Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5921 and Pf6N2E2_5922 are separated by 90 nucleotides Pf6N2E2_5922 and Pf6N2E2_5923 are separated by 101 nucleotides Pf6N2E2_5923 and Pf6N2E2_5924 are separated by 232 nucleotides
Pf6N2E2_5921 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 6,740,569 to 6,741,195
_5921
Pf6N2E2_5922 - Transcriptional regulator, LysR family, at 6,741,286 to 6,742,284
_5922
Pf6N2E2_5923 - FIG00956241: hypothetical protein, at 6,742,386 to 6,742,793
_5923
Pf6N2E2_5924 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 6,743,026 to 6,745,473
_5924
Position (kb)
6741
6742
6743 Strain fitness (log2 ratio)
-2
-1
0
1 at 6740.381 kb on + strand at 6740.382 kb on - strand at 6740.426 kb on + strand at 6740.582 kb on + strand at 6740.583 kb on - strand at 6740.583 kb on - strand at 6740.596 kb on + strand at 6740.596 kb on + strand at 6740.597 kb on - strand at 6740.597 kb on - strand at 6740.597 kb on - strand at 6740.679 kb on - strand, within Pf6N2E2_5921 at 6740.825 kb on - strand, within Pf6N2E2_5921 at 6740.825 kb on - strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.991 kb on - strand, within Pf6N2E2_5921 at 6740.991 kb on - strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.202 kb on + strand at 6741.229 kb on + strand at 6741.230 kb on - strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.300 kb on - strand at 6741.300 kb on - strand at 6741.411 kb on + strand, within Pf6N2E2_5922 at 6741.411 kb on + strand, within Pf6N2E2_5922 at 6741.597 kb on - strand, within Pf6N2E2_5922 at 6741.814 kb on + strand, within Pf6N2E2_5922 at 6741.815 kb on - strand, within Pf6N2E2_5922 at 6741.815 kb on - strand, within Pf6N2E2_5922 at 6741.831 kb on + strand, within Pf6N2E2_5922 at 6741.831 kb on + strand, within Pf6N2E2_5922 at 6741.832 kb on - strand, within Pf6N2E2_5922 at 6741.874 kb on + strand, within Pf6N2E2_5922 at 6741.875 kb on - strand, within Pf6N2E2_5922 at 6741.875 kb on - strand, within Pf6N2E2_5922 at 6742.025 kb on - strand, within Pf6N2E2_5922 at 6742.071 kb on - strand, within Pf6N2E2_5922 at 6742.071 kb on - strand, within Pf6N2E2_5922 at 6742.138 kb on + strand, within Pf6N2E2_5922 at 6742.333 kb on + strand at 6742.333 kb on + strand at 6742.333 kb on + strand at 6742.336 kb on - strand at 6742.336 kb on - strand at 6742.336 kb on - strand at 6742.522 kb on + strand, within Pf6N2E2_5923 at 6742.522 kb on + strand, within Pf6N2E2_5923 at 6742.522 kb on + strand, within Pf6N2E2_5923 at 6742.522 kb on + strand, within Pf6N2E2_5923 at 6742.522 kb on + strand, within Pf6N2E2_5923 at 6742.522 kb on + strand, within Pf6N2E2_5923 at 6742.523 kb on - strand, within Pf6N2E2_5923 at 6742.523 kb on - strand, within Pf6N2E2_5923 at 6742.523 kb on - strand, within Pf6N2E2_5923 at 6742.546 kb on + strand, within Pf6N2E2_5923 at 6742.547 kb on - strand, within Pf6N2E2_5923 at 6742.547 kb on - strand, within Pf6N2E2_5923 at 6742.627 kb on + strand, within Pf6N2E2_5923 at 6742.798 kb on + strand at 6742.798 kb on + strand at 6742.798 kb on + strand at 6742.798 kb on + strand at 6742.798 kb on + strand at 6742.799 kb on - strand at 6742.799 kb on - strand at 6742.829 kb on + strand at 6742.829 kb on + strand at 6742.829 kb on + strand at 6742.830 kb on - strand at 6742.830 kb on - strand at 6742.830 kb on - strand at 6742.830 kb on - strand at 6742.831 kb on + strand at 6742.831 kb on + strand at 6742.831 kb on + strand at 6742.832 kb on - strand at 6742.832 kb on - strand at 6742.855 kb on - strand at 6742.989 kb on + strand at 6742.989 kb on + strand at 6742.989 kb on + strand at 6742.990 kb on - strand at 6742.990 kb on - strand at 6743.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 6,740,381 + -0.1 6,740,382 - -0.3 6,740,426 + +0.2 6,740,582 + +0.6 6,740,583 - -0.1 6,740,583 - -0.6 6,740,596 + -0.0 6,740,596 + -0.8 6,740,597 - -0.1 6,740,597 - +1.6 6,740,597 - -1.2 6,740,679 - Pf6N2E2_5921 0.18 +0.1 6,740,825 - Pf6N2E2_5921 0.41 +0.3 6,740,825 - Pf6N2E2_5921 0.41 +0.9 6,740,990 + Pf6N2E2_5921 0.67 -0.6 6,740,990 + Pf6N2E2_5921 0.67 -0.5 6,740,990 + Pf6N2E2_5921 0.67 -0.0 6,740,991 - Pf6N2E2_5921 0.67 +0.3 6,740,991 - Pf6N2E2_5921 0.67 +0.6 6,741,025 + Pf6N2E2_5921 0.73 -0.4 6,741,025 + Pf6N2E2_5921 0.73 -0.5 6,741,025 + Pf6N2E2_5921 0.73 +1.0 6,741,202 + -0.0 6,741,229 + -1.0 6,741,230 - -0.1 6,741,231 + -0.3 6,741,231 + +0.6 6,741,231 + -0.0 6,741,231 + -0.0 6,741,231 + -0.2 6,741,231 + +0.1 6,741,231 + +1.6 6,741,231 + -1.1 6,741,231 + -1.4 6,741,231 + +1.0 6,741,231 + -1.1 6,741,232 - -1.0 6,741,232 - -0.6 6,741,232 - -0.4 6,741,232 - -0.1 6,741,232 - -0.7 6,741,232 - +1.6 6,741,232 - -0.6 6,741,300 - -0.1 6,741,300 - -0.7 6,741,411 + Pf6N2E2_5922 0.13 -0.9 6,741,411 + Pf6N2E2_5922 0.13 +0.4 6,741,597 - Pf6N2E2_5922 0.31 +1.6 6,741,814 + Pf6N2E2_5922 0.53 -0.1 6,741,815 - Pf6N2E2_5922 0.53 -0.4 6,741,815 - Pf6N2E2_5922 0.53 -0.4 6,741,831 + Pf6N2E2_5922 0.55 -2.7 6,741,831 + Pf6N2E2_5922 0.55 +0.2 6,741,832 - Pf6N2E2_5922 0.55 -0.6 6,741,874 + Pf6N2E2_5922 0.59 +0.2 6,741,875 - Pf6N2E2_5922 0.59 -1.4 6,741,875 - Pf6N2E2_5922 0.59 -1.2 6,742,025 - Pf6N2E2_5922 0.74 -0.4 6,742,071 - Pf6N2E2_5922 0.79 +0.6 6,742,071 - Pf6N2E2_5922 0.79 -0.2 6,742,138 + Pf6N2E2_5922 0.85 -1.1 6,742,333 + -1.7 6,742,333 + +1.3 6,742,333 + -1.1 6,742,336 - -1.0 6,742,336 - -1.0 6,742,336 - -0.6 6,742,522 + Pf6N2E2_5923 0.33 -1.4 6,742,522 + Pf6N2E2_5923 0.33 +0.5 6,742,522 + Pf6N2E2_5923 0.33 -0.4 6,742,522 + Pf6N2E2_5923 0.33 +0.2 6,742,522 + Pf6N2E2_5923 0.33 -1.4 6,742,522 + Pf6N2E2_5923 0.33 -0.5 6,742,523 - Pf6N2E2_5923 0.34 +0.6 6,742,523 - Pf6N2E2_5923 0.34 +0.2 6,742,523 - Pf6N2E2_5923 0.34 -0.5 6,742,546 + Pf6N2E2_5923 0.39 +0.2 6,742,547 - Pf6N2E2_5923 0.39 -0.2 6,742,547 - Pf6N2E2_5923 0.39 +1.6 6,742,627 + Pf6N2E2_5923 0.59 -1.6 6,742,798 + +0.3 6,742,798 + -0.1 6,742,798 + +0.2 6,742,798 + -0.1 6,742,798 + +0.6 6,742,799 - -0.3 6,742,799 - +0.5 6,742,829 + -1.2 6,742,829 + -0.1 6,742,829 + -0.1 6,742,830 - -0.6 6,742,830 - -0.0 6,742,830 - +0.6 6,742,830 - +0.1 6,742,831 + +0.0 6,742,831 + +0.1 6,742,831 + -2.3 6,742,832 - -1.0 6,742,832 - +0.4 6,742,855 - +0.2 6,742,989 + -2.1 6,742,989 + -0.5 6,742,989 + +0.8 6,742,990 - +1.0 6,742,990 - +0.5 6,743,051 - -0.3
Or see this region's nucleotide sequence