Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_812 and Pf6N2E2_813 are separated by 77 nucleotides Pf6N2E2_813 and Pf6N2E2_814 are separated by 212 nucleotides Pf6N2E2_814 and Pf6N2E2_815 overlap by 30 nucleotides
Pf6N2E2_812 - Glycosyltransferase, at 944,886 to 946,178
_812
Pf6N2E2_813 - Response regulator, at 946,256 to 946,561
_813
Pf6N2E2_814 - hypothetical protein, at 946,774 to 946,917
_814
Pf6N2E2_815 - Signal transduction histidine kinase, at 946,888 to 950,370
_815
Position (kb) 946
947 Strain fitness (log2 ratio) -2
-1
0
1
2 at 945.369 kb on - strand, within Pf6N2E2_812 at 945.397 kb on + strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.407 kb on - strand, within Pf6N2E2_812 at 946.138 kb on + strand at 946.138 kb on + strand at 946.138 kb on + strand at 946.138 kb on + strand at 946.139 kb on - strand at 946.342 kb on + strand, within Pf6N2E2_813 at 946.343 kb on - strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.627 kb on + strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.690 kb on + strand at 946.690 kb on + strand at 946.690 kb on + strand at 946.691 kb on - strand at 946.724 kb on - strand at 946.737 kb on + strand at 946.737 kb on + strand at 946.738 kb on - strand at 946.739 kb on + strand at 946.740 kb on - strand at 946.742 kb on + strand at 946.775 kb on + strand at 946.776 kb on - strand at 946.782 kb on + strand at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.944 kb on + strand at 946.944 kb on + strand at 946.944 kb on + strand at 947.005 kb on + strand at 947.005 kb on + strand at 947.005 kb on + strand at 947.006 kb on - strand at 947.146 kb on + strand at 947.184 kb on + strand at 947.185 kb on - strand at 947.185 kb on - strand at 947.240 kb on + strand, within Pf6N2E2_815 at 947.240 kb on + strand, within Pf6N2E2_815 at 947.240 kb on + strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.270 kb on - strand, within Pf6N2E2_815 at 947.270 kb on - strand, within Pf6N2E2_815 at 947.458 kb on + strand, within Pf6N2E2_815 at 947.458 kb on + strand, within Pf6N2E2_815 at 947.459 kb on - strand, within Pf6N2E2_815 at 947.497 kb on + strand, within Pf6N2E2_815 at 947.498 kb on - strand, within Pf6N2E2_815 at 947.498 kb on - strand, within Pf6N2E2_815 at 947.536 kb on + strand, within Pf6N2E2_815 at 947.536 kb on + strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 945,369 - Pf6N2E2_812 0.37 +1.0 945,397 + Pf6N2E2_812 0.40 -0.1 945,398 - Pf6N2E2_812 0.40 -0.4 945,398 - Pf6N2E2_812 0.40 +1.3 945,398 - Pf6N2E2_812 0.40 +0.6 945,407 - Pf6N2E2_812 0.40 -0.1 946,138 + -1.2 946,138 + +1.6 946,138 + +1.1 946,138 + -0.4 946,139 - +1.4 946,342 + Pf6N2E2_813 0.28 -0.4 946,343 - Pf6N2E2_813 0.28 +0.6 946,527 + Pf6N2E2_813 0.89 -0.7 946,527 + Pf6N2E2_813 0.89 +0.6 946,527 + Pf6N2E2_813 0.89 +1.6 946,527 + Pf6N2E2_813 0.89 +1.6 946,527 + Pf6N2E2_813 0.89 -2.3 946,627 + +1.6 946,628 - +2.6 946,628 - +1.6 946,628 - +1.6 946,628 - +1.6 946,628 - +0.6 946,628 - +1.6 946,628 - +1.1 946,690 + +1.6 946,690 + +1.6 946,690 + -0.6 946,691 - +0.6 946,724 - +1.6 946,737 + -0.4 946,737 + +1.6 946,738 - +1.6 946,739 + +0.6 946,740 - -0.4 946,742 + +1.6 946,775 + +1.6 946,776 - +0.1 946,782 + +1.6 946,819 + Pf6N2E2_814 0.31 +1.6 946,819 + Pf6N2E2_814 0.31 +1.6 946,819 + Pf6N2E2_814 0.31 -2.4 946,819 + Pf6N2E2_814 0.31 +0.8 946,820 - Pf6N2E2_814 0.32 -0.9 946,820 - Pf6N2E2_814 0.32 -0.1 946,820 - Pf6N2E2_814 0.32 +0.4 946,820 - Pf6N2E2_814 0.32 +0.5 946,944 + +0.1 946,944 + +0.2 946,944 + +1.6 947,005 + -1.7 947,005 + +1.6 947,005 + +1.6 947,006 - +1.6 947,146 + -2.3 947,184 + -2.3 947,185 - +1.6 947,185 - +0.6 947,240 + Pf6N2E2_815 0.10 +1.6 947,240 + Pf6N2E2_815 0.10 +2.4 947,240 + Pf6N2E2_815 0.10 +1.6 947,241 - Pf6N2E2_815 0.10 +0.4 947,241 - Pf6N2E2_815 0.10 +1.0 947,241 - Pf6N2E2_815 0.10 -1.1 947,241 - Pf6N2E2_815 0.10 +1.6 947,270 - Pf6N2E2_815 0.11 +1.6 947,270 - Pf6N2E2_815 0.11 +1.6 947,458 + Pf6N2E2_815 0.16 +1.6 947,458 + Pf6N2E2_815 0.16 +0.6 947,459 - Pf6N2E2_815 0.16 +1.6 947,497 + Pf6N2E2_815 0.17 +1.6 947,498 - Pf6N2E2_815 0.18 +1.6 947,498 - Pf6N2E2_815 0.18 +1.6 947,536 + Pf6N2E2_815 0.19 +0.1 947,536 + Pf6N2E2_815 0.19 +0.1 947,537 - Pf6N2E2_815 0.19 +0.2 947,537 - Pf6N2E2_815 0.19 +1.6 947,537 - Pf6N2E2_815 0.19 -0.0 947,537 - Pf6N2E2_815 0.19 +1.6
Or see this region's nucleotide sequence