Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_531

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_530 and Pf6N2E2_531 are separated by 140 nucleotidesPf6N2E2_531 and Pf6N2E2_532 are separated by 484 nucleotidesPf6N2E2_532 and Pf6N2E2_533 are separated by 179 nucleotides Pf6N2E2_530 - Histidine transporter, periplasmic histidine-binding protein, at 599,368 to 600,378 _530 Pf6N2E2_531 - FIG00966599: hypothetical protein, at 600,519 to 600,956 _531 Pf6N2E2_532 - Na(+) H(+) antiporter subunit G, at 601,441 to 601,554 _532 Pf6N2E2_533 - Na(+) H(+) antiporter subunit G, at 601,734 to 602,087 _533 Position (kb) 600 601Strain fitness (log2 ratio) -2 -1 0 1at 599.569 kb on - strand, within Pf6N2E2_530at 599.725 kb on + strand, within Pf6N2E2_530at 599.726 kb on - strand, within Pf6N2E2_530at 599.803 kb on + strand, within Pf6N2E2_530at 599.926 kb on + strand, within Pf6N2E2_530at 599.926 kb on + strand, within Pf6N2E2_530at 599.926 kb on + strand, within Pf6N2E2_530at 599.926 kb on + strand, within Pf6N2E2_530at 599.926 kb on + strand, within Pf6N2E2_530at 599.927 kb on - strand, within Pf6N2E2_530at 599.927 kb on - strand, within Pf6N2E2_530at 599.927 kb on - strand, within Pf6N2E2_530at 600.010 kb on + strand, within Pf6N2E2_530at 600.010 kb on + strand, within Pf6N2E2_530at 600.010 kb on + strand, within Pf6N2E2_530at 600.011 kb on - strand, within Pf6N2E2_530at 600.013 kb on + strand, within Pf6N2E2_530at 600.013 kb on + strand, within Pf6N2E2_530at 600.014 kb on - strand, within Pf6N2E2_530at 600.357 kb on + strandat 600.357 kb on + strandat 600.358 kb on - strandat 600.358 kb on - strandat 600.358 kb on - strandat 600.358 kb on - strandat 600.358 kb on - strandat 600.376 kb on + strandat 600.377 kb on - strandat 600.449 kb on + strandat 600.449 kb on + strandat 600.482 kb on - strandat 600.578 kb on + strand, within Pf6N2E2_531at 600.578 kb on + strand, within Pf6N2E2_531at 600.578 kb on + strand, within Pf6N2E2_531at 600.578 kb on + strand, within Pf6N2E2_531at 600.578 kb on + strand, within Pf6N2E2_531at 600.578 kb on + strand, within Pf6N2E2_531at 600.578 kb on + strand, within Pf6N2E2_531at 600.578 kb on + strand, within Pf6N2E2_531at 600.582 kb on + strand, within Pf6N2E2_531at 600.582 kb on + strand, within Pf6N2E2_531at 600.582 kb on + strand, within Pf6N2E2_531at 600.582 kb on + strand, within Pf6N2E2_531at 600.582 kb on + strand, within Pf6N2E2_531at 600.582 kb on + strand, within Pf6N2E2_531at 600.583 kb on - strand, within Pf6N2E2_531at 600.583 kb on - strand, within Pf6N2E2_531at 600.583 kb on - strand, within Pf6N2E2_531at 600.583 kb on - strand, within Pf6N2E2_531at 601.169 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
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599,569 - Pf6N2E2_530 0.20 -0.2
599,725 + Pf6N2E2_530 0.35 +0.0
599,726 - Pf6N2E2_530 0.35 -0.0
599,803 + Pf6N2E2_530 0.43 -2.0
599,926 + Pf6N2E2_530 0.55 +0.2
599,926 + Pf6N2E2_530 0.55 +1.6
599,926 + Pf6N2E2_530 0.55 -0.0
599,926 + Pf6N2E2_530 0.55 -1.0
599,926 + Pf6N2E2_530 0.55 -1.8
599,927 - Pf6N2E2_530 0.55 -0.0
599,927 - Pf6N2E2_530 0.55 -0.1
599,927 - Pf6N2E2_530 0.55 +1.2
600,010 + Pf6N2E2_530 0.64 -0.3
600,010 + Pf6N2E2_530 0.64 +0.3
600,010 + Pf6N2E2_530 0.64 -0.0
600,011 - Pf6N2E2_530 0.64 -0.2
600,013 + Pf6N2E2_530 0.64 -0.8
600,013 + Pf6N2E2_530 0.64 -0.1
600,014 - Pf6N2E2_530 0.64 -1.0
600,357 + -0.1
600,357 + -0.3
600,358 - +0.4
600,358 - +1.2
600,358 - +0.1
600,358 - -2.3
600,358 - -0.6
600,376 + -0.5
600,377 - -0.8
600,449 + -0.2
600,449 + +0.8
600,482 - -0.9
600,578 + Pf6N2E2_531 0.13 -1.6
600,578 + Pf6N2E2_531 0.13 -1.6
600,578 + Pf6N2E2_531 0.13 -0.5
600,578 + Pf6N2E2_531 0.13 -0.4
600,578 + Pf6N2E2_531 0.13 +1.6
600,578 + Pf6N2E2_531 0.13 -0.8
600,578 + Pf6N2E2_531 0.13 -2.3
600,578 + Pf6N2E2_531 0.13 -1.0
600,582 + Pf6N2E2_531 0.14 -2.0
600,582 + Pf6N2E2_531 0.14 -1.6
600,582 + Pf6N2E2_531 0.14 +0.1
600,582 + Pf6N2E2_531 0.14 -1.3
600,582 + Pf6N2E2_531 0.14 -1.1
600,582 + Pf6N2E2_531 0.14 -1.0
600,583 - Pf6N2E2_531 0.15 -0.3
600,583 - Pf6N2E2_531 0.15 +1.6
600,583 - Pf6N2E2_531 0.15 +0.3
600,583 - Pf6N2E2_531 0.15 +1.6
601,169 + -0.1

Or see this region's nucleotide sequence