Experiment: Nitrate reduction, replicate C 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_5592 and Pf6N2E2_5593 are separated by 55 nucleotides Pf6N2E2_5593 and Pf6N2E2_5594 are separated by 107 nucleotides Pf6N2E2_5594 and Pf6N2E2_5595 overlap by 4 nucleotides 
        Pf6N2E2_5592 - DNA-binding response regulator, LuxR family, at 6,409,737 to 6,410,363 
        _5592 
         
        
        Pf6N2E2_5593 - Leucyl-tRNA synthetase, at 6,410,419 to 6,410,601 
        _5593 
         
        
        Pf6N2E2_5594 - Endonuclease III (EC 4.2.99.18), at 6,410,709 to 6,411,347 
        _5594 
         
        
        Pf6N2E2_5595 - Electron transport complex protein RnfE, at 6,411,344 to 6,411,832 
        _5595 
         Position (kb) 6410 
6411 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 at 6409.491 kb on + strand at 6409.619 kb on + strand at 6409.619 kb on + strand at 6409.626 kb on + strand at 6409.627 kb on - strand at 6409.677 kb on - strand at 6409.691 kb on + strand at 6409.786 kb on + strand at 6409.812 kb on + strand, within Pf6N2E2_5592 at 6409.871 kb on + strand, within Pf6N2E2_5592 at 6410.034 kb on + strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.057 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.069 kb on - strand, within Pf6N2E2_5592 at 6410.126 kb on + strand, within Pf6N2E2_5592 at 6410.276 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.281 kb on - strand, within Pf6N2E2_5592 at 6410.380 kb on + strand at 6410.380 kb on + strand at 6410.387 kb on + strand at 6410.413 kb on + strand at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.618 kb on + strand at 6410.619 kb on - strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.735 kb on - strand at 6410.742 kb on + strand at 6410.743 kb on - strand at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.155 kb on - strand, within Pf6N2E2_5594 at 6411.155 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.272 kb on - strand, within Pf6N2E2_5594 at 6411.321 kb on + strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nitrate reduction, replicate C remove 6,409,491 +  +0.6 6,409,619 +  -2.7 6,409,619 +  -0.3 6,409,626 +  +0.6 6,409,627 -  +1.6 6,409,677 -  +0.1 6,409,691 +  -0.4 6,409,786 +  -1.4 6,409,812 +  Pf6N2E2_5592 0.12  -0.8 6,409,871 +  Pf6N2E2_5592 0.21  -0.3 6,410,034 +  Pf6N2E2_5592 0.47  +1.8 6,410,035 -  Pf6N2E2_5592 0.48  -0.7 6,410,035 -  Pf6N2E2_5592 0.48  -1.5 6,410,035 -  Pf6N2E2_5592 0.48  -0.6 6,410,038 +  Pf6N2E2_5592 0.48  -0.3 6,410,038 +  Pf6N2E2_5592 0.48  -0.7 6,410,038 +  Pf6N2E2_5592 0.48  +0.1 6,410,038 +  Pf6N2E2_5592 0.48  -0.3 6,410,038 +  Pf6N2E2_5592 0.48  -0.7 6,410,038 +  Pf6N2E2_5592 0.48  -0.4 6,410,038 +  Pf6N2E2_5592 0.48  -0.6 6,410,039 -  Pf6N2E2_5592 0.48  -0.3 6,410,039 -  Pf6N2E2_5592 0.48  +0.3 6,410,039 -  Pf6N2E2_5592 0.48  -0.0 6,410,039 -  Pf6N2E2_5592 0.48  -0.3 6,410,039 -  Pf6N2E2_5592 0.48  -0.1 6,410,057 +  Pf6N2E2_5592 0.51  -0.8 6,410,068 +  Pf6N2E2_5592 0.53  -0.2 6,410,068 +  Pf6N2E2_5592 0.53  -0.1 6,410,068 +  Pf6N2E2_5592 0.53  +0.4 6,410,069 -  Pf6N2E2_5592 0.53  +1.6 6,410,126 +  Pf6N2E2_5592 0.62  -0.3 6,410,276 +  Pf6N2E2_5592 0.86  +0.6 6,410,280 +  Pf6N2E2_5592 0.87  +0.6 6,410,280 +  Pf6N2E2_5592 0.87  -0.8 6,410,280 +  Pf6N2E2_5592 0.87  -0.1 6,410,281 -  Pf6N2E2_5592 0.87  +1.6 6,410,380 +  -0.3 6,410,380 +  -0.9 6,410,387 +  -0.9 6,410,413 +  -2.5 6,410,572 +  Pf6N2E2_5593 0.84  +1.6 6,410,572 +  Pf6N2E2_5593 0.84  -1.0 6,410,572 +  Pf6N2E2_5593 0.84  -0.5 6,410,573 -  Pf6N2E2_5593 0.84  -0.6 6,410,573 -  Pf6N2E2_5593 0.84  -1.7 6,410,573 -  Pf6N2E2_5593 0.84  +1.6 6,410,573 -  Pf6N2E2_5593 0.84  -1.9 6,410,573 -  Pf6N2E2_5593 0.84  -3.2 6,410,618 +  -2.5 6,410,619 -  +0.6 6,410,640 +  -1.6 6,410,640 +  +0.3 6,410,640 +  -1.9 6,410,640 +  -0.4 6,410,641 -  -0.6 6,410,641 -  -0.9 6,410,641 -  +0.2 6,410,641 -  +0.8 6,410,641 -  +1.6 6,410,641 -  +0.4 6,410,641 -  -1.2 6,410,641 -  -0.2 6,410,641 -  -2.3 6,410,735 -  +1.9 6,410,742 +  -0.2 6,410,743 -  -0.3 6,411,124 +  Pf6N2E2_5594 0.65  +0.6 6,411,124 +  Pf6N2E2_5594 0.65  +1.3 6,411,124 +  Pf6N2E2_5594 0.65  -0.6 6,411,154 +  Pf6N2E2_5594 0.70  +0.7 6,411,154 +  Pf6N2E2_5594 0.70  +1.0 6,411,154 +  Pf6N2E2_5594 0.70  -0.2 6,411,154 +  Pf6N2E2_5594 0.70  -0.5 6,411,155 -  Pf6N2E2_5594 0.70  -1.9 6,411,155 -  Pf6N2E2_5594 0.70  -0.9 6,411,171 -  Pf6N2E2_5594 0.72  -0.7 6,411,171 -  Pf6N2E2_5594 0.72  -0.7 6,411,171 -  Pf6N2E2_5594 0.72  -0.2 6,411,272 -  Pf6N2E2_5594 0.88  -0.8 6,411,321 +  +0.8 
Or see this region's nucleotide sequence