Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5592 and Pf6N2E2_5593 are separated by 55 nucleotides Pf6N2E2_5593 and Pf6N2E2_5594 are separated by 107 nucleotides Pf6N2E2_5594 and Pf6N2E2_5595 overlap by 4 nucleotides
Pf6N2E2_5592 - DNA-binding response regulator, LuxR family, at 6,409,737 to 6,410,363
_5592
Pf6N2E2_5593 - Leucyl-tRNA synthetase, at 6,410,419 to 6,410,601
_5593
Pf6N2E2_5594 - Endonuclease III (EC 4.2.99.18), at 6,410,709 to 6,411,347
_5594
Pf6N2E2_5595 - Electron transport complex protein RnfE, at 6,411,344 to 6,411,832
_5595
Position (kb)
6410
6411 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6409.491 kb on + strand at 6409.619 kb on + strand at 6409.619 kb on + strand at 6409.626 kb on + strand at 6409.627 kb on - strand at 6409.677 kb on - strand at 6409.691 kb on + strand at 6409.786 kb on + strand at 6409.812 kb on + strand, within Pf6N2E2_5592 at 6409.871 kb on + strand, within Pf6N2E2_5592 at 6410.034 kb on + strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.057 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.069 kb on - strand, within Pf6N2E2_5592 at 6410.126 kb on + strand, within Pf6N2E2_5592 at 6410.276 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.281 kb on - strand, within Pf6N2E2_5592 at 6410.380 kb on + strand at 6410.380 kb on + strand at 6410.387 kb on + strand at 6410.413 kb on + strand at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.618 kb on + strand at 6410.619 kb on - strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.735 kb on - strand at 6410.742 kb on + strand at 6410.743 kb on - strand at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.155 kb on - strand, within Pf6N2E2_5594 at 6411.155 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.272 kb on - strand, within Pf6N2E2_5594 at 6411.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 6,409,491 + +0.6 6,409,619 + -2.7 6,409,619 + -0.3 6,409,626 + +0.6 6,409,627 - +1.6 6,409,677 - +0.1 6,409,691 + -0.4 6,409,786 + -1.4 6,409,812 + Pf6N2E2_5592 0.12 -0.8 6,409,871 + Pf6N2E2_5592 0.21 -0.3 6,410,034 + Pf6N2E2_5592 0.47 +1.8 6,410,035 - Pf6N2E2_5592 0.48 -0.7 6,410,035 - Pf6N2E2_5592 0.48 -1.5 6,410,035 - Pf6N2E2_5592 0.48 -0.6 6,410,038 + Pf6N2E2_5592 0.48 -0.3 6,410,038 + Pf6N2E2_5592 0.48 -0.7 6,410,038 + Pf6N2E2_5592 0.48 +0.1 6,410,038 + Pf6N2E2_5592 0.48 -0.3 6,410,038 + Pf6N2E2_5592 0.48 -0.7 6,410,038 + Pf6N2E2_5592 0.48 -0.4 6,410,038 + Pf6N2E2_5592 0.48 -0.6 6,410,039 - Pf6N2E2_5592 0.48 -0.3 6,410,039 - Pf6N2E2_5592 0.48 +0.3 6,410,039 - Pf6N2E2_5592 0.48 -0.0 6,410,039 - Pf6N2E2_5592 0.48 -0.3 6,410,039 - Pf6N2E2_5592 0.48 -0.1 6,410,057 + Pf6N2E2_5592 0.51 -0.8 6,410,068 + Pf6N2E2_5592 0.53 -0.2 6,410,068 + Pf6N2E2_5592 0.53 -0.1 6,410,068 + Pf6N2E2_5592 0.53 +0.4 6,410,069 - Pf6N2E2_5592 0.53 +1.6 6,410,126 + Pf6N2E2_5592 0.62 -0.3 6,410,276 + Pf6N2E2_5592 0.86 +0.6 6,410,280 + Pf6N2E2_5592 0.87 +0.6 6,410,280 + Pf6N2E2_5592 0.87 -0.8 6,410,280 + Pf6N2E2_5592 0.87 -0.1 6,410,281 - Pf6N2E2_5592 0.87 +1.6 6,410,380 + -0.3 6,410,380 + -0.9 6,410,387 + -0.9 6,410,413 + -2.5 6,410,572 + Pf6N2E2_5593 0.84 +1.6 6,410,572 + Pf6N2E2_5593 0.84 -1.0 6,410,572 + Pf6N2E2_5593 0.84 -0.5 6,410,573 - Pf6N2E2_5593 0.84 -0.6 6,410,573 - Pf6N2E2_5593 0.84 -1.7 6,410,573 - Pf6N2E2_5593 0.84 +1.6 6,410,573 - Pf6N2E2_5593 0.84 -1.9 6,410,573 - Pf6N2E2_5593 0.84 -3.2 6,410,618 + -2.5 6,410,619 - +0.6 6,410,640 + -1.6 6,410,640 + +0.3 6,410,640 + -1.9 6,410,640 + -0.4 6,410,641 - -0.6 6,410,641 - -0.9 6,410,641 - +0.2 6,410,641 - +0.8 6,410,641 - +1.6 6,410,641 - +0.4 6,410,641 - -1.2 6,410,641 - -0.2 6,410,641 - -2.3 6,410,735 - +1.9 6,410,742 + -0.2 6,410,743 - -0.3 6,411,124 + Pf6N2E2_5594 0.65 +0.6 6,411,124 + Pf6N2E2_5594 0.65 +1.3 6,411,124 + Pf6N2E2_5594 0.65 -0.6 6,411,154 + Pf6N2E2_5594 0.70 +0.7 6,411,154 + Pf6N2E2_5594 0.70 +1.0 6,411,154 + Pf6N2E2_5594 0.70 -0.2 6,411,154 + Pf6N2E2_5594 0.70 -0.5 6,411,155 - Pf6N2E2_5594 0.70 -1.9 6,411,155 - Pf6N2E2_5594 0.70 -0.9 6,411,171 - Pf6N2E2_5594 0.72 -0.7 6,411,171 - Pf6N2E2_5594 0.72 -0.7 6,411,171 - Pf6N2E2_5594 0.72 -0.2 6,411,272 - Pf6N2E2_5594 0.88 -0.8 6,411,321 + +0.8
Or see this region's nucleotide sequence