Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4656

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4655 and Pf6N2E2_4656 are separated by 148 nucleotidesPf6N2E2_4656 and Pf6N2E2_4657 are separated by 24 nucleotides Pf6N2E2_4655 - Transketolase (EC 2.2.1.1), at 5,384,424 to 5,386,421 _4655 Pf6N2E2_4656 - D-erythrose-4-phosphate dehydrogenase, at 5,386,570 to 5,387,625 _4656 Pf6N2E2_4657 - Phosphoglycerate kinase (EC 2.7.2.3), at 5,387,650 to 5,388,813 _4657 Position (kb) 5386 5387 5388Strain fitness (log2 ratio) -1 0 1at 5386.588 kb on + strandat 5386.789 kb on - strand, within Pf6N2E2_4656at 5387.623 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
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5,386,588 + +0.3
5,386,789 - Pf6N2E2_4656 0.21 -0.3
5,387,623 + -0.5

Or see this region's nucleotide sequence