Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_753

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_752 and Pf6N2E2_753 are separated by 157 nucleotidesPf6N2E2_753 and Pf6N2E2_754 are separated by 57 nucleotidesPf6N2E2_754 and Pf6N2E2_755 are separated by 24 nucleotides Pf6N2E2_752 - Mobile element protein, at 861,275 to 862,543 _752 Pf6N2E2_753 - AFG1-like ATPase, at 862,701 to 863,822 _753 Pf6N2E2_754 - Histone acetyltransferase HPA2 and related acetyltransferases, at 863,880 to 864,374 _754 Pf6N2E2_755 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 864,399 to 866,138 _755 Position (kb) 862 863 864Strain fitness (log2 ratio) -2 -1 0 1at 862.668 kb on - strandat 862.668 kb on - strandat 862.668 kb on - strandat 862.668 kb on - strandat 862.682 kb on + strandat 862.683 kb on - strandat 863.032 kb on + strand, within Pf6N2E2_753at 863.033 kb on - strand, within Pf6N2E2_753at 863.138 kb on + strand, within Pf6N2E2_753at 863.138 kb on + strand, within Pf6N2E2_753at 863.138 kb on + strand, within Pf6N2E2_753at 863.139 kb on - strand, within Pf6N2E2_753at 863.139 kb on - strand, within Pf6N2E2_753at 863.166 kb on + strand, within Pf6N2E2_753at 863.167 kb on - strand, within Pf6N2E2_753at 863.167 kb on - strand, within Pf6N2E2_753at 863.223 kb on + strand, within Pf6N2E2_753at 863.223 kb on + strand, within Pf6N2E2_753at 863.223 kb on + strand, within Pf6N2E2_753at 863.224 kb on - strand, within Pf6N2E2_753at 863.224 kb on - strand, within Pf6N2E2_753at 863.225 kb on + strand, within Pf6N2E2_753at 863.225 kb on + strand, within Pf6N2E2_753at 863.225 kb on + strand, within Pf6N2E2_753at 863.225 kb on + strand, within Pf6N2E2_753at 863.225 kb on + strand, within Pf6N2E2_753at 863.225 kb on + strand, within Pf6N2E2_753at 863.226 kb on - strand, within Pf6N2E2_753at 863.226 kb on - strand, within Pf6N2E2_753at 863.226 kb on - strand, within Pf6N2E2_753at 863.226 kb on - strand, within Pf6N2E2_753at 863.226 kb on - strand, within Pf6N2E2_753at 863.226 kb on - strand, within Pf6N2E2_753at 863.226 kb on - strand, within Pf6N2E2_753at 863.226 kb on - strand, within Pf6N2E2_753at 863.281 kb on + strand, within Pf6N2E2_753at 863.282 kb on - strand, within Pf6N2E2_753at 863.282 kb on - strand, within Pf6N2E2_753at 863.282 kb on - strand, within Pf6N2E2_753at 863.679 kb on + strand, within Pf6N2E2_753at 863.679 kb on + strand, within Pf6N2E2_753at 863.679 kb on + strand, within Pf6N2E2_753at 863.680 kb on - strand, within Pf6N2E2_753at 863.680 kb on - strand, within Pf6N2E2_753at 863.733 kb on + strandat 863.733 kb on + strandat 863.733 kb on + strandat 863.734 kb on - strandat 863.734 kb on - strandat 863.734 kb on - strandat 863.734 kb on - strandat 863.745 kb on + strandat 863.828 kb on - strandat 863.905 kb on + strandat 863.905 kb on + strandat 863.905 kb on + strandat 863.919 kb on + strandat 863.919 kb on + strandat 863.919 kb on + strandat 864.297 kb on + strand, within Pf6N2E2_754at 864.297 kb on + strand, within Pf6N2E2_754at 864.348 kb on - strandat 864.604 kb on + strand, within Pf6N2E2_755at 864.688 kb on + strand, within Pf6N2E2_755at 864.688 kb on + strand, within Pf6N2E2_755at 864.688 kb on + strand, within Pf6N2E2_755at 864.688 kb on + strand, within Pf6N2E2_755at 864.689 kb on - strand, within Pf6N2E2_755at 864.689 kb on - strand, within Pf6N2E2_755at 864.689 kb on - strand, within Pf6N2E2_755at 864.776 kb on - strand, within Pf6N2E2_755at 864.776 kb on - strand, within Pf6N2E2_755at 864.776 kb on - strand, within Pf6N2E2_755at 864.776 kb on - strand, within Pf6N2E2_755at 864.776 kb on - strand, within Pf6N2E2_755

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
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862,668 - +1.5
862,668 - +0.5
862,668 - +0.5
862,668 - -0.1
862,682 + +0.6
862,683 - +0.0
863,032 + Pf6N2E2_753 0.30 -0.1
863,033 - Pf6N2E2_753 0.30 -0.6
863,138 + Pf6N2E2_753 0.39 -0.7
863,138 + Pf6N2E2_753 0.39 -0.3
863,138 + Pf6N2E2_753 0.39 +0.9
863,139 - Pf6N2E2_753 0.39 +0.2
863,139 - Pf6N2E2_753 0.39 +0.5
863,166 + Pf6N2E2_753 0.41 -0.5
863,167 - Pf6N2E2_753 0.42 -1.2
863,167 - Pf6N2E2_753 0.42 -0.8
863,223 + Pf6N2E2_753 0.47 -1.2
863,223 + Pf6N2E2_753 0.47 -0.8
863,223 + Pf6N2E2_753 0.47 -0.4
863,224 - Pf6N2E2_753 0.47 -0.3
863,224 - Pf6N2E2_753 0.47 -0.3
863,225 + Pf6N2E2_753 0.47 -0.3
863,225 + Pf6N2E2_753 0.47 -2.2
863,225 + Pf6N2E2_753 0.47 -2.0
863,225 + Pf6N2E2_753 0.47 +0.3
863,225 + Pf6N2E2_753 0.47 -0.9
863,225 + Pf6N2E2_753 0.47 -0.9
863,226 - Pf6N2E2_753 0.47 -2.2
863,226 - Pf6N2E2_753 0.47 -0.0
863,226 - Pf6N2E2_753 0.47 +0.9
863,226 - Pf6N2E2_753 0.47 +1.5
863,226 - Pf6N2E2_753 0.47 +0.5
863,226 - Pf6N2E2_753 0.47 -1.4
863,226 - Pf6N2E2_753 0.47 -0.7
863,226 - Pf6N2E2_753 0.47 -0.1
863,281 + Pf6N2E2_753 0.52 -1.8
863,282 - Pf6N2E2_753 0.52 -1.0
863,282 - Pf6N2E2_753 0.52 -1.1
863,282 - Pf6N2E2_753 0.52 +0.5
863,679 + Pf6N2E2_753 0.87 -0.6
863,679 + Pf6N2E2_753 0.87 -1.3
863,679 + Pf6N2E2_753 0.87 +0.1
863,680 - Pf6N2E2_753 0.87 +0.2
863,680 - Pf6N2E2_753 0.87 -0.0
863,733 + -1.3
863,733 + -1.2
863,733 + -1.3
863,734 - +1.5
863,734 - +0.2
863,734 - +0.3
863,734 - +0.0
863,745 + -0.8
863,828 - -0.7
863,905 + +1.5
863,905 + -0.4
863,905 + +0.3
863,919 + +0.2
863,919 + +0.6
863,919 + +0.7
864,297 + Pf6N2E2_754 0.84 -0.3
864,297 + Pf6N2E2_754 0.84 -0.3
864,348 - +0.3
864,604 + Pf6N2E2_755 0.12 +0.5
864,688 + Pf6N2E2_755 0.17 -1.4
864,688 + Pf6N2E2_755 0.17 -0.6
864,688 + Pf6N2E2_755 0.17 -0.6
864,688 + Pf6N2E2_755 0.17 +0.2
864,689 - Pf6N2E2_755 0.17 +1.1
864,689 - Pf6N2E2_755 0.17 -0.1
864,689 - Pf6N2E2_755 0.17 -0.3
864,776 - Pf6N2E2_755 0.22 +0.0
864,776 - Pf6N2E2_755 0.22 +0.2
864,776 - Pf6N2E2_755 0.22 -0.5
864,776 - Pf6N2E2_755 0.22 +0.1
864,776 - Pf6N2E2_755 0.22 +0.6

Or see this region's nucleotide sequence