Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4656 and Pf6N2E2_4657 are separated by 24 nucleotides Pf6N2E2_4657 and Pf6N2E2_4658 are separated by 31 nucleotides Pf6N2E2_4658 and Pf6N2E2_4659 are separated by 56 nucleotides Pf6N2E2_4659 and Pf6N2E2_4660 are separated by 162 nucleotides
Pf6N2E2_4656 - D-erythrose-4-phosphate dehydrogenase, at 5,386,570 to 5,387,625
_4656
Pf6N2E2_4657 - Phosphoglycerate kinase (EC 2.7.2.3), at 5,387,650 to 5,388,813
_4657
Pf6N2E2_4658 - FIG00955006: hypothetical protein, at 5,388,845 to 5,389,069
_4658
Pf6N2E2_4659 - Lipoprotein, putative, at 5,389,126 to 5,389,458
_4659
Pf6N2E2_4660 - Fructose-bisphosphate aldolase class II (EC 4.1.2.13), at 5,389,621 to 5,390,685
_4660
Position (kb)
5387
5388
5389 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5386.789 kb on - strand, within Pf6N2E2_4656 at 5387.623 kb on + strand at 5388.957 kb on - strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.028 kb on + strand, within Pf6N2E2_4658 at 5389.029 kb on - strand, within Pf6N2E2_4658 at 5389.029 kb on - strand, within Pf6N2E2_4658 at 5389.083 kb on + strand at 5389.083 kb on + strand at 5389.084 kb on - strand at 5389.084 kb on - strand at 5389.084 kb on - strand at 5389.084 kb on - strand at 5389.157 kb on + strand at 5389.157 kb on + strand at 5389.157 kb on + strand at 5389.190 kb on + strand, within Pf6N2E2_4659 at 5389.191 kb on - strand, within Pf6N2E2_4659 at 5389.357 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.408 kb on + strand, within Pf6N2E2_4659 at 5389.490 kb on + strand at 5389.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 5,386,789 - Pf6N2E2_4656 0.21 -0.1 5,387,623 + +0.6 5,388,957 - Pf6N2E2_4658 0.50 -0.2 5,389,028 + Pf6N2E2_4658 0.81 +0.7 5,389,028 + Pf6N2E2_4658 0.81 -0.9 5,389,028 + Pf6N2E2_4658 0.81 -0.1 5,389,028 + Pf6N2E2_4658 0.81 -1.0 5,389,028 + Pf6N2E2_4658 0.81 -0.8 5,389,028 + Pf6N2E2_4658 0.81 -0.5 5,389,028 + Pf6N2E2_4658 0.81 -0.1 5,389,029 - Pf6N2E2_4658 0.82 -1.2 5,389,029 - Pf6N2E2_4658 0.82 +0.7 5,389,083 + +1.2 5,389,083 + +0.2 5,389,084 - -1.5 5,389,084 - -0.2 5,389,084 - +0.5 5,389,084 - -1.0 5,389,157 + +2.8 5,389,157 + -0.1 5,389,157 + -0.0 5,389,190 + Pf6N2E2_4659 0.19 -0.5 5,389,191 - Pf6N2E2_4659 0.20 +0.8 5,389,357 + Pf6N2E2_4659 0.69 +0.5 5,389,408 + Pf6N2E2_4659 0.85 -0.2 5,389,408 + Pf6N2E2_4659 0.85 -1.2 5,389,408 + Pf6N2E2_4659 0.85 +1.2 5,389,408 + Pf6N2E2_4659 0.85 -0.9 5,389,408 + Pf6N2E2_4659 0.85 +0.2 5,389,408 + Pf6N2E2_4659 0.85 +0.3 5,389,408 + Pf6N2E2_4659 0.85 +0.2 5,389,490 + +0.7 5,389,492 + +3.0
Or see this region's nucleotide sequence