Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_563 and Pf6N2E2_564 are separated by 312 nucleotides Pf6N2E2_564 and Pf6N2E2_565 are separated by 27 nucleotides Pf6N2E2_565 and Pf6N2E2_566 are separated by 165 nucleotides
Pf6N2E2_563 - Serine transporter, at 636,957 to 638,237
_563
Pf6N2E2_564 - Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 638,550 to 641,159
_564
Pf6N2E2_565 - hypothetical protein, at 641,187 to 641,402
_565
Pf6N2E2_566 - hypothetical protein, at 641,568 to 642,038
_566
Position (kb) 638
639
640
641
642 Strain fitness (log2 ratio) -2
-1
0
1 at 637.586 kb on + strand, within Pf6N2E2_563 at 637.586 kb on + strand, within Pf6N2E2_563 at 637.587 kb on - strand, within Pf6N2E2_563 at 637.587 kb on - strand, within Pf6N2E2_563 at 637.830 kb on + strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 638.121 kb on + strand at 638.122 kb on - strand at 638.122 kb on - strand at 638.221 kb on - strand at 638.345 kb on + strand at 638.346 kb on - strand at 638.445 kb on + strand at 638.494 kb on + strand at 638.494 kb on + strand at 638.495 kb on - strand at 638.495 kb on - strand at 638.545 kb on - strand at 638.545 kb on - strand at 641.422 kb on - strand at 641.422 kb on - strand at 641.440 kb on - strand at 641.440 kb on - strand at 641.480 kb on - strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.998 kb on - strand at 642.001 kb on + strand at 642.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 637,586 + Pf6N2E2_563 0.49 +1.4 637,586 + Pf6N2E2_563 0.49 +0.7 637,587 - Pf6N2E2_563 0.49 -0.8 637,587 - Pf6N2E2_563 0.49 +1.4 637,830 + Pf6N2E2_563 0.68 +0.1 637,921 - Pf6N2E2_563 0.75 -0.3 637,921 - Pf6N2E2_563 0.75 -1.1 637,921 - Pf6N2E2_563 0.75 -0.2 638,121 + -0.6 638,122 - -0.4 638,122 - -0.7 638,221 - -2.0 638,345 + +0.0 638,346 - +0.2 638,445 + -0.9 638,494 + +0.0 638,494 + +1.2 638,495 - -0.6 638,495 - -1.1 638,545 - -0.9 638,545 - -0.1 641,422 - -0.6 641,422 - +0.9 641,440 - +0.9 641,440 - -1.9 641,480 - -0.1 641,997 + +0.7 641,997 + -1.1 641,997 + +0.3 641,997 + +1.4 641,998 - -1.1 642,001 + +1.1 642,002 - +0.1
Or see this region's nucleotide sequence