Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3936 and Pf6N2E2_3937 are separated by 165 nucleotides Pf6N2E2_3937 and Pf6N2E2_3938 are separated by 137 nucleotides Pf6N2E2_3938 and Pf6N2E2_3939 are separated by 144 nucleotides Pf6N2E2_3939 and Pf6N2E2_3940 are separated by 37 nucleotides
Pf6N2E2_3936 - Outer membrane protein assembly factor YaeT precursor, at 4,573,059 to 4,573,976
_3936
Pf6N2E2_3937 - Ribose 5-phosphate isomerase A (EC 5.3.1.6), at 4,574,142 to 4,574,813
_3937
Pf6N2E2_3938 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 4,574,951 to 4,576,465
_3938
Pf6N2E2_3939 - Predicted integral membrane protein, at 4,576,610 to 4,577,035
_3939
Pf6N2E2_3940 - Phosphoserine phosphatase (EC 3.1.3.3), at 4,577,073 to 4,577,729
_3940
Position (kb)
4574
4575
4576
4577 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4573.951 kb on + strand at 4573.951 kb on + strand at 4573.952 kb on - strand at 4573.952 kb on - strand at 4573.952 kb on - strand at 4573.952 kb on - strand at 4573.952 kb on - strand at 4573.952 kb on - strand at 4573.974 kb on + strand at 4573.974 kb on + strand at 4573.974 kb on + strand at 4573.974 kb on + strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.975 kb on - strand at 4573.978 kb on + strand at 4573.978 kb on + strand at 4573.979 kb on - strand at 4573.979 kb on - strand at 4573.979 kb on - strand at 4573.979 kb on - strand at 4574.840 kb on - strand at 4574.856 kb on - strand at 4574.856 kb on - strand at 4574.856 kb on - strand at 4574.856 kb on - strand at 4574.856 kb on - strand at 4574.858 kb on - strand at 4574.858 kb on - strand at 4574.897 kb on - strand at 4574.897 kb on - strand at 4574.963 kb on + strand at 4574.963 kb on + strand at 4574.963 kb on + strand at 4574.964 kb on - strand at 4574.964 kb on - strand at 4574.964 kb on - strand at 4574.993 kb on + strand at 4575.116 kb on + strand, within Pf6N2E2_3938 at 4575.116 kb on + strand, within Pf6N2E2_3938 at 4575.116 kb on + strand, within Pf6N2E2_3938 at 4575.116 kb on + strand, within Pf6N2E2_3938 at 4575.116 kb on + strand, within Pf6N2E2_3938 at 4575.117 kb on - strand, within Pf6N2E2_3938 at 4575.117 kb on - strand, within Pf6N2E2_3938 at 4575.117 kb on - strand, within Pf6N2E2_3938 at 4575.117 kb on - strand, within Pf6N2E2_3938 at 4575.216 kb on + strand, within Pf6N2E2_3938 at 4575.217 kb on - strand, within Pf6N2E2_3938 at 4575.217 kb on - strand, within Pf6N2E2_3938 at 4575.217 kb on - strand, within Pf6N2E2_3938 at 4575.338 kb on + strand, within Pf6N2E2_3938 at 4575.339 kb on - strand, within Pf6N2E2_3938 at 4575.368 kb on + strand, within Pf6N2E2_3938 at 4575.368 kb on + strand, within Pf6N2E2_3938 at 4575.369 kb on - strand, within Pf6N2E2_3938 at 4575.369 kb on - strand, within Pf6N2E2_3938 at 4575.374 kb on + strand, within Pf6N2E2_3938 at 4575.374 kb on + strand, within Pf6N2E2_3938 at 4575.374 kb on + strand, within Pf6N2E2_3938 at 4575.374 kb on + strand, within Pf6N2E2_3938 at 4575.374 kb on + strand, within Pf6N2E2_3938 at 4575.374 kb on + strand, within Pf6N2E2_3938 at 4575.374 kb on + strand, within Pf6N2E2_3938 at 4575.375 kb on - strand, within Pf6N2E2_3938 at 4575.375 kb on - strand, within Pf6N2E2_3938 at 4575.375 kb on - strand, within Pf6N2E2_3938 at 4575.375 kb on - strand, within Pf6N2E2_3938 at 4575.375 kb on - strand, within Pf6N2E2_3938 at 4575.386 kb on + strand, within Pf6N2E2_3938 at 4575.516 kb on - strand, within Pf6N2E2_3938 at 4575.524 kb on + strand, within Pf6N2E2_3938 at 4575.525 kb on - strand, within Pf6N2E2_3938 at 4575.609 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.749 kb on - strand, within Pf6N2E2_3938 at 4575.749 kb on - strand, within Pf6N2E2_3938 at 4575.815 kb on + strand, within Pf6N2E2_3938 at 4576.025 kb on + strand, within Pf6N2E2_3938 at 4576.026 kb on - strand, within Pf6N2E2_3938 at 4576.032 kb on - strand, within Pf6N2E2_3938 at 4576.032 kb on - strand, within Pf6N2E2_3938 at 4576.032 kb on - strand, within Pf6N2E2_3938 at 4576.435 kb on + strand at 4576.445 kb on + strand at 4576.445 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.596 kb on - strand at 4576.877 kb on + strand, within Pf6N2E2_3939 at 4576.877 kb on + strand, within Pf6N2E2_3939 at 4576.877 kb on + strand, within Pf6N2E2_3939 at 4576.877 kb on + strand, within Pf6N2E2_3939 at 4576.878 kb on - strand, within Pf6N2E2_3939 at 4576.878 kb on - strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.961 kb on - strand, within Pf6N2E2_3939 at 4576.961 kb on - strand, within Pf6N2E2_3939 at 4577.065 kb on + strand at 4577.201 kb on - strand, within Pf6N2E2_3940 at 4577.201 kb on - strand, within Pf6N2E2_3940 at 4577.206 kb on + strand, within Pf6N2E2_3940 at 4577.206 kb on + strand, within Pf6N2E2_3940 at 4577.206 kb on + strand, within Pf6N2E2_3940 at 4577.207 kb on - strand, within Pf6N2E2_3940 at 4577.207 kb on - strand, within Pf6N2E2_3940 at 4577.207 kb on - strand, within Pf6N2E2_3940 at 4577.207 kb on - strand, within Pf6N2E2_3940 at 4577.306 kb on - strand, within Pf6N2E2_3940
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,573,951 + -0.1 4,573,951 + +0.0 4,573,952 - +0.7 4,573,952 - -0.7 4,573,952 - +0.4 4,573,952 - -0.3 4,573,952 - +0.6 4,573,952 - +0.8 4,573,974 + -0.6 4,573,974 + -1.1 4,573,974 + -0.5 4,573,974 + +0.5 4,573,975 - +1.5 4,573,975 - +0.1 4,573,975 - -0.7 4,573,975 - -1.3 4,573,975 - -1.1 4,573,975 - +1.5 4,573,975 - -1.8 4,573,975 - -0.4 4,573,975 - +0.5 4,573,975 - -0.8 4,573,975 - +0.9 4,573,975 - -1.5 4,573,978 + -0.4 4,573,978 + -1.2 4,573,979 - +1.5 4,573,979 - -0.1 4,573,979 - -0.6 4,573,979 - -0.1 4,574,840 - +0.3 4,574,856 - -0.7 4,574,856 - -0.7 4,574,856 - +0.2 4,574,856 - -0.2 4,574,856 - -0.8 4,574,858 - +0.4 4,574,858 - +0.5 4,574,897 - +0.1 4,574,897 - -0.1 4,574,963 + -0.3 4,574,963 + -2.6 4,574,963 + -1.3 4,574,964 - -1.9 4,574,964 - -1.4 4,574,964 - -1.1 4,574,993 + +1.5 4,575,116 + Pf6N2E2_3938 0.11 -0.7 4,575,116 + Pf6N2E2_3938 0.11 -2.0 4,575,116 + Pf6N2E2_3938 0.11 +1.5 4,575,116 + Pf6N2E2_3938 0.11 -1.2 4,575,116 + Pf6N2E2_3938 0.11 -0.5 4,575,117 - Pf6N2E2_3938 0.11 -0.7 4,575,117 - Pf6N2E2_3938 0.11 +0.5 4,575,117 - Pf6N2E2_3938 0.11 -1.8 4,575,117 - Pf6N2E2_3938 0.11 -1.4 4,575,216 + Pf6N2E2_3938 0.17 -0.9 4,575,217 - Pf6N2E2_3938 0.18 -1.3 4,575,217 - Pf6N2E2_3938 0.18 -0.9 4,575,217 - Pf6N2E2_3938 0.18 -0.4 4,575,338 + Pf6N2E2_3938 0.26 -0.5 4,575,339 - Pf6N2E2_3938 0.26 -1.6 4,575,368 + Pf6N2E2_3938 0.28 -0.2 4,575,368 + Pf6N2E2_3938 0.28 -2.3 4,575,369 - Pf6N2E2_3938 0.28 -1.8 4,575,369 - Pf6N2E2_3938 0.28 -0.9 4,575,374 + Pf6N2E2_3938 0.28 -2.3 4,575,374 + Pf6N2E2_3938 0.28 -1.3 4,575,374 + Pf6N2E2_3938 0.28 +0.5 4,575,374 + Pf6N2E2_3938 0.28 +0.5 4,575,374 + Pf6N2E2_3938 0.28 -0.9 4,575,374 + Pf6N2E2_3938 0.28 -1.2 4,575,374 + Pf6N2E2_3938 0.28 -0.9 4,575,375 - Pf6N2E2_3938 0.28 -0.5 4,575,375 - Pf6N2E2_3938 0.28 -0.9 4,575,375 - Pf6N2E2_3938 0.28 -1.7 4,575,375 - Pf6N2E2_3938 0.28 -0.1 4,575,375 - Pf6N2E2_3938 0.28 -1.0 4,575,386 + Pf6N2E2_3938 0.29 -1.7 4,575,516 - Pf6N2E2_3938 0.37 -0.9 4,575,524 + Pf6N2E2_3938 0.38 -0.6 4,575,525 - Pf6N2E2_3938 0.38 -1.7 4,575,609 + Pf6N2E2_3938 0.43 -1.2 4,575,692 + Pf6N2E2_3938 0.49 -2.0 4,575,692 + Pf6N2E2_3938 0.49 -0.9 4,575,692 + Pf6N2E2_3938 0.49 -1.5 4,575,692 + Pf6N2E2_3938 0.49 -1.2 4,575,692 + Pf6N2E2_3938 0.49 +1.9 4,575,692 + Pf6N2E2_3938 0.49 +1.5 4,575,692 + Pf6N2E2_3938 0.49 -0.9 4,575,692 + Pf6N2E2_3938 0.49 +0.3 4,575,692 + Pf6N2E2_3938 0.49 -0.3 4,575,692 + Pf6N2E2_3938 0.49 -1.4 4,575,693 - Pf6N2E2_3938 0.49 -0.9 4,575,693 - Pf6N2E2_3938 0.49 -1.1 4,575,693 - Pf6N2E2_3938 0.49 -2.2 4,575,693 - Pf6N2E2_3938 0.49 -2.0 4,575,693 - Pf6N2E2_3938 0.49 -0.5 4,575,749 - Pf6N2E2_3938 0.53 -1.3 4,575,749 - Pf6N2E2_3938 0.53 -1.3 4,575,815 + Pf6N2E2_3938 0.57 +0.1 4,576,025 + Pf6N2E2_3938 0.71 -1.6 4,576,026 - Pf6N2E2_3938 0.71 -2.6 4,576,032 - Pf6N2E2_3938 0.71 -2.3 4,576,032 - Pf6N2E2_3938 0.71 -1.1 4,576,032 - Pf6N2E2_3938 0.71 -0.3 4,576,435 + +0.2 4,576,445 + -0.4 4,576,445 + -0.7 4,576,574 + -0.4 4,576,574 + -0.2 4,576,574 + +1.5 4,576,574 + -0.9 4,576,574 + -1.0 4,576,574 + +0.2 4,576,575 - -0.9 4,576,575 - -0.6 4,576,575 - +0.4 4,576,575 - +0.3 4,576,575 - +0.9 4,576,575 - +0.1 4,576,575 - -0.1 4,576,596 - -0.8 4,576,877 + Pf6N2E2_3939 0.63 +0.6 4,576,877 + Pf6N2E2_3939 0.63 +0.4 4,576,877 + Pf6N2E2_3939 0.63 -0.1 4,576,877 + Pf6N2E2_3939 0.63 -1.0 4,576,878 - Pf6N2E2_3939 0.63 +0.1 4,576,878 - Pf6N2E2_3939 0.63 -0.5 4,576,879 + Pf6N2E2_3939 0.63 -2.6 4,576,879 + Pf6N2E2_3939 0.63 +0.4 4,576,879 + Pf6N2E2_3939 0.63 +0.6 4,576,879 + Pf6N2E2_3939 0.63 -0.5 4,576,879 + Pf6N2E2_3939 0.63 -0.1 4,576,880 - Pf6N2E2_3939 0.63 -0.5 4,576,880 - Pf6N2E2_3939 0.63 +0.1 4,576,880 - Pf6N2E2_3939 0.63 +1.1 4,576,880 - Pf6N2E2_3939 0.63 +1.1 4,576,880 - Pf6N2E2_3939 0.63 -0.8 4,576,961 - Pf6N2E2_3939 0.82 -0.9 4,576,961 - Pf6N2E2_3939 0.82 +1.1 4,577,065 + -0.6 4,577,201 - Pf6N2E2_3940 0.19 -3.5 4,577,201 - Pf6N2E2_3940 0.19 -2.8 4,577,206 + Pf6N2E2_3940 0.20 -2.9 4,577,206 + Pf6N2E2_3940 0.20 -3.6 4,577,206 + Pf6N2E2_3940 0.20 -2.8 4,577,207 - Pf6N2E2_3940 0.20 +0.5 4,577,207 - Pf6N2E2_3940 0.20 -2.2 4,577,207 - Pf6N2E2_3940 0.20 +0.1 4,577,207 - Pf6N2E2_3940 0.20 +0.5 4,577,306 - Pf6N2E2_3940 0.35 -1.5
Or see this region's nucleotide sequence