Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_334 and Pf6N2E2_335 are separated by 100 nucleotides Pf6N2E2_335 and Pf6N2E2_336 overlap by 4 nucleotides Pf6N2E2_336 and Pf6N2E2_337 are separated by 173 nucleotides
Pf6N2E2_334 - Transcriptional regulator, at 401,717 to 402,670
_334
Pf6N2E2_335 - Aldo-keto reductase, at 402,771 to 403,634
_335
Pf6N2E2_336 - hypothetical protein, at 403,631 to 404,413
_336
Pf6N2E2_337 - hypothetical protein, at 404,587 to 404,859
_337
Position (kb) 402
403
404 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 401.838 kb on + strand, within Pf6N2E2_334 at 401.841 kb on + strand, within Pf6N2E2_334 at 401.910 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.938 kb on - strand, within Pf6N2E2_334 at 401.957 kb on + strand, within Pf6N2E2_334 at 401.958 kb on - strand, within Pf6N2E2_334 at 402.051 kb on + strand, within Pf6N2E2_334 at 402.051 kb on + strand, within Pf6N2E2_334 at 402.150 kb on + strand, within Pf6N2E2_334 at 402.150 kb on + strand, within Pf6N2E2_334 at 402.151 kb on - strand, within Pf6N2E2_334 at 402.211 kb on + strand, within Pf6N2E2_334 at 402.212 kb on - strand, within Pf6N2E2_334 at 402.337 kb on + strand, within Pf6N2E2_334 at 402.686 kb on - strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.957 kb on + strand, within Pf6N2E2_335 at 403.006 kb on - strand, within Pf6N2E2_335 at 403.332 kb on + strand, within Pf6N2E2_335 at 403.332 kb on + strand, within Pf6N2E2_335 at 403.395 kb on + strand, within Pf6N2E2_335 at 403.395 kb on + strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.633 kb on - strand at 403.865 kb on + strand, within Pf6N2E2_336 at 403.865 kb on + strand, within Pf6N2E2_336 at 403.865 kb on + strand, within Pf6N2E2_336 at 403.865 kb on + strand, within Pf6N2E2_336 at 403.866 kb on - strand, within Pf6N2E2_336 at 403.866 kb on - strand at 403.866 kb on - strand, within Pf6N2E2_336 at 403.938 kb on + strand, within Pf6N2E2_336 at 403.938 kb on + strand, within Pf6N2E2_336 at 403.938 kb on + strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.116 kb on - strand, within Pf6N2E2_336 at 404.467 kb on + strand at 404.467 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 401,838 + Pf6N2E2_334 0.13 +1.5 401,841 + Pf6N2E2_334 0.13 +0.3 401,910 + Pf6N2E2_334 0.20 -0.4 401,937 + Pf6N2E2_334 0.23 +0.9 401,937 + Pf6N2E2_334 0.23 +0.0 401,937 + Pf6N2E2_334 0.23 -1.9 401,938 - Pf6N2E2_334 0.23 +0.6 401,957 + Pf6N2E2_334 0.25 -1.7 401,958 - Pf6N2E2_334 0.25 -1.5 402,051 + Pf6N2E2_334 0.35 -0.4 402,051 + Pf6N2E2_334 0.35 -0.4 402,150 + Pf6N2E2_334 0.45 +0.1 402,150 + Pf6N2E2_334 0.45 +0.5 402,151 - Pf6N2E2_334 0.45 +0.4 402,211 + Pf6N2E2_334 0.52 -0.8 402,212 - Pf6N2E2_334 0.52 +0.9 402,337 + Pf6N2E2_334 0.65 -0.8 402,686 - -0.0 402,733 + -0.1 402,733 + -2.9 402,733 + +1.5 402,733 + -1.1 402,733 + -0.1 402,734 - -0.9 402,734 - -1.5 402,734 - -1.5 402,734 - +0.5 402,734 - +0.5 402,734 - +0.3 402,957 + Pf6N2E2_335 0.22 +0.6 403,006 - Pf6N2E2_335 0.27 +0.4 403,332 + Pf6N2E2_335 0.65 +1.5 403,332 + Pf6N2E2_335 0.65 +1.2 403,395 + Pf6N2E2_335 0.72 -0.6 403,395 + Pf6N2E2_335 0.72 -1.6 403,396 - Pf6N2E2_335 0.72 +0.4 403,396 - Pf6N2E2_335 0.72 -0.6 403,396 - Pf6N2E2_335 0.72 -0.5 403,633 - -1.5 403,865 + Pf6N2E2_336 0.30 +0.2 403,865 + Pf6N2E2_336 0.30 -0.4 403,865 + Pf6N2E2_336 0.30 +0.5 403,865 + Pf6N2E2_336 0.30 -0.7 403,866 - Pf6N2E2_336 0.30 +0.7 403,866 - -0.9 403,866 - Pf6N2E2_336 0.30 +0.9 403,938 + Pf6N2E2_336 0.39 +0.2 403,938 + Pf6N2E2_336 0.39 -0.7 403,938 + Pf6N2E2_336 0.39 -0.2 404,039 - Pf6N2E2_336 0.52 +0.5 404,039 - Pf6N2E2_336 0.52 -0.7 404,039 - Pf6N2E2_336 0.52 -1.4 404,116 - Pf6N2E2_336 0.62 +1.5 404,467 + +0.5 404,467 + +1.5 404,536 + +0.1 404,536 + +0.6 404,536 + +0.3 404,537 - +0.3
Or see this region's nucleotide sequence