Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4431

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
5,161,390	+				+1.2
5,161,390	+				-0.1
5,161,428	+				-1.1
5,161,428	+				-1.3
5,161,428	+				+1.0
5,161,470	+				-0.9
5,161,471	-				-1.6
5,161,471	-				-0.9
5,161,546	+		Pf6N2E2_4430	0.10	+0.6
5,161,546	+		Pf6N2E2_4430	0.10	+0.1
5,161,547	-		Pf6N2E2_4430	0.10	-1.1
5,161,618	-		Pf6N2E2_4430	0.20	-0.3
5,161,658	+		Pf6N2E2_4430	0.25	-0.7
5,161,658	+		Pf6N2E2_4430	0.25	-0.7
5,161,658	+		Pf6N2E2_4430	0.25	-1.3
5,161,658	+		Pf6N2E2_4430	0.25	-1.4
5,161,659	-		Pf6N2E2_4430	0.25	-0.1
5,161,713	+		Pf6N2E2_4430	0.32	-0.6
5,161,714	-		Pf6N2E2_4430	0.33	-0.7
5,161,714	-		Pf6N2E2_4430	0.33	-1.2
5,161,765	+		Pf6N2E2_4430	0.39	-1.2
5,161,893	+		Pf6N2E2_4430	0.56	-0.3
5,161,894	-		Pf6N2E2_4430	0.57	+0.3
5,161,948	+		Pf6N2E2_4430	0.64	-0.2
5,161,948	+		Pf6N2E2_4430	0.64	+0.2
5,161,948	+		Pf6N2E2_4430	0.64	-1.8
5,161,948	+		Pf6N2E2_4430	0.64	-0.6
5,161,948	+		Pf6N2E2_4430	0.64	-0.8
5,161,949	-		Pf6N2E2_4430	0.64	+0.0
5,161,949	-		Pf6N2E2_4430	0.64	-0.7
5,161,949	-		Pf6N2E2_4430	0.64	-1.0
5,161,969	+		Pf6N2E2_4430	0.67	-2.2
5,161,969	+		Pf6N2E2_4430	0.67	-1.1
5,161,969	+		Pf6N2E2_4430	0.67	-3.1
5,161,969	+		Pf6N2E2_4430	0.67	-0.3
5,161,969	+		Pf6N2E2_4430	0.67	+0.6
5,161,969	+		Pf6N2E2_4430	0.67	+0.1
5,161,969	+		Pf6N2E2_4430	0.67	-2.5
5,161,969	+		Pf6N2E2_4430	0.67	-2.2
5,161,969	+		Pf6N2E2_4430	0.67	-0.4
5,161,970	-		Pf6N2E2_4430	0.67	-0.6
5,161,970	-		Pf6N2E2_4430	0.67	-0.8
5,161,970	-		Pf6N2E2_4430	0.67	-0.2
5,161,970	-		Pf6N2E2_4430	0.67	-0.2
5,161,970	-		Pf6N2E2_4430	0.67	-1.1
5,161,970	-		Pf6N2E2_4430	0.67	-0.6
5,161,970	-		Pf6N2E2_4430	0.67	-0.6
5,162,215	+				-0.2
5,162,215	+				-0.1
5,162,215	+				+0.1
5,162,278	+				-0.6
5,162,319	+				-0.7
5,162,319	+				+1.0
5,162,331	+				-0.2
5,162,338	+				-0.9
5,162,342	+				+1.5
5,163,273	+				+2.5
5,164,057	+		Pf6N2E2_4432	0.40	-0.0
5,164,058	-		Pf6N2E2_4432	0.40	-0.2
5,164,073	+		Pf6N2E2_4432	0.41	+0.6
5,164,073	+		Pf6N2E2_4432	0.41	-0.1
5,164,073	+		Pf6N2E2_4432	0.41	+0.4
5,164,073	+		Pf6N2E2_4432	0.41	-0.0
5,164,073	+		Pf6N2E2_4432	0.41	-0.6
5,164,073	+		Pf6N2E2_4432	0.41	+0.0
5,164,074	-		Pf6N2E2_4432	0.41	+0.5
5,164,074	-		Pf6N2E2_4432	0.41	+0.2
5,164,107	+		Pf6N2E2_4432	0.42	-0.2
5,164,107	+		Pf6N2E2_4432	0.42	+0.1
5,164,107	+		Pf6N2E2_4432	0.42	+2.8
5,164,107	+		Pf6N2E2_4432	0.42	+0.2
5,164,108	-		Pf6N2E2_4432	0.43	+0.1
5,164,108	-		Pf6N2E2_4432	0.43	+0.4
5,164,108	-		Pf6N2E2_4432	0.43	-0.3
5,164,182	+		Pf6N2E2_4432	0.46	-0.6
5,164,182	+		Pf6N2E2_4432	0.46	-0.8
5,164,182	+		Pf6N2E2_4432	0.46	-1.6
5,164,182	+		Pf6N2E2_4432	0.46	-0.7
5,164,183	-		Pf6N2E2_4432	0.46	-1.5
5,164,183	-		Pf6N2E2_4432	0.46	-1.1
5,164,183	-		Pf6N2E2_4432	0.46	+1.2
5,164,183	-		Pf6N2E2_4432	0.46	-1.0
5,164,183	-		Pf6N2E2_4432	0.46	-0.4
5,164,183	-		Pf6N2E2_4432	0.46	-2.7
5,164,183	-		Pf6N2E2_4432	0.46	-3.0
5,164,262	-		Pf6N2E2_4432	0.51	+0.8