Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3954

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3953 and Pf6N2E2_3954 are separated by 280 nucleotidesPf6N2E2_3954 and Pf6N2E2_3955 are separated by 46 nucleotidesPf6N2E2_3955 and Pf6N2E2_3956 are separated by 209 nucleotides Pf6N2E2_3953 - Methionine ABC transporter substrate-binding protein, at 4,594,272 to 4,595,054 _3953 Pf6N2E2_3954 - Functional role page for Anaerobic nitric oxide reductase transcription regulator NorR, at 4,595,335 to 4,596,270 _3954 Pf6N2E2_3955 - FIG00953394: hypothetical protein, at 4,596,317 to 4,596,898 _3955 Pf6N2E2_3956 - FIG00960943: hypothetical protein, at 4,597,108 to 4,597,353 _3956 Position (kb) 4595 4596 4597Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4594.408 kb on + strand, within Pf6N2E2_3953at 4594.409 kb on - strand, within Pf6N2E2_3953at 4594.409 kb on - strand, within Pf6N2E2_3953at 4594.409 kb on - strand, within Pf6N2E2_3953at 4594.409 kb on - strand, within Pf6N2E2_3953at 4594.431 kb on + strand, within Pf6N2E2_3953at 4594.454 kb on + strand, within Pf6N2E2_3953at 4594.455 kb on - strand, within Pf6N2E2_3953at 4594.474 kb on + strand, within Pf6N2E2_3953at 4594.474 kb on + strand, within Pf6N2E2_3953at 4594.474 kb on + strand, within Pf6N2E2_3953at 4594.474 kb on + strand, within Pf6N2E2_3953at 4594.474 kb on + strand, within Pf6N2E2_3953at 4594.474 kb on + strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.475 kb on - strand, within Pf6N2E2_3953at 4594.575 kb on - strand, within Pf6N2E2_3953at 4594.720 kb on + strand, within Pf6N2E2_3953at 4594.810 kb on + strand, within Pf6N2E2_3953at 4594.811 kb on - strand, within Pf6N2E2_3953at 4594.828 kb on + strand, within Pf6N2E2_3953at 4594.828 kb on + strand, within Pf6N2E2_3953at 4594.828 kb on + strand, within Pf6N2E2_3953at 4594.829 kb on - strand, within Pf6N2E2_3953at 4594.829 kb on - strand, within Pf6N2E2_3953at 4594.829 kb on - strand, within Pf6N2E2_3953at 4594.829 kb on - strand, within Pf6N2E2_3953at 4594.829 kb on - strand, within Pf6N2E2_3953at 4594.829 kb on - strand, within Pf6N2E2_3953at 4594.829 kb on - strand, within Pf6N2E2_3953at 4594.860 kb on + strand, within Pf6N2E2_3953at 4594.860 kb on + strand, within Pf6N2E2_3953at 4594.860 kb on + strand, within Pf6N2E2_3953at 4594.860 kb on + strand, within Pf6N2E2_3953at 4594.860 kb on + strand, within Pf6N2E2_3953at 4594.861 kb on - strand, within Pf6N2E2_3953at 4594.861 kb on - strand, within Pf6N2E2_3953at 4594.877 kb on - strand, within Pf6N2E2_3953at 4595.208 kb on - strandat 4595.208 kb on - strandat 4595.336 kb on + strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.337 kb on - strandat 4595.621 kb on + strand, within Pf6N2E2_3954at 4595.621 kb on + strand, within Pf6N2E2_3954at 4595.621 kb on + strand, within Pf6N2E2_3954at 4595.621 kb on + strand, within Pf6N2E2_3954at 4595.621 kb on + strand, within Pf6N2E2_3954at 4595.622 kb on - strand, within Pf6N2E2_3954at 4595.622 kb on - strand, within Pf6N2E2_3954at 4595.708 kb on + strand, within Pf6N2E2_3954at 4595.708 kb on + strand, within Pf6N2E2_3954at 4595.708 kb on + strand, within Pf6N2E2_3954at 4595.708 kb on + strand, within Pf6N2E2_3954at 4595.709 kb on - strand, within Pf6N2E2_3954at 4595.709 kb on - strand, within Pf6N2E2_3954at 4595.709 kb on - strand, within Pf6N2E2_3954at 4595.709 kb on - strand, within Pf6N2E2_3954at 4595.714 kb on + strand, within Pf6N2E2_3954at 4595.791 kb on + strand, within Pf6N2E2_3954at 4595.792 kb on - strand, within Pf6N2E2_3954at 4595.958 kb on - strand, within Pf6N2E2_3954at 4595.958 kb on - strand, within Pf6N2E2_3954at 4595.958 kb on - strand, within Pf6N2E2_3954at 4595.997 kb on - strand, within Pf6N2E2_3954at 4596.063 kb on + strand, within Pf6N2E2_3954at 4596.063 kb on + strand, within Pf6N2E2_3954at 4596.063 kb on + strand, within Pf6N2E2_3954at 4596.064 kb on - strand, within Pf6N2E2_3954at 4596.064 kb on - strand, within Pf6N2E2_3954at 4596.064 kb on - strand, within Pf6N2E2_3954at 4596.364 kb on - strandat 4596.370 kb on - strandat 4596.370 kb on - strandat 4596.370 kb on - strandat 4596.415 kb on + strand, within Pf6N2E2_3955at 4596.415 kb on + strand, within Pf6N2E2_3955at 4596.415 kb on + strand, within Pf6N2E2_3955at 4596.416 kb on - strand, within Pf6N2E2_3955at 4596.416 kb on - strand, within Pf6N2E2_3955at 4596.416 kb on - strand, within Pf6N2E2_3955at 4596.563 kb on - strand, within Pf6N2E2_3955at 4596.691 kb on + strand, within Pf6N2E2_3955at 4596.691 kb on + strand, within Pf6N2E2_3955at 4596.876 kb on + strandat 4596.877 kb on - strandat 4596.932 kb on + strandat 4596.933 kb on - strandat 4596.934 kb on + strandat 4596.934 kb on + strandat 4596.934 kb on + strandat 4596.934 kb on + strandat 4596.934 kb on + strandat 4596.934 kb on + strandat 4596.935 kb on - strandat 4596.935 kb on - strandat 4596.935 kb on - strandat 4596.935 kb on - strandat 4596.935 kb on - strandat 4596.935 kb on - strandat 4596.948 kb on - strandat 4596.952 kb on + strandat 4597.046 kb on + strandat 4597.047 kb on - strandat 4597.047 kb on - strandat 4597.150 kb on + strand, within Pf6N2E2_3956at 4597.150 kb on + strand, within Pf6N2E2_3956at 4597.150 kb on + strand, within Pf6N2E2_3956

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
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4,594,408 + Pf6N2E2_3953 0.17 -0.9
4,594,409 - Pf6N2E2_3953 0.17 +1.2
4,594,409 - Pf6N2E2_3953 0.17 +0.1
4,594,409 - Pf6N2E2_3953 0.17 +0.2
4,594,409 - Pf6N2E2_3953 0.17 +0.4
4,594,431 + Pf6N2E2_3953 0.20 -0.6
4,594,454 + Pf6N2E2_3953 0.23 +0.1
4,594,455 - Pf6N2E2_3953 0.23 -0.2
4,594,474 + Pf6N2E2_3953 0.26 -0.3
4,594,474 + Pf6N2E2_3953 0.26 +0.5
4,594,474 + Pf6N2E2_3953 0.26 -0.7
4,594,474 + Pf6N2E2_3953 0.26 -0.7
4,594,474 + Pf6N2E2_3953 0.26 +0.0
4,594,474 + Pf6N2E2_3953 0.26 +0.1
4,594,475 - Pf6N2E2_3953 0.26 -0.3
4,594,475 - Pf6N2E2_3953 0.26 +0.7
4,594,475 - Pf6N2E2_3953 0.26 -2.2
4,594,475 - Pf6N2E2_3953 0.26 +1.4
4,594,475 - Pf6N2E2_3953 0.26 -0.3
4,594,475 - Pf6N2E2_3953 0.26 -0.9
4,594,475 - Pf6N2E2_3953 0.26 +0.5
4,594,475 - Pf6N2E2_3953 0.26 +0.2
4,594,475 - Pf6N2E2_3953 0.26 +0.5
4,594,475 - Pf6N2E2_3953 0.26 -0.2
4,594,475 - Pf6N2E2_3953 0.26 -0.3
4,594,475 - Pf6N2E2_3953 0.26 +0.4
4,594,475 - Pf6N2E2_3953 0.26 +0.0
4,594,575 - Pf6N2E2_3953 0.39 -0.2
4,594,720 + Pf6N2E2_3953 0.57 -0.6
4,594,810 + Pf6N2E2_3953 0.69 -0.8
4,594,811 - Pf6N2E2_3953 0.69 -0.7
4,594,828 + Pf6N2E2_3953 0.71 -0.6
4,594,828 + Pf6N2E2_3953 0.71 -0.6
4,594,828 + Pf6N2E2_3953 0.71 -1.2
4,594,829 - Pf6N2E2_3953 0.71 +0.4
4,594,829 - Pf6N2E2_3953 0.71 +1.2
4,594,829 - Pf6N2E2_3953 0.71 +0.2
4,594,829 - Pf6N2E2_3953 0.71 -0.2
4,594,829 - Pf6N2E2_3953 0.71 +0.5
4,594,829 - Pf6N2E2_3953 0.71 -0.3
4,594,829 - Pf6N2E2_3953 0.71 +0.1
4,594,860 + Pf6N2E2_3953 0.75 -1.4
4,594,860 + Pf6N2E2_3953 0.75 +0.3
4,594,860 + Pf6N2E2_3953 0.75 -0.6
4,594,860 + Pf6N2E2_3953 0.75 +0.0
4,594,860 + Pf6N2E2_3953 0.75 -0.7
4,594,861 - Pf6N2E2_3953 0.75 -0.5
4,594,861 - Pf6N2E2_3953 0.75 -0.4
4,594,877 - Pf6N2E2_3953 0.77 -0.5
4,595,208 - -0.2
4,595,208 - +0.5
4,595,336 + +1.0
4,595,337 - +2.8
4,595,337 - -0.5
4,595,337 - +0.8
4,595,337 - -1.0
4,595,337 - -0.5
4,595,337 - -1.3
4,595,337 - -0.5
4,595,337 - -1.2
4,595,337 - -1.0
4,595,337 - +1.0
4,595,337 - -0.0
4,595,621 + Pf6N2E2_3954 0.31 -0.0
4,595,621 + Pf6N2E2_3954 0.31 +0.1
4,595,621 + Pf6N2E2_3954 0.31 -0.4
4,595,621 + Pf6N2E2_3954 0.31 +0.4
4,595,621 + Pf6N2E2_3954 0.31 -0.6
4,595,622 - Pf6N2E2_3954 0.31 +0.2
4,595,622 - Pf6N2E2_3954 0.31 -0.1
4,595,708 + Pf6N2E2_3954 0.40 -0.6
4,595,708 + Pf6N2E2_3954 0.40 +0.0
4,595,708 + Pf6N2E2_3954 0.40 -0.0
4,595,708 + Pf6N2E2_3954 0.40 -0.3
4,595,709 - Pf6N2E2_3954 0.40 +1.8
4,595,709 - Pf6N2E2_3954 0.40 -0.8
4,595,709 - Pf6N2E2_3954 0.40 +0.1
4,595,709 - Pf6N2E2_3954 0.40 +1.2
4,595,714 + Pf6N2E2_3954 0.40 +1.2
4,595,791 + Pf6N2E2_3954 0.49 +0.8
4,595,792 - Pf6N2E2_3954 0.49 +0.1
4,595,958 - Pf6N2E2_3954 0.67 -0.1
4,595,958 - Pf6N2E2_3954 0.67 +0.1
4,595,958 - Pf6N2E2_3954 0.67 -0.1
4,595,997 - Pf6N2E2_3954 0.71 -0.8
4,596,063 + Pf6N2E2_3954 0.78 +0.4
4,596,063 + Pf6N2E2_3954 0.78 -0.4
4,596,063 + Pf6N2E2_3954 0.78 +0.0
4,596,064 - Pf6N2E2_3954 0.78 +0.5
4,596,064 - Pf6N2E2_3954 0.78 -1.2
4,596,064 - Pf6N2E2_3954 0.78 -0.4
4,596,364 - +2.2
4,596,370 - -1.1
4,596,370 - +2.2
4,596,370 - -1.2
4,596,415 + Pf6N2E2_3955 0.17 +0.4
4,596,415 + Pf6N2E2_3955 0.17 +0.5
4,596,415 + Pf6N2E2_3955 0.17 -0.1
4,596,416 - Pf6N2E2_3955 0.17 -0.8
4,596,416 - Pf6N2E2_3955 0.17 -0.6
4,596,416 - Pf6N2E2_3955 0.17 +0.2
4,596,563 - Pf6N2E2_3955 0.42 -1.6
4,596,691 + Pf6N2E2_3955 0.64 -0.1
4,596,691 + Pf6N2E2_3955 0.64 -0.5
4,596,876 + +0.4
4,596,877 - -0.5
4,596,932 + +0.4
4,596,933 - -0.3
4,596,934 + +0.9
4,596,934 + -0.2
4,596,934 + -0.6
4,596,934 + -0.1
4,596,934 + -1.1
4,596,934 + +0.1
4,596,935 - -0.1
4,596,935 - -0.2
4,596,935 - -1.2
4,596,935 - -0.4
4,596,935 - -0.1
4,596,935 - -0.4
4,596,948 - -0.5
4,596,952 + -0.5
4,597,046 + -0.2
4,597,047 - -0.1
4,597,047 - +0.5
4,597,150 + Pf6N2E2_3956 0.17 -0.1
4,597,150 + Pf6N2E2_3956 0.17 -1.4
4,597,150 + Pf6N2E2_3956 0.17 +0.0

Or see this region's nucleotide sequence