Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1547

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
1,805,504	-				-0.3
1,805,515	+				-0.4
1,805,516	-				+0.3
1,805,955	+		Pf6N2E2_1546	0.53	-0.2
1,805,956	-		Pf6N2E2_1546	0.53	+0.3
1,806,049	+		Pf6N2E2_1546	0.64	-0.9
1,806,050	-		Pf6N2E2_1546	0.64	+0.8
1,806,071	+		Pf6N2E2_1546	0.67	+0.1
1,806,071	+		Pf6N2E2_1546	0.67	+0.9
1,806,072	-		Pf6N2E2_1546	0.67	-0.6
1,806,072	-		Pf6N2E2_1546	0.67	+1.5
1,806,072	-		Pf6N2E2_1546	0.67	+0.3
1,806,072	-		Pf6N2E2_1546	0.67	-1.3
1,806,072	-		Pf6N2E2_1546	0.67	-0.7
1,806,239	+		Pf6N2E2_1546	0.86	-0.4
1,806,240	-		Pf6N2E2_1546	0.86	-1.7
1,806,240	-		Pf6N2E2_1546	0.86	-1.1
1,806,323	+				-1.0
1,806,324	-				+1.5
1,806,346	-				-1.8
1,806,375	+				+1.1
1,806,376	-				+0.5
1,806,386	+				-0.4
1,806,387	-				-0.8
1,806,401	+				-0.5
1,806,402	-				+0.2
1,806,406	+				+2.1
1,806,419	+				-1.0
1,806,570	-		Pf6N2E2_1547	0.15	+0.1
1,806,771	+		Pf6N2E2_1547	0.36	-1.1
1,806,772	-		Pf6N2E2_1547	0.36	-0.5
1,806,858	+		Pf6N2E2_1547	0.45	+0.0
1,806,858	+		Pf6N2E2_1547	0.45	-0.5
1,806,859	-		Pf6N2E2_1547	0.45	-0.9
1,806,989	+		Pf6N2E2_1547	0.58	-1.3
1,806,990	-		Pf6N2E2_1547	0.58	-0.1
1,806,990	-		Pf6N2E2_1547	0.58	-0.4
1,806,990	-		Pf6N2E2_1547	0.58	-0.9
1,807,223	+		Pf6N2E2_1547	0.83	-0.3
1,807,223	+		Pf6N2E2_1547	0.83	-0.5
1,807,224	-		Pf6N2E2_1547	0.83	-0.4
1,807,414	-				+0.9
1,807,414	-				+0.5
1,807,414	-				+0.2
1,807,414	-				+0.2
1,807,414	-				+0.0
1,807,427	+				-0.5
1,807,498	+				-1.0
1,807,499	-				-1.0
1,807,499	-				-0.8
1,807,732	-		Pf6N2E2_1548	0.62	+0.5
1,807,804	-		Pf6N2E2_1548	0.81	+0.1
1,807,804	-		Pf6N2E2_1548	0.81	-0.3
1,807,806	+		Pf6N2E2_1548	0.81	-0.1
1,807,815	+		Pf6N2E2_1548	0.84	-1.4
1,807,816	-		Pf6N2E2_1548	0.84	+0.5
1,807,875	+				+0.1
1,807,875	+				-0.8
1,807,961	+				+2.5
1,807,962	-				-0.3
1,808,097	-		Pf6N2E2_1549	0.21	+1.5
1,808,244	+		Pf6N2E2_1549	0.37	-0.9
1,808,307	+		Pf6N2E2_1549	0.44	-1.2
1,808,308	-		Pf6N2E2_1549	0.44	-0.6