Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3531 and Pf6N2E2_3532 overlap by 1 nucleotides Pf6N2E2_3532 and Pf6N2E2_3533 overlap by 4 nucleotides Pf6N2E2_3533 and Pf6N2E2_3534 overlap by 8 nucleotides
Pf6N2E2_3531 - Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase, at 4,121,025 to 4,122,713
_3531
Pf6N2E2_3532 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 4,122,713 to 4,123,447
_3532
Pf6N2E2_3533 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 4,123,444 to 4,124,070
_3533
Pf6N2E2_3534 - Cobalamin biosynthesis protein CobG, at 4,124,063 to 4,125,331
_3534
Position (kb)
4123
4124
4125 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4122.551 kb on - strand at 4122.583 kb on + strand at 4122.583 kb on + strand at 4122.583 kb on + strand at 4122.584 kb on - strand at 4122.757 kb on - strand at 4122.828 kb on + strand, within Pf6N2E2_3532 at 4123.063 kb on - strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.095 kb on + strand, within Pf6N2E2_3532 at 4123.095 kb on + strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.212 kb on + strand, within Pf6N2E2_3532 at 4123.213 kb on - strand, within Pf6N2E2_3532 at 4123.252 kb on - strand, within Pf6N2E2_3532 at 4123.339 kb on - strand, within Pf6N2E2_3532 at 4123.339 kb on - strand, within Pf6N2E2_3532 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.119 kb on + strand at 4124.119 kb on + strand at 4124.119 kb on + strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.123 kb on + strand at 4124.123 kb on + strand at 4124.124 kb on - strand at 4124.124 kb on - strand at 4124.124 kb on - strand at 4124.175 kb on + strand at 4124.175 kb on + strand at 4124.494 kb on + strand, within Pf6N2E2_3534 at 4124.494 kb on + strand, within Pf6N2E2_3534 at 4124.495 kb on - strand, within Pf6N2E2_3534 at 4124.495 kb on - strand, within Pf6N2E2_3534 at 4125.035 kb on - strand, within Pf6N2E2_3534
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 4,122,551 - -2.6 4,122,583 + -0.1 4,122,583 + +0.3 4,122,583 + +1.5 4,122,584 - +1.5 4,122,757 - -0.7 4,122,828 + Pf6N2E2_3532 0.16 -0.1 4,123,063 - Pf6N2E2_3532 0.48 +0.3 4,123,089 + Pf6N2E2_3532 0.51 +0.5 4,123,089 + Pf6N2E2_3532 0.51 +0.8 4,123,089 + Pf6N2E2_3532 0.51 +1.3 4,123,089 + Pf6N2E2_3532 0.51 -0.1 4,123,089 + Pf6N2E2_3532 0.51 +2.5 4,123,089 + Pf6N2E2_3532 0.51 -0.3 4,123,089 + Pf6N2E2_3532 0.51 +0.5 4,123,090 - Pf6N2E2_3532 0.51 +0.2 4,123,090 - Pf6N2E2_3532 0.51 +1.5 4,123,090 - Pf6N2E2_3532 0.51 +1.5 4,123,095 + Pf6N2E2_3532 0.52 +0.5 4,123,095 + Pf6N2E2_3532 0.52 -0.2 4,123,096 - Pf6N2E2_3532 0.52 +1.5 4,123,096 - Pf6N2E2_3532 0.52 +1.5 4,123,096 - Pf6N2E2_3532 0.52 -1.3 4,123,096 - Pf6N2E2_3532 0.52 +2.5 4,123,096 - Pf6N2E2_3532 0.52 +1.5 4,123,096 - Pf6N2E2_3532 0.52 +0.3 4,123,096 - Pf6N2E2_3532 0.52 +1.5 4,123,212 + Pf6N2E2_3532 0.68 -1.7 4,123,213 - Pf6N2E2_3532 0.68 +1.0 4,123,252 - Pf6N2E2_3532 0.73 +0.5 4,123,339 - Pf6N2E2_3532 0.85 -1.3 4,123,339 - Pf6N2E2_3532 0.85 -0.3 4,123,646 + Pf6N2E2_3533 0.32 -1.9 4,123,646 + Pf6N2E2_3533 0.32 +2.5 4,123,646 + Pf6N2E2_3533 0.32 -1.1 4,123,647 - Pf6N2E2_3533 0.32 -0.2 4,123,647 - Pf6N2E2_3533 0.32 +1.5 4,123,647 - Pf6N2E2_3533 0.32 +1.5 4,123,647 - Pf6N2E2_3533 0.32 -0.5 4,123,647 - Pf6N2E2_3533 0.32 +0.5 4,124,060 + -1.3 4,124,060 + -0.1 4,124,060 + +0.6 4,124,060 + +0.5 4,124,060 + +0.0 4,124,060 + -0.1 4,124,060 + +2.5 4,124,061 - +0.5 4,124,061 - -0.2 4,124,061 - +1.5 4,124,061 - -1.5 4,124,119 + -0.1 4,124,119 + +1.5 4,124,119 + -0.5 4,124,120 - -0.4 4,124,120 - -0.8 4,124,120 - -2.5 4,124,120 - -2.1 4,124,120 - -2.7 4,124,123 + +0.1 4,124,123 + +1.5 4,124,124 - -0.6 4,124,124 - -1.3 4,124,124 - -0.1 4,124,175 + +1.5 4,124,175 + +1.5 4,124,494 + Pf6N2E2_3534 0.34 +1.5 4,124,494 + Pf6N2E2_3534 0.34 -0.2 4,124,495 - Pf6N2E2_3534 0.34 +0.5 4,124,495 - Pf6N2E2_3534 0.34 -1.5 4,125,035 - Pf6N2E2_3534 0.77 -2.3
Or see this region's nucleotide sequence