Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotides Pf6N2E2_65 and Pf6N2E2_66 are separated by 66 nucleotides Pf6N2E2_66 and Pf6N2E2_67 are separated by 54 nucleotides
Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084
_64
Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987
_65
Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136
_66
Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303
_67
Position (kb)
106
107
108 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 105.092 kb on + strand at 105.251 kb on + strand at 105.252 kb on - strand at 105.252 kb on - strand at 105.283 kb on + strand at 105.329 kb on + strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.437 kb on - strand, within Pf6N2E2_65 at 105.697 kb on + strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.801 kb on + strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.855 kb on + strand, within Pf6N2E2_65 at 105.954 kb on + strand at 105.955 kb on - strand at 106.013 kb on - strand at 106.013 kb on - strand at 106.147 kb on + strand at 106.147 kb on + strand at 106.148 kb on - strand at 106.148 kb on - strand at 106.470 kb on + strand, within Pf6N2E2_66 at 106.470 kb on + strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.543 kb on + strand, within Pf6N2E2_66 at 106.637 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.975 kb on + strand, within Pf6N2E2_66 at 107.076 kb on + strand at 107.111 kb on + strand at 107.177 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 105,092 + -0.9 105,251 + +0.4 105,252 - -0.3 105,252 - -1.6 105,283 + +1.0 105,329 + Pf6N2E2_65 0.14 -0.2 105,330 - Pf6N2E2_65 0.14 +0.2 105,330 - Pf6N2E2_65 0.14 -0.9 105,330 - Pf6N2E2_65 0.14 -1.1 105,330 - Pf6N2E2_65 0.14 +0.5 105,330 - Pf6N2E2_65 0.14 +0.0 105,436 + Pf6N2E2_65 0.28 -0.3 105,436 + Pf6N2E2_65 0.28 +0.3 105,437 - Pf6N2E2_65 0.28 +0.1 105,697 + Pf6N2E2_65 0.62 -0.2 105,698 - Pf6N2E2_65 0.62 +0.5 105,698 - Pf6N2E2_65 0.62 -0.4 105,801 + Pf6N2E2_65 0.76 +0.9 105,802 - Pf6N2E2_65 0.76 -2.6 105,802 - Pf6N2E2_65 0.76 +0.6 105,829 + Pf6N2E2_65 0.79 -0.9 105,829 + Pf6N2E2_65 0.79 -0.3 105,855 + Pf6N2E2_65 0.83 +0.2 105,954 + +0.1 105,955 - -1.4 106,013 - -1.2 106,013 - -0.7 106,147 + -2.8 106,147 + -0.9 106,148 - -2.2 106,148 - -2.6 106,470 + Pf6N2E2_66 0.38 -3.4 106,470 + Pf6N2E2_66 0.38 -3.3 106,475 - Pf6N2E2_66 0.39 +1.6 106,475 - Pf6N2E2_66 0.39 -3.0 106,475 - Pf6N2E2_66 0.39 +1.6 106,529 - Pf6N2E2_66 0.44 +1.6 106,529 - Pf6N2E2_66 0.44 +0.0 106,543 + Pf6N2E2_66 0.45 -4.0 106,637 + Pf6N2E2_66 0.54 -2.2 106,717 + Pf6N2E2_66 0.61 -3.0 106,717 + Pf6N2E2_66 0.61 -2.4 106,717 + Pf6N2E2_66 0.61 -2.9 106,717 + Pf6N2E2_66 0.61 -2.4 106,717 + Pf6N2E2_66 0.61 -3.0 106,717 + Pf6N2E2_66 0.61 -2.7 106,718 - Pf6N2E2_66 0.61 -3.8 106,718 - Pf6N2E2_66 0.61 -4.0 106,718 - Pf6N2E2_66 0.61 +0.6 106,718 - Pf6N2E2_66 0.61 -3.2 106,718 - Pf6N2E2_66 0.61 -1.9 106,718 - Pf6N2E2_66 0.61 +1.6 106,718 - Pf6N2E2_66 0.61 +1.6 106,882 + Pf6N2E2_66 0.76 -4.4 106,882 + Pf6N2E2_66 0.76 -4.2 106,883 - Pf6N2E2_66 0.77 +1.6 106,883 - Pf6N2E2_66 0.77 -1.7 106,883 - Pf6N2E2_66 0.77 -1.7 106,883 - Pf6N2E2_66 0.77 -1.1 106,883 - Pf6N2E2_66 0.77 -3.1 106,975 + Pf6N2E2_66 0.85 -3.2 107,076 + -2.3 107,111 + +0.5 107,177 + -2.2
Or see this region's nucleotide sequence