Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5384

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
6,186,518	-		Pf6N2E2_5383	0.43	+0.8
6,186,518	-		Pf6N2E2_5383	0.43	-0.4
6,186,518	-		Pf6N2E2_5383	0.43	+0.1
6,186,518	-		Pf6N2E2_5383	0.43	+1.4
6,186,553	+		Pf6N2E2_5383	0.46	+0.0
6,186,553	+		Pf6N2E2_5383	0.46	+0.5
6,186,554	-		Pf6N2E2_5383	0.46	-0.6
6,186,559	+				+0.2
6,186,559	+		Pf6N2E2_5383	0.46	-0.1
6,186,560	-		Pf6N2E2_5383	0.46	-0.9
6,186,649	+		Pf6N2E2_5383	0.52	+0.1
6,186,650	-		Pf6N2E2_5383	0.52	+1.4
6,186,659	+		Pf6N2E2_5383	0.52	-0.5
6,186,659	+		Pf6N2E2_5383	0.52	+0.2
6,186,660	-		Pf6N2E2_5383	0.52	+0.3
6,186,660	-		Pf6N2E2_5383	0.52	+0.7
6,186,677	+		Pf6N2E2_5383	0.53	-0.6
6,186,678	-		Pf6N2E2_5383	0.53	+0.8
6,186,678	-		Pf6N2E2_5383	0.53	-0.1
6,186,678	-		Pf6N2E2_5383	0.53	-0.7
6,186,678	-		Pf6N2E2_5383	0.53	+1.4
6,186,678	-		Pf6N2E2_5383	0.53	-0.1
6,186,721	+		Pf6N2E2_5383	0.56	-0.0
6,186,721	+		Pf6N2E2_5383	0.56	-0.6
6,186,721	+		Pf6N2E2_5383	0.56	-0.6
6,186,721	+		Pf6N2E2_5383	0.56	-0.3
6,186,721	+		Pf6N2E2_5383	0.56	-0.1
6,186,722	-		Pf6N2E2_5383	0.56	-0.5
6,186,722	-		Pf6N2E2_5383	0.56	-0.1
6,186,955	+		Pf6N2E2_5383	0.71	-0.0
6,186,955	+		Pf6N2E2_5383	0.71	-0.5
6,186,982	+		Pf6N2E2_5383	0.73	+0.1
6,186,982	+		Pf6N2E2_5383	0.73	-0.1
6,186,982	+		Pf6N2E2_5383	0.73	-0.6
6,186,983	-		Pf6N2E2_5383	0.73	+0.1
6,187,021	+		Pf6N2E2_5383	0.75	+0.2
6,187,021	+		Pf6N2E2_5383	0.75	+0.5
6,187,022	-		Pf6N2E2_5383	0.75	+1.2
6,187,195	+		Pf6N2E2_5383	0.86	-0.3
6,187,196	-		Pf6N2E2_5383	0.86	-0.4
6,187,264	+				+0.6
6,187,264	+				+0.1
6,187,264	+				+2.4
6,187,264	+				-0.7
6,187,264	+				-0.5
6,187,264	+				+0.5
6,187,265	-				+0.3
6,187,265	-				+0.1
6,187,265	-				-0.6
6,187,265	-				-0.3
6,187,409	-				-1.0
6,187,409	-				-0.7
6,187,485	+				-0.0
6,187,486	-				-0.3
6,187,486	-				+1.1
6,187,506	+				-1.8
6,187,609	-		Pf6N2E2_5384	0.11	+0.1
6,187,609	-		Pf6N2E2_5384	0.11	+1.1
6,187,779	+		Pf6N2E2_5384	0.22	+0.3
6,187,779	+		Pf6N2E2_5384	0.22	+0.4
6,187,779	+		Pf6N2E2_5384	0.22	-0.9
6,187,779	+		Pf6N2E2_5384	0.22	-0.2
6,187,779	+		Pf6N2E2_5384	0.22	-0.4
6,187,779	+		Pf6N2E2_5384	0.22	+0.1
6,187,780	-		Pf6N2E2_5384	0.22	-1.9
6,187,941	+		Pf6N2E2_5384	0.33	+0.2
6,187,962	+		Pf6N2E2_5384	0.34	-0.6
6,188,130	+		Pf6N2E2_5384	0.46	+0.3
6,188,131	-		Pf6N2E2_5384	0.46	+0.1
6,188,160	+		Pf6N2E2_5384	0.48	+0.3
6,188,160	+		Pf6N2E2_5384	0.48	-0.3
6,188,160	+		Pf6N2E2_5384	0.48	-0.9
6,188,160	+		Pf6N2E2_5384	0.48	+0.6
6,188,251	-		Pf6N2E2_5384	0.54	-0.2
6,188,698	-		Pf6N2E2_5384	0.84	-0.3
6,188,730	+		Pf6N2E2_5384	0.86	-0.6
6,188,731	-		Pf6N2E2_5384	0.86	-1.1
6,188,731	-		Pf6N2E2_5384	0.86	+1.4
6,188,944	+				+0.9
6,188,971	+				-1.0
6,189,031	+				+0.1
6,189,031	+				+0.0
6,189,031	+				+0.3
6,189,068	-				-1.2
6,189,143	+		Pf6N2E2_5385	0.14	-1.0
6,189,143	+		Pf6N2E2_5385	0.14	+0.1
6,189,144	-		Pf6N2E2_5385	0.14	+0.4
6,189,144	-		Pf6N2E2_5385	0.14	+0.3
6,189,144	-		Pf6N2E2_5385	0.14	+0.0
6,189,144	-		Pf6N2E2_5385	0.14	+1.6
6,189,144	-		Pf6N2E2_5385	0.14	-0.2
6,189,144	-		Pf6N2E2_5385	0.14	-0.3
6,189,144	-		Pf6N2E2_5385	0.14	+0.6
6,189,144	-		Pf6N2E2_5385	0.14	+0.8
6,189,266	+		Pf6N2E2_5385	0.23	+0.5
6,189,293	+		Pf6N2E2_5385	0.25	+1.4
6,189,293	+		Pf6N2E2_5385	0.25	-0.6
6,189,293	+		Pf6N2E2_5385	0.25	-0.7
6,189,293	+		Pf6N2E2_5385	0.25	+1.0
6,189,294	-		Pf6N2E2_5385	0.25	+0.0
6,189,308	+		Pf6N2E2_5385	0.26	-0.6
6,189,309	-		Pf6N2E2_5385	0.26	+1.4
6,189,309	-		Pf6N2E2_5385	0.26	+2.0
6,189,311	+		Pf6N2E2_5385	0.26	+0.4
6,189,395	+		Pf6N2E2_5385	0.32	-1.2
6,189,396	-		Pf6N2E2_5385	0.32	+1.1
6,189,396	-		Pf6N2E2_5385	0.32	+1.4
6,189,396	-		Pf6N2E2_5385	0.32	+0.3
6,189,396	-		Pf6N2E2_5385	0.32	-1.0
6,189,398	+		Pf6N2E2_5385	0.33	-1.2
6,189,399	-		Pf6N2E2_5385	0.33	-0.4
6,189,458	+		Pf6N2E2_5385	0.37	-0.2
6,189,458	+		Pf6N2E2_5385	0.37	+0.6
6,189,459	-		Pf6N2E2_5385	0.37	+0.2
6,189,501	-		Pf6N2E2_5385	0.40	+0.2
6,189,719	+		Pf6N2E2_5385	0.56	+0.1
6,189,720	-		Pf6N2E2_5385	0.56	-0.3
6,189,740	+		Pf6N2E2_5385	0.57	-0.6
6,189,740	+		Pf6N2E2_5385	0.57	-0.1
6,189,740	+		Pf6N2E2_5385	0.57	-0.7
6,189,741	-		Pf6N2E2_5385	0.57	-0.2
6,189,806	+		Pf6N2E2_5385	0.62	+0.6
6,189,806	+		Pf6N2E2_5385	0.62	-0.4
6,189,807	-		Pf6N2E2_5385	0.62	+0.0
6,189,807	-		Pf6N2E2_5385	0.62	-0.5
6,189,807	-		Pf6N2E2_5385	0.62	-1.0
6,189,807	-		Pf6N2E2_5385	0.62	-1.4
6,189,929	+		Pf6N2E2_5385	0.71	+0.3
6,189,929	+		Pf6N2E2_5385	0.71	+0.4
6,189,929	+		Pf6N2E2_5385	0.71	-0.2
6,189,930	-		Pf6N2E2_5385	0.71	+0.4
6,189,930	-		Pf6N2E2_5385	0.71	-1.4