _5699 
         Position (kb) 6511 
6512 
6513 
6514 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 6510.166 kb on + strand, within Pf6N2E2_5697 at 6510.166 kb on + strand, within Pf6N2E2_5697 at 6510.166 kb on + strand, within Pf6N2E2_5697 at 6510.166 kb on + strand, within Pf6N2E2_5697 at 6510.167 kb on - strand, within Pf6N2E2_5697 at 6510.167 kb on - strand, within Pf6N2E2_5697 at 6510.172 kb on + strand, within Pf6N2E2_5697 at 6510.172 kb on + strand, within Pf6N2E2_5697 at 6510.172 kb on + strand, within Pf6N2E2_5697 at 6510.172 kb on + strand, within Pf6N2E2_5697 at 6510.173 kb on - strand, within Pf6N2E2_5697 at 6510.185 kb on - strand, within Pf6N2E2_5697 at 6510.205 kb on + strand, within Pf6N2E2_5697 at 6510.205 kb on + strand, within Pf6N2E2_5697 at 6510.205 kb on + strand, within Pf6N2E2_5697 at 6510.206 kb on - strand, within Pf6N2E2_5697 at 6510.208 kb on + strand, within Pf6N2E2_5697 at 6510.209 kb on - strand, within Pf6N2E2_5697 at 6510.209 kb on - strand, within Pf6N2E2_5697 at 6510.209 kb on - strand, within Pf6N2E2_5697 at 6510.209 kb on - strand, within Pf6N2E2_5697 at 6510.209 kb on - strand, within Pf6N2E2_5697 at 6510.296 kb on - strand, within Pf6N2E2_5697 at 6510.296 kb on - strand, within Pf6N2E2_5697 at 6510.296 kb on - strand, within Pf6N2E2_5697 at 6510.296 kb on - strand, within Pf6N2E2_5697 at 6510.296 kb on - strand, within Pf6N2E2_5697 at 6510.324 kb on + strand, within Pf6N2E2_5697 at 6510.324 kb on + strand, within Pf6N2E2_5697 at 6510.324 kb on + strand, within Pf6N2E2_5697 at 6510.324 kb on + strand, within Pf6N2E2_5697 at 6510.324 kb on + strand, within Pf6N2E2_5697 at 6510.324 kb on + strand, within Pf6N2E2_5697 at 6510.324 kb on + strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.325 kb on - strand, within Pf6N2E2_5697 at 6510.572 kb on + strand at 6510.572 kb on + strand at 6510.572 kb on + strand at 6510.572 kb on + strand at 6510.573 kb on - strand at 6510.573 kb on - strand at 6510.573 kb on - strand at 6510.573 kb on - strand at 6510.704 kb on - strand at 6510.749 kb on - strand at 6510.791 kb on + strand at 6510.791 kb on + strand at 6510.792 kb on - strand at 6510.792 kb on - strand at 6510.886 kb on + strand at 6510.886 kb on + strand at 6510.983 kb on - strand at 6510.983 kb on - strand at 6513.963 kb on - strand at 6513.963 kb on - strand at 6514.252 kb on - strand at 6514.252 kb on - strand at 6514.252 kb on - strand at 6514.258 kb on - strand at 6514.258 kb on - strand at 6514.317 kb on - strand at 6514.317 kb on - strand at 6514.317 kb on - strand at 6514.317 kb on - strand at 6514.317 kb on - strand at 6514.326 kb on + strand at 6514.382 kb on - strand at 6514.382 kb on - strand at 6514.382 kb on - strand at 6514.463 kb on + strand at 6514.463 kb on + strand at 6514.463 kb on + strand at 6514.464 kb on - strand at 6514.464 kb on - strand at 6514.464 kb on - strand at 6514.520 kb on - strand at 6514.521 kb on + strand at 6514.521 kb on + strand at 6514.521 kb on + strand at 6514.521 kb on + strand at 6514.521 kb on + strand at 6514.521 kb on - strand at 6514.522 kb on - strand at 6514.522 kb on - strand at 6514.522 kb on - strand at 6514.522 kb on - strand at 6514.522 kb on - strand at 6514.522 kb on - strand at 6514.522 kb on - strand at 6514.561 kb on + strand at 6514.562 kb on - strand at 6514.562 kb on - strand at 6514.562 kb on - strand at 6514.582 kb on + strand at 6514.744 kb on - strand at 6514.744 kb on - strand at 6514.744 kb on - strand at 6514.791 kb on + strand at 6514.792 kb on - strand at 6514.838 kb on + strand at 6514.838 kb on + strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 6,510,166 +  Pf6N2E2_5697 0.64  +0.8 6,510,166 +  Pf6N2E2_5697 0.64  -0.2 6,510,166 +  Pf6N2E2_5697 0.64  +1.4 6,510,166 +  Pf6N2E2_5697 0.64  -0.3 6,510,167 -  Pf6N2E2_5697 0.64  +0.5 6,510,167 -  Pf6N2E2_5697 0.64  +1.3 6,510,172 +  Pf6N2E2_5697 0.65  +0.9 6,510,172 +  Pf6N2E2_5697 0.65  +1.1 6,510,172 +  Pf6N2E2_5697 0.65  +1.0 6,510,172 +  Pf6N2E2_5697 0.65  +1.1 6,510,173 -  Pf6N2E2_5697 0.65  +1.0 6,510,185 -  Pf6N2E2_5697 0.66  +1.3 6,510,205 +  Pf6N2E2_5697 0.67  +1.5 6,510,205 +  Pf6N2E2_5697 0.67  +1.1 6,510,205 +  Pf6N2E2_5697 0.67  +0.6 6,510,206 -  Pf6N2E2_5697 0.67  +0.9 6,510,208 +  Pf6N2E2_5697 0.67  +0.5 6,510,209 -  Pf6N2E2_5697 0.67  +1.3 6,510,209 -  Pf6N2E2_5697 0.67  +1.0 6,510,209 -  Pf6N2E2_5697 0.67  +1.1 6,510,209 -  Pf6N2E2_5697 0.67  -0.1 6,510,209 -  Pf6N2E2_5697 0.67  +1.8 6,510,296 -  Pf6N2E2_5697 0.74  +1.1 6,510,296 -  Pf6N2E2_5697 0.74  +1.0 6,510,296 -  Pf6N2E2_5697 0.74  +1.1 6,510,296 -  Pf6N2E2_5697 0.74  +0.6 6,510,296 -  Pf6N2E2_5697 0.74  +1.3 6,510,324 +  Pf6N2E2_5697 0.76  +0.5 6,510,324 +  Pf6N2E2_5697 0.76  +1.2 6,510,324 +  Pf6N2E2_5697 0.76  +1.1 6,510,324 +  Pf6N2E2_5697 0.76  +1.1 6,510,324 +  Pf6N2E2_5697 0.76  +1.1 6,510,324 +  Pf6N2E2_5697 0.76  +0.6 6,510,324 +  Pf6N2E2_5697 0.76  +0.7 6,510,325 -  Pf6N2E2_5697 0.76  -0.6 6,510,325 -  Pf6N2E2_5697 0.76  +0.7 6,510,325 -  Pf6N2E2_5697 0.76  +1.5 6,510,325 -  Pf6N2E2_5697 0.76  +1.2 6,510,325 -  Pf6N2E2_5697 0.76  +1.0 6,510,325 -  Pf6N2E2_5697 0.76  +0.4 6,510,325 -  Pf6N2E2_5697 0.76  +1.0 6,510,325 -  Pf6N2E2_5697 0.76  +1.1 6,510,325 -  Pf6N2E2_5697 0.76  +0.0 6,510,325 -  Pf6N2E2_5697 0.76  -0.2 6,510,572 +  +0.6 6,510,572 +  +1.2 6,510,572 +  +0.9 6,510,572 +  +0.5 6,510,573 -  +1.3 6,510,573 -  +2.2 6,510,573 -  +0.4 6,510,573 -  +2.3 6,510,704 -  -1.0 6,510,749 -  -0.6 6,510,791 +  -1.6 6,510,791 +  -0.7 6,510,792 -  -0.1 6,510,792 -  -0.0 6,510,886 +  -1.8 6,510,886 +  -0.4 6,510,983 -  -0.4 6,510,983 -  -1.6 6,513,963 -  -0.5 6,513,963 -  -1.3 6,514,252 -  -0.1 6,514,252 -  +1.1 6,514,252 -  -1.3 6,514,258 -  -0.6 6,514,258 -  +0.3 6,514,317 -  -1.4 6,514,317 -  +0.6 6,514,317 -  +0.6 6,514,317 -  -0.1 6,514,317 -  -0.1 6,514,326 +  -1.1 6,514,382 -  -0.1 6,514,382 -  -0.5 6,514,382 -  -0.7 6,514,463 +  -0.1 6,514,463 +  -0.3 6,514,463 +  +0.4 6,514,464 -  -1.2 6,514,464 -  -0.7 6,514,464 -  +1.5 6,514,520 -  +1.5 6,514,521 +  -1.0 6,514,521 +  +1.5 6,514,521 +  -0.1 6,514,521 +  +0.2 6,514,521 +  -0.8 6,514,521 -  +2.5 6,514,522 -  -0.2 6,514,522 -  -0.1 6,514,522 -  +0.1 6,514,522 -  +0.5 6,514,522 -  -0.3 6,514,522 -  +1.5 6,514,522 -  -0.7 6,514,561 +  -0.6 6,514,562 -  +1.5 6,514,562 -  -0.8 6,514,562 -  +0.5 6,514,582 +  -0.6 6,514,744 -  -0.1 6,514,744 -  -0.2 6,514,744 -  -0.7 6,514,791 +  -0.4 6,514,792 -  +0.5 6,514,838 +  -0.0 6,514,838 +  +0.1 
Or see this region's nucleotide sequence