Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_632

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_631 and Pf6N2E2_632 are separated by 287 nucleotidesPf6N2E2_632 and Pf6N2E2_633 are separated by 49 nucleotidesPf6N2E2_633 and Pf6N2E2_634 are separated by 431 nucleotides Pf6N2E2_631 - 2-ketogluconate utilization repressor PtxS, at 718,430 to 719,455 _631 Pf6N2E2_632 - hypothetical protein, at 719,743 to 721,851 _632 Pf6N2E2_633 - FIG00954580: hypothetical protein, at 721,901 to 722,254 _633 Pf6N2E2_634 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form, at 722,686 to 727,554 _634 Position (kb) 719 720 721 722Strain fitness (log2 ratio) -1 0 1 2 3at 719.083 kb on - strand, within Pf6N2E2_631at 719.083 kb on - strand, within Pf6N2E2_631at 719.083 kb on - strand, within Pf6N2E2_631at 719.152 kb on + strand, within Pf6N2E2_631at 719.152 kb on + strand, within Pf6N2E2_631at 719.153 kb on - strand, within Pf6N2E2_631at 719.153 kb on - strand, within Pf6N2E2_631at 719.153 kb on - strand, within Pf6N2E2_631at 719.472 kb on - strandat 719.472 kb on - strandat 719.481 kb on - strandat 719.481 kb on - strandat 719.481 kb on - strandat 719.484 kb on - strandat 719.736 kb on - strandat 719.773 kb on + strandat 719.773 kb on + strandat 719.774 kb on - strandat 719.774 kb on - strandat 719.776 kb on + strandat 719.776 kb on + strandat 719.777 kb on - strandat 719.805 kb on - strandat 719.897 kb on - strandat 720.008 kb on - strand, within Pf6N2E2_632at 720.187 kb on + strand, within Pf6N2E2_632at 720.188 kb on - strand, within Pf6N2E2_632at 720.377 kb on + strand, within Pf6N2E2_632at 720.471 kb on + strand, within Pf6N2E2_632at 720.471 kb on + strand, within Pf6N2E2_632at 720.472 kb on - strand, within Pf6N2E2_632at 720.472 kb on - strand, within Pf6N2E2_632at 720.472 kb on - strand, within Pf6N2E2_632at 720.472 kb on - strand, within Pf6N2E2_632at 720.543 kb on + strand, within Pf6N2E2_632at 720.543 kb on + strand, within Pf6N2E2_632at 720.544 kb on - strand, within Pf6N2E2_632at 720.593 kb on - strand, within Pf6N2E2_632at 720.595 kb on + strand, within Pf6N2E2_632at 720.595 kb on + strand, within Pf6N2E2_632at 720.595 kb on + strand, within Pf6N2E2_632at 720.596 kb on - strand, within Pf6N2E2_632at 720.596 kb on - strand, within Pf6N2E2_632at 720.754 kb on - strand, within Pf6N2E2_632at 720.838 kb on + strand, within Pf6N2E2_632at 720.839 kb on - strand, within Pf6N2E2_632at 720.869 kb on - strand, within Pf6N2E2_632at 720.930 kb on + strand, within Pf6N2E2_632at 720.945 kb on + strand, within Pf6N2E2_632at 720.946 kb on - strand, within Pf6N2E2_632at 720.949 kb on + strand, within Pf6N2E2_632at 720.949 kb on + strand, within Pf6N2E2_632at 720.950 kb on - strand, within Pf6N2E2_632at 720.950 kb on - strand, within Pf6N2E2_632at 720.950 kb on - strand, within Pf6N2E2_632at 720.950 kb on - strand, within Pf6N2E2_632at 720.950 kb on - strand, within Pf6N2E2_632at 720.950 kb on - strand, within Pf6N2E2_632at 720.950 kb on - strand, within Pf6N2E2_632at 720.950 kb on - strand, within Pf6N2E2_632at 721.022 kb on - strand, within Pf6N2E2_632at 721.050 kb on - strand, within Pf6N2E2_632at 721.050 kb on - strand, within Pf6N2E2_632at 721.066 kb on + strand, within Pf6N2E2_632at 721.067 kb on - strand, within Pf6N2E2_632at 721.135 kb on + strand, within Pf6N2E2_632at 721.135 kb on + strand, within Pf6N2E2_632at 721.136 kb on - strand, within Pf6N2E2_632at 721.136 kb on - strand, within Pf6N2E2_632at 721.258 kb on + strand, within Pf6N2E2_632at 721.259 kb on - strand, within Pf6N2E2_632at 721.259 kb on - strand, within Pf6N2E2_632at 721.261 kb on - strand, within Pf6N2E2_632at 721.328 kb on - strand, within Pf6N2E2_632at 721.377 kb on + strand, within Pf6N2E2_632at 721.452 kb on + strand, within Pf6N2E2_632at 721.452 kb on + strand, within Pf6N2E2_632at 721.473 kb on + strand, within Pf6N2E2_632at 721.615 kb on + strand, within Pf6N2E2_632at 721.645 kb on + strandat 721.730 kb on - strandat 721.772 kb on - strandat 721.775 kb on - strandat 721.913 kb on + strandat 721.913 kb on + strandat 721.913 kb on + strandat 721.913 kb on + strandat 721.913 kb on + strandat 721.914 kb on - strandat 721.914 kb on - strandat 721.914 kb on - strandat 721.915 kb on + strandat 721.915 kb on + strandat 721.915 kb on + strandat 721.915 kb on + strandat 721.915 kb on + strandat 721.915 kb on + strandat 721.915 kb on + strandat 721.915 kb on + strandat 721.915 kb on + strandat 721.916 kb on - strandat 721.916 kb on - strandat 721.916 kb on - strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.917 kb on + strandat 721.918 kb on - strandat 721.918 kb on - strandat 721.918 kb on - strandat 721.918 kb on - strandat 721.918 kb on - strandat 721.918 kb on - strandat 722.415 kb on + strandat 722.415 kb on + strandat 722.416 kb on - strandat 722.529 kb on + strandat 722.530 kb on - strandat 722.530 kb on - strandat 722.530 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
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719,083 - Pf6N2E2_631 0.64 +1.2
719,083 - Pf6N2E2_631 0.64 +0.0
719,083 - Pf6N2E2_631 0.64 +1.0
719,152 + Pf6N2E2_631 0.70 +1.0
719,152 + Pf6N2E2_631 0.70 -0.5
719,153 - Pf6N2E2_631 0.70 +0.4
719,153 - Pf6N2E2_631 0.70 +0.5
719,153 - Pf6N2E2_631 0.70 +1.2
719,472 - -0.5
719,472 - +0.1
719,481 - +0.2
719,481 - +1.5
719,481 - -0.9
719,484 - -0.3
719,736 - +1.5
719,773 + -0.1
719,773 + -1.1
719,774 - +2.5
719,774 - -0.1
719,776 + -0.2
719,776 + +1.8
719,777 - -0.5
719,805 - +1.0
719,897 - -0.6
720,008 - Pf6N2E2_632 0.13 +3.1
720,187 + Pf6N2E2_632 0.21 -0.2
720,188 - Pf6N2E2_632 0.21 +0.2
720,377 + Pf6N2E2_632 0.30 +0.1
720,471 + Pf6N2E2_632 0.35 +0.3
720,471 + Pf6N2E2_632 0.35 +0.0
720,472 - Pf6N2E2_632 0.35 -0.4
720,472 - Pf6N2E2_632 0.35 +0.4
720,472 - Pf6N2E2_632 0.35 +0.2
720,472 - Pf6N2E2_632 0.35 +0.3
720,543 + Pf6N2E2_632 0.38 -0.2
720,543 + Pf6N2E2_632 0.38 +0.6
720,544 - Pf6N2E2_632 0.38 +0.7
720,593 - Pf6N2E2_632 0.40 +0.3
720,595 + Pf6N2E2_632 0.40 +0.7
720,595 + Pf6N2E2_632 0.40 +1.7
720,595 + Pf6N2E2_632 0.40 -1.0
720,596 - Pf6N2E2_632 0.40 -0.2
720,596 - Pf6N2E2_632 0.40 -0.4
720,754 - Pf6N2E2_632 0.48 -1.0
720,838 + Pf6N2E2_632 0.52 +0.8
720,839 - Pf6N2E2_632 0.52 -0.2
720,869 - Pf6N2E2_632 0.53 -1.1
720,930 + Pf6N2E2_632 0.56 +0.2
720,945 + Pf6N2E2_632 0.57 +0.3
720,946 - Pf6N2E2_632 0.57 +1.5
720,949 + Pf6N2E2_632 0.57 +0.3
720,949 + Pf6N2E2_632 0.57 -0.2
720,950 - Pf6N2E2_632 0.57 -0.2
720,950 - Pf6N2E2_632 0.57 -0.2
720,950 - Pf6N2E2_632 0.57 +0.0
720,950 - Pf6N2E2_632 0.57 -0.3
720,950 - Pf6N2E2_632 0.57 +0.8
720,950 - Pf6N2E2_632 0.57 +0.7
720,950 - Pf6N2E2_632 0.57 -0.3
720,950 - Pf6N2E2_632 0.57 -0.4
721,022 - Pf6N2E2_632 0.61 -0.7
721,050 - Pf6N2E2_632 0.62 -0.5
721,050 - Pf6N2E2_632 0.62 -0.2
721,066 + Pf6N2E2_632 0.63 +0.4
721,067 - Pf6N2E2_632 0.63 +0.7
721,135 + Pf6N2E2_632 0.66 +0.5
721,135 + Pf6N2E2_632 0.66 +2.5
721,136 - Pf6N2E2_632 0.66 +0.1
721,136 - Pf6N2E2_632 0.66 +0.6
721,258 + Pf6N2E2_632 0.72 +1.5
721,259 - Pf6N2E2_632 0.72 +1.1
721,259 - Pf6N2E2_632 0.72 -0.8
721,261 - Pf6N2E2_632 0.72 +0.6
721,328 - Pf6N2E2_632 0.75 -0.3
721,377 + Pf6N2E2_632 0.77 +0.8
721,452 + Pf6N2E2_632 0.81 +0.3
721,452 + Pf6N2E2_632 0.81 +0.4
721,473 + Pf6N2E2_632 0.82 +0.5
721,615 + Pf6N2E2_632 0.89 +0.5
721,645 + +0.4
721,730 - -0.4
721,772 - +1.5
721,775 - -0.4
721,913 + -0.4
721,913 + +0.3
721,913 + -1.1
721,913 + -0.3
721,913 + +1.1
721,914 - +1.5
721,914 - +1.5
721,914 - +0.8
721,915 + +0.1
721,915 + +0.1
721,915 + -0.5
721,915 + +0.0
721,915 + -0.4
721,915 + +0.5
721,915 + +2.1
721,915 + -0.5
721,915 + -1.6
721,916 - +0.2
721,916 - -0.9
721,916 - +0.3
721,917 + -0.4
721,917 + +1.5
721,917 + +0.3
721,917 + +0.4
721,917 + +0.2
721,917 + +1.7
721,917 + -0.5
721,917 + -0.6
721,917 + -0.5
721,917 + -0.1
721,917 + +1.5
721,918 - +0.5
721,918 - -0.2
721,918 - -1.4
721,918 - +0.3
721,918 - +0.6
721,918 - +1.5
722,415 + -0.7
722,415 + -0.9
722,416 - +1.5
722,529 + +0.5
722,530 - -0.5
722,530 - -0.5
722,530 - +0.7

Or see this region's nucleotide sequence