Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5819

Experiment: Nitrate reduction, replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5817 and Pf6N2E2_5818 are separated by 130 nucleotidesPf6N2E2_5818 and Pf6N2E2_5819 are separated by 254 nucleotidesPf6N2E2_5819 and Pf6N2E2_5820 are separated by 183 nucleotides Pf6N2E2_5817 - FIG00956876: hypothetical protein, at 6,635,512 to 6,635,832 _5817 Pf6N2E2_5818 - Isochorismatase (EC 3.3.2.1), at 6,635,963 to 6,636,553 _5818 Pf6N2E2_5819 - Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p, at 6,636,808 to 6,637,716 _5819 Pf6N2E2_5820 - FIG00957914: hypothetical protein, at 6,637,900 to 6,638,688 _5820 Position (kb) 6636 6637 6638Strain fitness (log2 ratio) -1 0 1at 6636.063 kb on + strand, within Pf6N2E2_5818at 6636.648 kb on + strandat 6636.648 kb on + strandat 6636.680 kb on + strandat 6636.681 kb on - strandat 6636.681 kb on - strandat 6636.701 kb on - strandat 6636.772 kb on + strandat 6636.974 kb on - strand, within Pf6N2E2_5819at 6637.060 kb on + strand, within Pf6N2E2_5819at 6637.060 kb on + strand, within Pf6N2E2_5819at 6637.240 kb on + strand, within Pf6N2E2_5819at 6637.240 kb on + strand, within Pf6N2E2_5819at 6637.277 kb on + strand, within Pf6N2E2_5819at 6637.278 kb on - strand, within Pf6N2E2_5819at 6637.414 kb on + strand, within Pf6N2E2_5819at 6637.414 kb on + strand, within Pf6N2E2_5819at 6637.415 kb on - strand, within Pf6N2E2_5819at 6637.415 kb on - strand, within Pf6N2E2_5819at 6637.415 kb on - strand, within Pf6N2E2_5819at 6637.454 kb on - strand, within Pf6N2E2_5819at 6637.556 kb on + strand, within Pf6N2E2_5819at 6637.556 kb on + strand, within Pf6N2E2_5819at 6637.557 kb on - strand, within Pf6N2E2_5819at 6638.189 kb on + strand, within Pf6N2E2_5820at 6638.190 kb on - strand, within Pf6N2E2_5820at 6638.190 kb on - strand, within Pf6N2E2_5820at 6638.320 kb on - strand, within Pf6N2E2_5820at 6638.516 kb on + strand, within Pf6N2E2_5820at 6638.688 kb on + strandat 6638.688 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C
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6,636,063 + Pf6N2E2_5818 0.17 -0.3
6,636,648 + -1.1
6,636,648 + -0.7
6,636,680 + -0.0
6,636,681 - -0.0
6,636,681 - -0.7
6,636,701 - -1.0
6,636,772 + +1.6
6,636,974 - Pf6N2E2_5819 0.18 +1.0
6,637,060 + Pf6N2E2_5819 0.28 -0.7
6,637,060 + Pf6N2E2_5819 0.28 +0.6
6,637,240 + Pf6N2E2_5819 0.48 +0.4
6,637,240 + Pf6N2E2_5819 0.48 +0.3
6,637,277 + Pf6N2E2_5819 0.52 +0.6
6,637,278 - Pf6N2E2_5819 0.52 -0.9
6,637,414 + Pf6N2E2_5819 0.67 +0.6
6,637,414 + Pf6N2E2_5819 0.67 -0.9
6,637,415 - Pf6N2E2_5819 0.67 -1.4
6,637,415 - Pf6N2E2_5819 0.67 +0.3
6,637,415 - Pf6N2E2_5819 0.67 -0.2
6,637,454 - Pf6N2E2_5819 0.71 -1.2
6,637,556 + Pf6N2E2_5819 0.82 +0.1
6,637,556 + Pf6N2E2_5819 0.82 +0.3
6,637,557 - Pf6N2E2_5819 0.82 -0.1
6,638,189 + Pf6N2E2_5820 0.37 +1.6
6,638,190 - Pf6N2E2_5820 0.37 -1.5
6,638,190 - Pf6N2E2_5820 0.37 -0.6
6,638,320 - Pf6N2E2_5820 0.53 +0.6
6,638,516 + Pf6N2E2_5820 0.78 +0.7
6,638,688 + +0.2
6,638,688 + -0.6

Or see this region's nucleotide sequence