Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3813

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
4,441,784	+		Pf6N2E2_3811	0.89	+0.1
4,441,784	+		Pf6N2E2_3811	0.89	+1.2
4,441,784	+		Pf6N2E2_3811	0.89	+0.2
4,441,785	-		Pf6N2E2_3811	0.89	-0.8
4,441,979	+				-0.6
4,441,980	-				-0.4
4,441,992	+				-0.1
4,441,992	+				+0.1
4,441,992	+				-0.1
4,441,993	-				-1.0
4,442,036	+				+0.0
4,442,095	+				-0.2
4,442,352	+		Pf6N2E2_3812	0.41	-0.3
4,442,352	+		Pf6N2E2_3812	0.41	-0.0
4,442,353	-		Pf6N2E2_3812	0.41	-0.2
4,442,360	+		Pf6N2E2_3812	0.42	-0.4
4,442,360	+		Pf6N2E2_3812	0.42	+0.2
4,442,360	+		Pf6N2E2_3812	0.42	+0.8
4,442,360	+		Pf6N2E2_3812	0.42	-0.3
4,442,360	+		Pf6N2E2_3812	0.42	-1.1
4,442,361	-		Pf6N2E2_3812	0.42	-0.7
4,442,361	-		Pf6N2E2_3812	0.42	+0.4
4,442,709	+				+0.1
4,442,710	-				-0.4
4,442,710	-				-0.3
4,442,710	-				-0.2
4,442,710	-				+0.1
4,443,779	-				+0.1
4,443,779	-				+0.9
4,443,789	-				+0.5
4,443,789	-				-0.2
4,443,789	-				-1.5
4,443,789	-				-0.7
4,443,918	+				+0.5
4,443,918	+				+0.2
4,444,011	+				+0.3
4,444,012	-				+0.0
4,444,012	-				-0.9
4,444,012	-				+0.2
4,444,012	-				-0.3
4,444,131	-		Pf6N2E2_3814	0.13	+0.2
4,444,159	+		Pf6N2E2_3814	0.15	+0.1
4,444,552	+		Pf6N2E2_3814	0.43	+1.4
4,444,552	+		Pf6N2E2_3814	0.43	-0.2
4,444,552	+		Pf6N2E2_3814	0.43	+0.6
4,444,552	+		Pf6N2E2_3814	0.43	-0.0
4,444,552	+		Pf6N2E2_3814	0.43	+0.2
4,444,553	-		Pf6N2E2_3814	0.44	+0.6
4,444,553	-		Pf6N2E2_3814	0.44	+0.5
4,444,553	-		Pf6N2E2_3814	0.44	+0.4
4,444,553	-		Pf6N2E2_3814	0.44	+0.2
4,444,553	-		Pf6N2E2_3814	0.44	+0.7
4,444,553	-		Pf6N2E2_3814	0.44	+0.0
4,444,694	-		Pf6N2E2_3814	0.54	-0.3