Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3254 and Pf6N2E2_3255 are separated by 22 nucleotides Pf6N2E2_3255 and Pf6N2E2_3256 are separated by 223 nucleotides Pf6N2E2_3256 and Pf6N2E2_3257 overlap by 4 nucleotides
Pf6N2E2_3254 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 3,764,696 to 3,765,961
_3254
Pf6N2E2_3255 - YrbA protein, at 3,765,984 to 3,766,223
_3255
Pf6N2E2_3256 - Uncharacterized protein YrbB, at 3,766,447 to 3,766,764
_3256
Pf6N2E2_3257 - Uncharacterized ABC transporter, auxiliary component YrbC, at 3,766,761 to 3,767,414
_3257
Position (kb)
3765
3766
3767 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.213 kb on - strand at 3766.317 kb on + strand at 3766.489 kb on + strand, within Pf6N2E2_3256 at 3766.966 kb on + strand, within Pf6N2E2_3257 at 3766.978 kb on + strand, within Pf6N2E2_3257 at 3766.979 kb on - strand, within Pf6N2E2_3257 at 3767.074 kb on + strand, within Pf6N2E2_3257 at 3767.074 kb on + strand, within Pf6N2E2_3257 at 3767.075 kb on - strand, within Pf6N2E2_3257 at 3767.167 kb on + strand, within Pf6N2E2_3257 at 3767.167 kb on + strand, within Pf6N2E2_3257 at 3767.167 kb on + strand, within Pf6N2E2_3257 at 3767.168 kb on - strand, within Pf6N2E2_3257
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 3,766,076 - Pf6N2E2_3255 0.38 +1.5 3,766,076 - Pf6N2E2_3255 0.38 -2.2 3,766,076 - Pf6N2E2_3255 0.38 +1.5 3,766,076 - Pf6N2E2_3255 0.38 -0.9 3,766,076 - Pf6N2E2_3255 0.38 -1.5 3,766,076 - Pf6N2E2_3255 0.38 -1.8 3,766,195 - Pf6N2E2_3255 0.88 -3.3 3,766,195 - Pf6N2E2_3255 0.88 -1.9 3,766,195 - Pf6N2E2_3255 0.88 -3.0 3,766,213 - -0.8 3,766,317 + -0.0 3,766,489 + Pf6N2E2_3256 0.13 +1.5 3,766,966 + Pf6N2E2_3257 0.31 -1.1 3,766,978 + Pf6N2E2_3257 0.33 -1.5 3,766,979 - Pf6N2E2_3257 0.33 -0.1 3,767,074 + Pf6N2E2_3257 0.48 +1.5 3,767,074 + Pf6N2E2_3257 0.48 -0.3 3,767,075 - Pf6N2E2_3257 0.48 +0.5 3,767,167 + Pf6N2E2_3257 0.62 +1.5 3,767,167 + Pf6N2E2_3257 0.62 +1.9 3,767,167 + Pf6N2E2_3257 0.62 +0.2 3,767,168 - Pf6N2E2_3257 0.62 +1.5
Or see this region's nucleotide sequence