Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_561 and Pf6N2E2_562 overlap by 4 nucleotides Pf6N2E2_562 and Pf6N2E2_563 are separated by 379 nucleotides
Pf6N2E2_561 - Uncharacterized protein ImpA, at 634,545 to 635,627
_561
Pf6N2E2_562 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 635,624 to 636,577
_562
Pf6N2E2_563 - Serine transporter, at 636,957 to 638,237
_563
Position (kb) 635
636
637 Strain fitness (log2 ratio) -2
-1
0
1 at 634.823 kb on + strand, within Pf6N2E2_561 at 634.824 kb on - strand, within Pf6N2E2_561 at 635.241 kb on + strand, within Pf6N2E2_561 at 635.241 kb on + strand, within Pf6N2E2_561 at 635.242 kb on - strand, within Pf6N2E2_561 at 635.266 kb on - strand, within Pf6N2E2_561 at 635.266 kb on - strand, within Pf6N2E2_561 at 635.459 kb on - strand, within Pf6N2E2_561 at 635.459 kb on - strand, within Pf6N2E2_561 at 635.486 kb on + strand, within Pf6N2E2_561 at 635.708 kb on + strand at 635.708 kb on + strand at 636.036 kb on + strand, within Pf6N2E2_562 at 636.069 kb on + strand, within Pf6N2E2_562 at 636.070 kb on - strand, within Pf6N2E2_562 at 636.076 kb on + strand, within Pf6N2E2_562 at 636.222 kb on - strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.534 kb on + strand at 636.535 kb on - strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.591 kb on - strand at 636.591 kb on - strand at 636.612 kb on + strand at 636.613 kb on - strand at 636.613 kb on - strand at 636.627 kb on + strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.659 kb on - strand at 636.661 kb on + strand at 636.661 kb on + strand at 636.690 kb on + strand at 636.694 kb on + strand at 636.694 kb on + strand at 636.694 kb on + strand at 636.695 kb on - strand at 636.695 kb on - strand at 636.720 kb on + strand at 636.721 kb on - strand at 636.787 kb on + strand at 636.809 kb on + strand at 636.809 kb on + strand at 636.815 kb on + strand at 637.082 kb on + strand at 637.083 kb on - strand at 637.083 kb on - strand at 637.083 kb on - strand at 637.167 kb on + strand, within Pf6N2E2_563 at 637.167 kb on + strand, within Pf6N2E2_563 at 637.167 kb on + strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.281 kb on + strand, within Pf6N2E2_563 at 637.282 kb on - strand, within Pf6N2E2_563 at 637.282 kb on - strand, within Pf6N2E2_563 at 637.314 kb on + strand, within Pf6N2E2_563 at 637.314 kb on + strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.368 kb on - strand, within Pf6N2E2_563
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 634,823 + Pf6N2E2_561 0.26 -0.2 634,824 - Pf6N2E2_561 0.26 -1.3 635,241 + Pf6N2E2_561 0.64 -0.6 635,241 + Pf6N2E2_561 0.64 -1.1 635,242 - Pf6N2E2_561 0.64 -0.2 635,266 - Pf6N2E2_561 0.67 +1.2 635,266 - Pf6N2E2_561 0.67 +0.8 635,459 - Pf6N2E2_561 0.84 +0.2 635,459 - Pf6N2E2_561 0.84 -0.1 635,486 + Pf6N2E2_561 0.87 +0.6 635,708 + +0.9 635,708 + -0.9 636,036 + Pf6N2E2_562 0.43 +0.6 636,069 + Pf6N2E2_562 0.47 +1.0 636,070 - Pf6N2E2_562 0.47 -0.1 636,076 + Pf6N2E2_562 0.47 +0.8 636,222 - Pf6N2E2_562 0.63 -0.4 636,335 + Pf6N2E2_562 0.75 -0.4 636,335 + Pf6N2E2_562 0.75 -0.6 636,335 + Pf6N2E2_562 0.75 -0.2 636,335 + Pf6N2E2_562 0.75 -0.1 636,336 - Pf6N2E2_562 0.75 -1.1 636,336 - Pf6N2E2_562 0.75 -0.2 636,336 - Pf6N2E2_562 0.75 -0.9 636,534 + +0.1 636,535 - +1.6 636,590 + +0.1 636,590 + -0.3 636,590 + +0.0 636,590 + +1.6 636,590 + -0.5 636,591 - -0.2 636,591 - +0.9 636,612 + -0.7 636,613 - +0.3 636,613 - +1.4 636,627 + +0.7 636,628 - -1.5 636,628 - +0.2 636,628 - +0.3 636,628 - -0.1 636,658 + +0.8 636,658 + -2.1 636,658 + +0.1 636,658 + +0.5 636,658 + +1.4 636,659 - +1.6 636,661 + +0.1 636,661 + -1.2 636,690 + -0.8 636,694 + -0.4 636,694 + -0.2 636,694 + +0.6 636,695 - +1.6 636,695 - -2.4 636,720 + +0.5 636,721 - -0.3 636,787 + -1.1 636,809 + +0.6 636,809 + -0.1 636,815 + -0.0 637,082 + +1.1 637,083 - +1.6 637,083 - -0.2 637,083 - +0.6 637,167 + Pf6N2E2_563 0.16 -0.1 637,167 + Pf6N2E2_563 0.16 -0.8 637,167 + Pf6N2E2_563 0.16 -0.2 637,168 - Pf6N2E2_563 0.16 +1.3 637,168 - Pf6N2E2_563 0.16 -0.8 637,168 - Pf6N2E2_563 0.16 -0.6 637,168 - Pf6N2E2_563 0.16 -0.4 637,281 + Pf6N2E2_563 0.25 -0.0 637,282 - Pf6N2E2_563 0.25 -0.4 637,282 - Pf6N2E2_563 0.25 -2.0 637,314 + Pf6N2E2_563 0.28 -0.1 637,314 + Pf6N2E2_563 0.28 +0.2 637,315 - Pf6N2E2_563 0.28 -1.0 637,315 - Pf6N2E2_563 0.28 -0.3 637,315 - Pf6N2E2_563 0.28 -1.0 637,368 - Pf6N2E2_563 0.32 -0.0
Or see this region's nucleotide sequence