Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_371 and Pf6N2E2_372 are separated by 225 nucleotides Pf6N2E2_372 and Pf6N2E2_373 are separated by 288 nucleotides
Pf6N2E2_371 - Histone acetyltransferase HPA2 and related acetyltransferases, at 438,498 to 439,355
_371
Pf6N2E2_372 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 439,581 to 440,507
_372
Pf6N2E2_373 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 440,796 to 442,088
_373
Position (kb) 439
440
441 Strain fitness (log2 ratio) -1
0
1
2
3 at 438.685 kb on - strand, within Pf6N2E2_371 at 438.984 kb on - strand, within Pf6N2E2_371 at 439.015 kb on + strand, within Pf6N2E2_371 at 439.015 kb on + strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.117 kb on + strand, within Pf6N2E2_371 at 439.118 kb on - strand, within Pf6N2E2_371 at 439.325 kb on - strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.331 kb on - strand at 439.331 kb on - strand at 439.331 kb on - strand at 439.373 kb on + strand at 439.415 kb on + strand at 439.416 kb on - strand at 439.416 kb on - strand at 439.526 kb on + strand at 439.526 kb on + strand at 439.526 kb on + strand at 439.527 kb on - strand at 439.527 kb on - strand at 439.585 kb on + strand at 439.585 kb on + strand at 439.586 kb on - strand at 439.589 kb on + strand at 439.590 kb on - strand at 439.710 kb on + strand, within Pf6N2E2_372 at 439.710 kb on + strand, within Pf6N2E2_372 at 439.710 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 440.067 kb on + strand, within Pf6N2E2_372 at 440.215 kb on + strand, within Pf6N2E2_372 at 440.274 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.384 kb on - strand, within Pf6N2E2_372 at 440.492 kb on + strand at 440.494 kb on + strand at 440.494 kb on + strand at 440.495 kb on - strand at 440.495 kb on - strand at 440.495 kb on - strand at 440.540 kb on + strand at 440.586 kb on - strand at 440.676 kb on + strand at 440.677 kb on - strand at 440.912 kb on - strand at 441.063 kb on + strand, within Pf6N2E2_373 at 441.114 kb on + strand, within Pf6N2E2_373 at 441.115 kb on - strand, within Pf6N2E2_373 at 441.117 kb on - strand, within Pf6N2E2_373 at 441.117 kb on - strand, within Pf6N2E2_373 at 441.270 kb on + strand, within Pf6N2E2_373 at 441.271 kb on - strand, within Pf6N2E2_373 at 441.330 kb on + strand, within Pf6N2E2_373 at 441.330 kb on + strand, within Pf6N2E2_373
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 438,685 - Pf6N2E2_371 0.22 -0.4 438,984 - Pf6N2E2_371 0.57 +0.5 439,015 + Pf6N2E2_371 0.60 -1.1 439,015 + Pf6N2E2_371 0.60 -1.2 439,016 - Pf6N2E2_371 0.60 -0.0 439,016 - Pf6N2E2_371 0.60 +0.1 439,016 - Pf6N2E2_371 0.60 -0.1 439,117 + Pf6N2E2_371 0.72 -0.1 439,118 - Pf6N2E2_371 0.72 +1.2 439,325 - -0.7 439,330 + -0.8 439,330 + -1.4 439,330 + -1.1 439,330 + +0.1 439,330 + +0.3 439,331 - +0.2 439,331 - -0.3 439,331 - -0.2 439,373 + +0.6 439,415 + -1.4 439,416 - +0.7 439,416 - -0.2 439,526 + +1.6 439,526 + +0.5 439,526 + -0.0 439,527 - +1.1 439,527 - +0.9 439,585 + +0.6 439,585 + -0.5 439,586 - +2.9 439,589 + -0.2 439,590 - +0.6 439,710 + Pf6N2E2_372 0.14 -0.5 439,710 + Pf6N2E2_372 0.14 -0.5 439,710 + Pf6N2E2_372 0.14 +1.1 439,938 + Pf6N2E2_372 0.39 +0.1 439,938 + Pf6N2E2_372 0.39 +0.3 439,938 + Pf6N2E2_372 0.39 -1.1 439,939 - Pf6N2E2_372 0.39 -0.0 439,939 - Pf6N2E2_372 0.39 +1.0 439,939 - Pf6N2E2_372 0.39 -0.0 439,939 - Pf6N2E2_372 0.39 +0.2 440,067 + Pf6N2E2_372 0.52 -0.2 440,215 + Pf6N2E2_372 0.68 +0.2 440,274 - Pf6N2E2_372 0.75 +0.4 440,384 - Pf6N2E2_372 0.87 -0.8 440,384 - Pf6N2E2_372 0.87 +0.9 440,384 - Pf6N2E2_372 0.87 -0.5 440,384 - Pf6N2E2_372 0.87 +1.0 440,492 + -0.9 440,494 + -0.9 440,494 + -0.3 440,495 - +0.6 440,495 - -0.0 440,495 - -0.0 440,540 + +1.1 440,586 - +0.1 440,676 + -0.0 440,677 - +0.5 440,912 - +1.0 441,063 + Pf6N2E2_373 0.21 +1.6 441,114 + Pf6N2E2_373 0.25 +0.1 441,115 - Pf6N2E2_373 0.25 -0.2 441,117 - Pf6N2E2_373 0.25 +0.5 441,117 - Pf6N2E2_373 0.25 +0.6 441,270 + Pf6N2E2_373 0.37 -0.2 441,271 - Pf6N2E2_373 0.37 +0.3 441,330 + Pf6N2E2_373 0.41 +0.2 441,330 + Pf6N2E2_373 0.41 -0.1
Or see this region's nucleotide sequence