Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2993 and Pf6N2E2_2994 are separated by 83 nucleotides Pf6N2E2_2994 and Pf6N2E2_2995 are separated by 71 nucleotides Pf6N2E2_2995 and Pf6N2E2_2996 overlap by 19 nucleotides
Pf6N2E2_2993 - RecA protein, at 3,484,974 to 3,486,026
_2993
Pf6N2E2_2994 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 3,486,110 to 3,486,610
_2994
Pf6N2E2_2995 - FIG00959768: hypothetical protein, at 3,486,682 to 3,487,218
_2995
Pf6N2E2_2996 - FIG101079: Lytic enzyme, at 3,487,200 to 3,487,763
_2996
Position (kb)
3486
3487 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3485.466 kb on + strand, within Pf6N2E2_2993 at 3485.816 kb on + strand, within Pf6N2E2_2993 at 3485.953 kb on - strand at 3486.041 kb on - strand at 3486.041 kb on - strand at 3486.041 kb on - strand at 3486.041 kb on - strand at 3486.041 kb on - strand at 3486.131 kb on + strand at 3486.131 kb on + strand at 3486.302 kb on + strand, within Pf6N2E2_2994 at 3486.303 kb on - strand, within Pf6N2E2_2994 at 3486.340 kb on + strand, within Pf6N2E2_2994 at 3486.340 kb on + strand, within Pf6N2E2_2994 at 3486.450 kb on + strand, within Pf6N2E2_2994 at 3486.450 kb on + strand, within Pf6N2E2_2994 at 3486.450 kb on + strand, within Pf6N2E2_2994 at 3486.450 kb on + strand, within Pf6N2E2_2994 at 3486.450 kb on + strand, within Pf6N2E2_2994 at 3486.450 kb on + strand, within Pf6N2E2_2994 at 3486.451 kb on - strand, within Pf6N2E2_2994 at 3486.451 kb on - strand, within Pf6N2E2_2994 at 3486.459 kb on + strand, within Pf6N2E2_2994 at 3486.460 kb on - strand, within Pf6N2E2_2994 at 3486.460 kb on - strand, within Pf6N2E2_2994 at 3486.460 kb on - strand, within Pf6N2E2_2994 at 3486.460 kb on - strand, within Pf6N2E2_2994 at 3486.460 kb on - strand, within Pf6N2E2_2994 at 3486.643 kb on - strand at 3486.647 kb on + strand at 3486.647 kb on + strand at 3486.648 kb on - strand at 3486.648 kb on - strand at 3486.702 kb on + strand at 3486.702 kb on + strand at 3486.702 kb on + strand at 3486.702 kb on + strand at 3486.704 kb on + strand at 3486.704 kb on + strand at 3486.704 kb on + strand at 3486.704 kb on + strand at 3486.704 kb on + strand at 3486.705 kb on - strand at 3486.705 kb on - strand at 3486.705 kb on - strand at 3486.705 kb on - strand at 3486.705 kb on - strand at 3486.705 kb on - strand at 3486.903 kb on + strand, within Pf6N2E2_2995 at 3486.903 kb on + strand, within Pf6N2E2_2995 at 3486.904 kb on - strand, within Pf6N2E2_2995 at 3486.904 kb on - strand, within Pf6N2E2_2995 at 3486.933 kb on + strand, within Pf6N2E2_2995 at 3486.933 kb on + strand, within Pf6N2E2_2995 at 3486.933 kb on + strand, within Pf6N2E2_2995 at 3486.934 kb on - strand, within Pf6N2E2_2995 at 3487.219 kb on - strand at 3487.280 kb on + strand, within Pf6N2E2_2996 at 3487.436 kb on + strand, within Pf6N2E2_2996 at 3487.436 kb on + strand, within Pf6N2E2_2996 at 3487.436 kb on + strand, within Pf6N2E2_2996 at 3487.436 kb on + strand, within Pf6N2E2_2996 at 3487.437 kb on - strand, within Pf6N2E2_2996 at 3487.437 kb on - strand, within Pf6N2E2_2996 at 3487.447 kb on + strand, within Pf6N2E2_2996 at 3487.448 kb on - strand, within Pf6N2E2_2996 at 3487.448 kb on - strand, within Pf6N2E2_2996 at 3487.448 kb on - strand, within Pf6N2E2_2996 at 3487.490 kb on - strand, within Pf6N2E2_2996 at 3487.555 kb on + strand, within Pf6N2E2_2996 at 3487.555 kb on + strand, within Pf6N2E2_2996 at 3487.555 kb on + strand, within Pf6N2E2_2996 at 3487.555 kb on + strand, within Pf6N2E2_2996 at 3487.556 kb on - strand, within Pf6N2E2_2996 at 3487.556 kb on - strand, within Pf6N2E2_2996 at 3487.556 kb on - strand, within Pf6N2E2_2996 at 3487.567 kb on + strand, within Pf6N2E2_2996 at 3487.567 kb on + strand, within Pf6N2E2_2996 at 3487.594 kb on + strand, within Pf6N2E2_2996 at 3487.595 kb on - strand, within Pf6N2E2_2996
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A remove 3,485,466 + Pf6N2E2_2993 0.47 +1.4 3,485,816 + Pf6N2E2_2993 0.80 +1.4 3,485,953 - +1.4 3,486,041 - -0.3 3,486,041 - +0.4 3,486,041 - -1.1 3,486,041 - -1.3 3,486,041 - +1.1 3,486,131 + +2.4 3,486,131 + -1.0 3,486,302 + Pf6N2E2_2994 0.38 +1.4 3,486,303 - Pf6N2E2_2994 0.39 -1.3 3,486,340 + Pf6N2E2_2994 0.46 -0.6 3,486,340 + Pf6N2E2_2994 0.46 +0.4 3,486,450 + Pf6N2E2_2994 0.68 -1.4 3,486,450 + Pf6N2E2_2994 0.68 -1.2 3,486,450 + Pf6N2E2_2994 0.68 -1.0 3,486,450 + Pf6N2E2_2994 0.68 -1.3 3,486,450 + Pf6N2E2_2994 0.68 -0.2 3,486,450 + Pf6N2E2_2994 0.68 -0.1 3,486,451 - Pf6N2E2_2994 0.68 -2.7 3,486,451 - Pf6N2E2_2994 0.68 -1.6 3,486,459 + Pf6N2E2_2994 0.70 -1.2 3,486,460 - Pf6N2E2_2994 0.70 -1.6 3,486,460 - Pf6N2E2_2994 0.70 +0.4 3,486,460 - Pf6N2E2_2994 0.70 -1.6 3,486,460 - Pf6N2E2_2994 0.70 -2.9 3,486,460 - Pf6N2E2_2994 0.70 +0.4 3,486,643 - +1.4 3,486,647 + +0.1 3,486,647 + -1.2 3,486,648 - -0.7 3,486,648 - +0.2 3,486,702 + -2.3 3,486,702 + -0.4 3,486,702 + +1.2 3,486,702 + -0.2 3,486,704 + -0.4 3,486,704 + -1.0 3,486,704 + -1.6 3,486,704 + -0.5 3,486,704 + -0.2 3,486,705 - +1.7 3,486,705 - -0.1 3,486,705 - -1.6 3,486,705 - -0.7 3,486,705 - -0.6 3,486,705 - +0.4 3,486,903 + Pf6N2E2_2995 0.41 +0.7 3,486,903 + Pf6N2E2_2995 0.41 -0.6 3,486,904 - Pf6N2E2_2995 0.41 +0.5 3,486,904 - Pf6N2E2_2995 0.41 +0.3 3,486,933 + Pf6N2E2_2995 0.47 -0.4 3,486,933 + Pf6N2E2_2995 0.47 +0.1 3,486,933 + Pf6N2E2_2995 0.47 +0.2 3,486,934 - Pf6N2E2_2995 0.47 +1.3 3,487,219 - +2.4 3,487,280 + Pf6N2E2_2996 0.14 -0.5 3,487,436 + Pf6N2E2_2996 0.42 +0.6 3,487,436 + Pf6N2E2_2996 0.42 +2.0 3,487,436 + Pf6N2E2_2996 0.42 -0.1 3,487,436 + Pf6N2E2_2996 0.42 -1.2 3,487,437 - Pf6N2E2_2996 0.42 -0.0 3,487,437 - Pf6N2E2_2996 0.42 +0.9 3,487,447 + Pf6N2E2_2996 0.44 -0.4 3,487,448 - Pf6N2E2_2996 0.44 -0.2 3,487,448 - Pf6N2E2_2996 0.44 -1.2 3,487,448 - Pf6N2E2_2996 0.44 +0.5 3,487,490 - Pf6N2E2_2996 0.51 -1.6 3,487,555 + Pf6N2E2_2996 0.63 -0.3 3,487,555 + Pf6N2E2_2996 0.63 -2.3 3,487,555 + Pf6N2E2_2996 0.63 +0.2 3,487,555 + Pf6N2E2_2996 0.63 -0.8 3,487,556 - Pf6N2E2_2996 0.63 -0.0 3,487,556 - Pf6N2E2_2996 0.63 -0.7 3,487,556 - Pf6N2E2_2996 0.63 -1.4 3,487,567 + Pf6N2E2_2996 0.65 +0.4 3,487,567 + Pf6N2E2_2996 0.65 -0.2 3,487,594 + Pf6N2E2_2996 0.70 +0.5 3,487,595 - Pf6N2E2_2996 0.70 +0.0
Or see this region's nucleotide sequence