Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_299 and Pf6N2E2_300 overlap by 4 nucleotides Pf6N2E2_300 and Pf6N2E2_301 are separated by 12 nucleotides Pf6N2E2_301 and Pf6N2E2_302 are separated by 0 nucleotides
Pf6N2E2_299 - FIG065221: Holliday junction DNA helicase, at 369,402 to 370,727
_299
Pf6N2E2_300 - CrcB protein, at 370,724 to 371,101
_300
Pf6N2E2_301 - Seryl-tRNA synthetase (EC 6.1.1.11), at 371,114 to 372,394
_301
Pf6N2E2_302 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 372,395 to 373,789
_302
Position (kb) 371
372
373 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 370.207 kb on - strand, within Pf6N2E2_299 at 370.248 kb on + strand, within Pf6N2E2_299 at 370.248 kb on + strand, within Pf6N2E2_299 at 370.249 kb on - strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.396 kb on - strand, within Pf6N2E2_299 at 370.396 kb on - strand, within Pf6N2E2_299 at 370.428 kb on + strand, within Pf6N2E2_299 at 370.429 kb on - strand, within Pf6N2E2_299 at 370.429 kb on - strand, within Pf6N2E2_299 at 370.552 kb on - strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.593 kb on + strand, within Pf6N2E2_299 at 370.594 kb on - strand, within Pf6N2E2_299 at 370.626 kb on + strand at 370.627 kb on - strand at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.846 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 371.099 kb on + strand at 372.401 kb on + strand at 372.587 kb on + strand, within Pf6N2E2_302 at 372.588 kb on - strand, within Pf6N2E2_302 at 373.049 kb on + strand, within Pf6N2E2_302 at 373.193 kb on + strand, within Pf6N2E2_302 at 373.193 kb on + strand, within Pf6N2E2_302
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B remove 370,207 - Pf6N2E2_299 0.61 +0.5 370,248 + Pf6N2E2_299 0.64 -1.0 370,248 + Pf6N2E2_299 0.64 -1.8 370,249 - Pf6N2E2_299 0.64 +0.1 370,395 + Pf6N2E2_299 0.75 -1.3 370,395 + Pf6N2E2_299 0.75 -0.5 370,395 + Pf6N2E2_299 0.75 -0.1 370,396 - Pf6N2E2_299 0.75 +1.2 370,396 - Pf6N2E2_299 0.75 -0.4 370,428 + Pf6N2E2_299 0.77 +0.6 370,429 - Pf6N2E2_299 0.77 +0.6 370,429 - Pf6N2E2_299 0.77 -1.3 370,552 - Pf6N2E2_299 0.87 +0.5 370,575 + Pf6N2E2_299 0.88 -0.8 370,575 + Pf6N2E2_299 0.88 -0.5 370,575 + Pf6N2E2_299 0.88 -0.4 370,593 + Pf6N2E2_299 0.90 -0.6 370,594 - Pf6N2E2_299 0.90 -0.2 370,626 + +0.0 370,627 - -0.6 370,826 + Pf6N2E2_300 0.27 +0.9 370,826 + Pf6N2E2_300 0.27 +0.6 370,826 + Pf6N2E2_300 0.27 -0.8 370,826 + Pf6N2E2_300 0.27 +0.2 370,846 + Pf6N2E2_300 0.32 -2.4 370,849 + Pf6N2E2_300 0.33 -1.4 370,849 + Pf6N2E2_300 0.33 -2.8 370,849 + Pf6N2E2_300 0.33 -2.4 371,099 + +0.2 372,401 + -1.9 372,587 + Pf6N2E2_302 0.14 -2.5 372,588 - Pf6N2E2_302 0.14 +1.6 373,049 + Pf6N2E2_302 0.47 -0.0 373,193 + Pf6N2E2_302 0.57 +1.6 373,193 + Pf6N2E2_302 0.57 +1.6
Or see this region's nucleotide sequence