Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5082

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
5,857,983	+				+0.5
5,857,983	+				-1.7
5,857,983	+				-2.6
5,857,984	-				-0.5
5,857,984	-				-1.8
5,857,984	-				-0.5
5,857,984	-				+0.5
5,858,153	-				+0.5
5,858,200	+				+2.5
5,858,201	-				+0.2
5,858,243	+		Pf6N2E2_5081	0.12	-0.2
5,858,243	+		Pf6N2E2_5081	0.12	-0.3
5,858,269	+		Pf6N2E2_5081	0.18	-0.1
5,858,404	+		Pf6N2E2_5081	0.49	+0.1
5,858,450	+		Pf6N2E2_5081	0.59	-1.1
5,858,450	+		Pf6N2E2_5081	0.59	+0.5
5,858,640	+				+0.5
5,858,723	+				+1.0
5,858,752	-				+0.5
5,858,775	+				-0.5
5,858,803	+				+0.0
5,858,809	+				-0.3
5,858,893	+				-0.1
5,858,930	+				-0.1
5,860,266	+				+0.5
5,860,266	+				-1.1
5,860,267	-				+0.3
5,860,267	-				+1.3
5,860,503	-				+0.1
5,860,706	+				-0.4
5,860,707	-				-0.7
5,860,816	-		Pf6N2E2_5083	0.10	-0.4
5,860,872	+		Pf6N2E2_5083	0.12	+0.2
5,860,873	-		Pf6N2E2_5083	0.12	-0.3
5,861,147	+		Pf6N2E2_5083	0.19	+0.5
5,861,148	-		Pf6N2E2_5083	0.19	-0.5
5,861,150	+		Pf6N2E2_5083	0.19	-0.2
5,861,151	-		Pf6N2E2_5083	0.19	-1.1